Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells.
Peptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applicati...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-03-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3062552?pdf=render |
| _version_ | 1818963168080166912 |
|---|---|
| author | Benjamin Macadangdang Ning Zhang Paul E Lund Andrew H Marple Mitsunori Okabe Michael M Gottesman Daniel H Appella Chava Kimchi-Sarfaty |
| author_facet | Benjamin Macadangdang Ning Zhang Paul E Lund Andrew H Marple Mitsunori Okabe Michael M Gottesman Daniel H Appella Chava Kimchi-Sarfaty |
| author_sort | Benjamin Macadangdang |
| collection | DOAJ |
| description | Peptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applications. Here, we present a novel method for the in vitro delivery of antigene PNA to cells. By using a nucleocapsid protein derived from Simian virus 40, we have been able to package PNA into pseudovirions, facilitating the delivery of the packaged PNA into cells. We demonstrate that this system can be used effectively to suppress gene expression associated with multidrug resistance in cancer cells, as shown by RT-PCR, flow cytometry, Western blotting, and cell viability under chemotherapy. The combination of PNA with the SV40-based delivery system is a method for suppressing a gene of interest that could be broadly applied to numerous targets. |
| first_indexed | 2024-12-20T12:40:55Z |
| format | Article |
| id | doaj.art-dd7c724cc74243beaf6fc1084a3a3355 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T12:40:55Z |
| publishDate | 2011-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dd7c724cc74243beaf6fc1084a3a33552022-12-21T19:40:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-03-0163e1798110.1371/journal.pone.0017981Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells.Benjamin MacadangdangNing ZhangPaul E LundAndrew H MarpleMitsunori OkabeMichael M GottesmanDaniel H AppellaChava Kimchi-SarfatyPeptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applications. Here, we present a novel method for the in vitro delivery of antigene PNA to cells. By using a nucleocapsid protein derived from Simian virus 40, we have been able to package PNA into pseudovirions, facilitating the delivery of the packaged PNA into cells. We demonstrate that this system can be used effectively to suppress gene expression associated with multidrug resistance in cancer cells, as shown by RT-PCR, flow cytometry, Western blotting, and cell viability under chemotherapy. The combination of PNA with the SV40-based delivery system is a method for suppressing a gene of interest that could be broadly applied to numerous targets.http://europepmc.org/articles/PMC3062552?pdf=render |
| spellingShingle | Benjamin Macadangdang Ning Zhang Paul E Lund Andrew H Marple Mitsunori Okabe Michael M Gottesman Daniel H Appella Chava Kimchi-Sarfaty Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells. PLoS ONE |
| title | Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells. |
| title_full | Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells. |
| title_fullStr | Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells. |
| title_full_unstemmed | Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells. |
| title_short | Inhibition of multidrug resistance by SV40 pseudovirion delivery of an antigene peptide nucleic acid (PNA) in cultured cells. |
| title_sort | inhibition of multidrug resistance by sv40 pseudovirion delivery of an antigene peptide nucleic acid pna in cultured cells |
| url | http://europepmc.org/articles/PMC3062552?pdf=render |
| work_keys_str_mv | AT benjaminmacadangdang inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT ningzhang inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT paulelund inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT andrewhmarple inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT mitsunoriokabe inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT michaelmgottesman inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT danielhappella inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells AT chavakimchisarfaty inhibitionofmultidrugresistancebysv40pseudoviriondeliveryofanantigenepeptidenucleicacidpnainculturedcells |