Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
Abstract Background Control of Mycobacterium tuberculosis (Mtb) infection requires CD4+ T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation o...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Immunology |
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| Online Access: | http://link.springer.com/article/10.1186/s12865-018-0258-8 |
| _version_ | 1818246597399543808 |
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| author | Zhengzhong Xu Aihong Xia Xin Li Zhaocheng Zhu Yechi Shen Shanshan Jin Tian Lan Yuqing Xie Han Wu Chuang Meng Lin Sun Yuelan Yin Xiang Chen Xinan Jiao |
| author_facet | Zhengzhong Xu Aihong Xia Xin Li Zhaocheng Zhu Yechi Shen Shanshan Jin Tian Lan Yuqing Xie Han Wu Chuang Meng Lin Sun Yuelan Yin Xiang Chen Xinan Jiao |
| author_sort | Zhengzhong Xu |
| collection | DOAJ |
| description | Abstract Background Control of Mycobacterium tuberculosis (Mtb) infection requires CD4+ T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-cell immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial immune responses at early infection time points, but the kinetics of Ag presentation by these cells during these events are incompletely understood. Results In the present study, we evaluated in vivo dynamics of early Ag presentation by murine lymph-node (LN) DCs in response to Mycobacterium bovis bacillus Calmette–Guérin (BCG) Ag85A protein. Results showed that the early Ag-presenting activity of murine DCs induced by M. bovis BCG Ag85A protein in vivo was transient, appearing at 4 h and being barely detectable at 72 h. The transcription levels of CIITA, MHC II and the expression of MHC II molecule on the cell surface increased following BCG infection. Moreover, BCG was found to survive within the inguinal LN DC pool, representing a continuing source of mycobacterial Ag85A protein, with which LN DCs formed Ag85A peptide-MHCII complexes in vivo. Conclusions Our results demonstrate that a decrease in Ag85A peptide production as a result of the inhibition of Ag processing to is largely responsible for the short duration of Ag presentation by LN DCs during BCG infection in vivo. |
| first_indexed | 2024-12-12T14:51:20Z |
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| id | doaj.art-dd7d076e5fb645b1be8135ec3ef63628 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-12T14:51:20Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
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| series | BMC Immunology |
| spelling | doaj.art-dd7d076e5fb645b1be8135ec3ef636282022-12-22T00:21:00ZengBMCBMC Immunology1471-21722018-06-011911810.1186/s12865-018-0258-8Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infectionZhengzhong Xu0Aihong Xia1Xin Li2Zhaocheng Zhu3Yechi Shen4Shanshan Jin5Tian Lan6Yuqing Xie7Han Wu8Chuang Meng9Lin Sun10Yuelan Yin11Xiang Chen12Xinan Jiao13Jiangsu Key Laboratory of Zoonosis, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, MOA of China, Yangzhou UniversityAbstract Background Control of Mycobacterium tuberculosis (Mtb) infection requires CD4+ T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-cell immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial immune responses at early infection time points, but the kinetics of Ag presentation by these cells during these events are incompletely understood. Results In the present study, we evaluated in vivo dynamics of early Ag presentation by murine lymph-node (LN) DCs in response to Mycobacterium bovis bacillus Calmette–Guérin (BCG) Ag85A protein. Results showed that the early Ag-presenting activity of murine DCs induced by M. bovis BCG Ag85A protein in vivo was transient, appearing at 4 h and being barely detectable at 72 h. The transcription levels of CIITA, MHC II and the expression of MHC II molecule on the cell surface increased following BCG infection. Moreover, BCG was found to survive within the inguinal LN DC pool, representing a continuing source of mycobacterial Ag85A protein, with which LN DCs formed Ag85A peptide-MHCII complexes in vivo. Conclusions Our results demonstrate that a decrease in Ag85A peptide production as a result of the inhibition of Ag processing to is largely responsible for the short duration of Ag presentation by LN DCs during BCG infection in vivo.http://link.springer.com/article/10.1186/s12865-018-0258-8Ag-presenting activityDendritic cellM. bovis BCGMajor histocompatibility complex class IIIn vivo |
| spellingShingle | Zhengzhong Xu Aihong Xia Xin Li Zhaocheng Zhu Yechi Shen Shanshan Jin Tian Lan Yuqing Xie Han Wu Chuang Meng Lin Sun Yuelan Yin Xiang Chen Xinan Jiao Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection BMC Immunology Ag-presenting activity Dendritic cell M. bovis BCG Major histocompatibility complex class II In vivo |
| title | Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection |
| title_full | Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection |
| title_fullStr | Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection |
| title_full_unstemmed | Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection |
| title_short | Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection |
| title_sort | rapid loss of early antigen presenting activity of lymph node dendritic cells against ag85a protein following mycobacterium bovis bcg infection |
| topic | Ag-presenting activity Dendritic cell M. bovis BCG Major histocompatibility complex class II In vivo |
| url | http://link.springer.com/article/10.1186/s12865-018-0258-8 |
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