Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy
<i>Haemophilus influenzae</i> invasive disease is a severe infection that needs rapid antibiotic therapy. The aim of the study was to perform and evaluate the serotype distribution, antibiotic susceptibility and molecular characteristics of 392 <i>H. influenzae</i> invasive i...
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MDPI AG
2023-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/315 |
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| author | Maria Giufrè Rita Cardines Manuela Marra Maria Carollo Marina Cerquetti Paola Stefanelli |
| author_facet | Maria Giufrè Rita Cardines Manuela Marra Maria Carollo Marina Cerquetti Paola Stefanelli |
| author_sort | Maria Giufrè |
| collection | DOAJ |
| description | <i>Haemophilus influenzae</i> invasive disease is a severe infection that needs rapid antibiotic therapy. The aim of the study was to perform and evaluate the serotype distribution, antibiotic susceptibility and molecular characteristics of 392 <i>H. influenzae</i> invasive isolates collected during 2017–2021 in Italy. The majority of isolates were NTHi (305/392, 77.8%), followed by Hib (49/392, 12.5%). Ampicillin resistance was frequently detected (85/392, 21.7%): 12.2% were β-lactamase producers (all <i>bla</i><sub>TEM</sub> except one <i>bla</i><sub>ROB</sub>), 9.4% were β-lactamase-negative ampicillin-resistant (BLNAR), with mutations in the <i>fts</i>I gene. Six isolates were resistant to ciprofloxacin, with substitutions in GyrA and ParC. An MLST analysis revealed the occurrence of international resistant clones, such as ST103 and ST14, highlighting the importance of molecular surveillance. |
| first_indexed | 2024-03-11T08:24:41Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T08:24:41Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-dd754c9c736d4792b1b44c7dcfa4521d2023-11-16T22:14:12ZengMDPI AGMicroorganisms2076-26072023-01-0111231510.3390/microorganisms11020315Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, ItalyMaria Giufrè0Rita Cardines1Manuela Marra2Maria Carollo3Marina Cerquetti4Paola Stefanelli5Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyCore Facilities Technical-Scientific Service (FAST), Istituto Superiore di Sanità, 00161 Rome, ItalyCore Facilities Technical-Scientific Service (FAST), Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy<i>Haemophilus influenzae</i> invasive disease is a severe infection that needs rapid antibiotic therapy. The aim of the study was to perform and evaluate the serotype distribution, antibiotic susceptibility and molecular characteristics of 392 <i>H. influenzae</i> invasive isolates collected during 2017–2021 in Italy. The majority of isolates were NTHi (305/392, 77.8%), followed by Hib (49/392, 12.5%). Ampicillin resistance was frequently detected (85/392, 21.7%): 12.2% were β-lactamase producers (all <i>bla</i><sub>TEM</sub> except one <i>bla</i><sub>ROB</sub>), 9.4% were β-lactamase-negative ampicillin-resistant (BLNAR), with mutations in the <i>fts</i>I gene. Six isolates were resistant to ciprofloxacin, with substitutions in GyrA and ParC. An MLST analysis revealed the occurrence of international resistant clones, such as ST103 and ST14, highlighting the importance of molecular surveillance.https://www.mdpi.com/2076-2607/11/2/315<i>Haemophilus influenzae</i>surveillanceMLSTantibiotic resistancewhole genome sequencingquinolone resistance |
| spellingShingle | Maria Giufrè Rita Cardines Manuela Marra Maria Carollo Marina Cerquetti Paola Stefanelli Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy Microorganisms <i>Haemophilus influenzae</i> surveillance MLST antibiotic resistance whole genome sequencing quinolone resistance |
| title | Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy |
| title_full | Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy |
| title_fullStr | Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy |
| title_full_unstemmed | Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy |
| title_short | Antibiotic Susceptibility and Molecular Typing of Invasive <i>Haemophilus influenzae</i> Isolates, with Emergence of Ciprofloxacin Resistance, 2017–2021, Italy |
| title_sort | antibiotic susceptibility and molecular typing of invasive i haemophilus influenzae i isolates with emergence of ciprofloxacin resistance 2017 2021 italy |
| topic | <i>Haemophilus influenzae</i> surveillance MLST antibiotic resistance whole genome sequencing quinolone resistance |
| url | https://www.mdpi.com/2076-2607/11/2/315 |
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