Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase
The catalytic properties of Seqenzym<sup>®</sup> FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was only slightly inhibited by sucrose abo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/13/5/843 |
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| author | Klaudia Karkeszová Milan Polakovič |
| author_facet | Klaudia Karkeszová Milan Polakovič |
| author_sort | Klaudia Karkeszová |
| collection | DOAJ |
| description | The catalytic properties of Seqenzym<sup>®</sup> FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was only slightly inhibited by sucrose above the concentration of 1.5 M. A rather low level of hydrolytic side activity was observed even at sucrose concentrations as low as 0.25 M. In progress curve experiments, the mass yield of fructooligosaccharides (FOS) reached a maximum value of 57% at this sucrose concentration, although it dropped to about 35% later on. At high initial sucrose concentrations up to 2 M, the FOS yield reached a maximum value of approximately 63% at a sucrose conversion of approximately 90%. Although neither the yield nor the conversion changed much later on, the progress of the reaction was manifested by the gradual depletion of shorter chain FOS, 1-kestose and nystose, and the accumulation of 1-β-fructofuranosyl nystose. At initial sucrose concentrations of 2 M, the degree of polymerization expressed through the number of fructosyl units grew from 2.3 at a conversion degree of 87% to 3.1 at a conversion degree of 94%. Compared to other commercial preparations, Seqenzym<sup>®</sup> FT can better produce FOS with a higher degree of polymerization. |
| first_indexed | 2024-03-11T03:52:11Z |
| format | Article |
| id | doaj.art-dd77d1486403454b8e10cbacde57ec0f |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-11T03:52:11Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Catalysts |
| spelling | doaj.art-dd77d1486403454b8e10cbacde57ec0f2023-11-18T00:50:59ZengMDPI AGCatalysts2073-43442023-05-0113584310.3390/catal13050843Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. FructosyltransferaseKlaudia Karkeszová0Milan Polakovič1Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaDepartment of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, SlovakiaThe catalytic properties of Seqenzym<sup>®</sup> FT, a fungal fructosyltransferase heterologously expressed in yeasts, were investigated at a temperature of 55 °C and pH 5.5. The initial rate measurements showed that the transfructosylation rate was only slightly inhibited by sucrose above the concentration of 1.5 M. A rather low level of hydrolytic side activity was observed even at sucrose concentrations as low as 0.25 M. In progress curve experiments, the mass yield of fructooligosaccharides (FOS) reached a maximum value of 57% at this sucrose concentration, although it dropped to about 35% later on. At high initial sucrose concentrations up to 2 M, the FOS yield reached a maximum value of approximately 63% at a sucrose conversion of approximately 90%. Although neither the yield nor the conversion changed much later on, the progress of the reaction was manifested by the gradual depletion of shorter chain FOS, 1-kestose and nystose, and the accumulation of 1-β-fructofuranosyl nystose. At initial sucrose concentrations of 2 M, the degree of polymerization expressed through the number of fructosyl units grew from 2.3 at a conversion degree of 87% to 3.1 at a conversion degree of 94%. Compared to other commercial preparations, Seqenzym<sup>®</sup> FT can better produce FOS with a higher degree of polymerization.https://www.mdpi.com/2073-4344/13/5/843fructooligosaccharidesfructosyl transferaseheterologous expression<i>Aspergillus</i> sp.degree of polymerization |
| spellingShingle | Klaudia Karkeszová Milan Polakovič Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase Catalysts fructooligosaccharides fructosyl transferase heterologous expression <i>Aspergillus</i> sp. degree of polymerization |
| title | Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase |
| title_full | Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase |
| title_fullStr | Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase |
| title_full_unstemmed | Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase |
| title_short | Production of Fructooligosaccharides Using a Commercial Heterologously Expressed <i>Aspergillus</i> sp. Fructosyltransferase |
| title_sort | production of fructooligosaccharides using a commercial heterologously expressed i aspergillus i sp fructosyltransferase |
| topic | fructooligosaccharides fructosyl transferase heterologous expression <i>Aspergillus</i> sp. degree of polymerization |
| url | https://www.mdpi.com/2073-4344/13/5/843 |
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