Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers
The research is focused on the prospective representative of the family of biodegradable polymers - polyhydroxyalkanoates (PHAs), the copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB). Difficulties with producing this type of PHA are associated with the fact that the precursor fo...
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| Format: | Article |
| Language: | English |
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Siberian Federal University
2011-06-01
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| Series: | Журнал Сибирского федерального университета: Серия Биология |
| Subjects: | |
| Online Access: | http://elib.sfu-kras.ru/bitstream/2311/2558/1/Zhila.pdf |
| _version_ | 1818309490894700544 |
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| author | Natalia O. Zhila Tatiana G. Volova Elena D. Nikolaeva Daria A. Syrvacheva |
| author_facet | Natalia O. Zhila Tatiana G. Volova Elena D. Nikolaeva Daria A. Syrvacheva |
| author_sort | Natalia O. Zhila |
| collection | DOAJ |
| description | The research is focused on the prospective representative of the family of biodegradable polymers - polyhydroxyalkanoates (PHAs), the copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB). Difficulties with producing this type of PHA are associated with the fact that the precursor for the 4-hydroxybutyrate (4HB) monomers synthesis, the -butyrolactone, is toxic for the bacteria producers and reduces the total biomass production and the polymer yield when it is added to the medium. Having used the natural strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus В10646, the latter possessing the increased sustainability to the -butyrolactone influence, the conditions for efficient synthesis of P(3HB-co-4HB) have been determined. A set of highly purified samples of P(3HB-co-4HB) with different content of 4HB (from 8.7 to 24.3 mol %) was produced. It has been determined that the incorporation of 4HB in the copolymer of a higher degree than 3-hydroxyvalerate and 3-hydroxyhexanoate leads to the decrease of the copolymers crystallinity; samples with the crystallinity of 12 % and 25 % have been obtained. It has been demonstrated that the average molecular weight of the P(3HB-co-4HB) samples and the polydispersity do not depend on the ratio of monomers and vary within the wide range from 540 to 1100 kDa and from 1.91 to 2.76 correspondingly. It has been determined that the 4HB content in the studied range (8.7-24.3 mol %) does not effect the melting temperature of the copolymers (168.9- 172.5 ºC). |
| first_indexed | 2024-12-13T07:31:00Z |
| format | Article |
| id | doaj.art-dd7f495d8e8c4ec49cccec5b992aaa0f |
| institution | Directory Open Access Journal |
| issn | 1997-1389 2313-5530 |
| language | English |
| last_indexed | 2024-12-13T07:31:00Z |
| publishDate | 2011-06-01 |
| publisher | Siberian Federal University |
| record_format | Article |
| series | Журнал Сибирского федерального университета: Серия Биология |
| spelling | doaj.art-dd7f495d8e8c4ec49cccec5b992aaa0f2022-12-21T23:55:13ZengSiberian Federal UniversityЖурнал Сибирского федерального университета: Серия Биология1997-13892313-55302011-06-0142158171http://elib.sfu-kras.ru/handle/2311/2558Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) CopolymersNatalia O. Zhila0Tatiana G. Volova1Elena D. Nikolaeva2Daria A. Syrvacheva3Siberian Federal University Institute of Biophysics Siberian Branch of Russian Academy of Science , 79 Svobodny av., Krasnoyarsk, 660041 Russia 50 Akademgorodok, Krasnoyarsk, 660036 RussiaSiberian Federal University Institute of Biophysics Siberian Branch of Russian Academy of Science , 79 Svobodny av., Krasnoyarsk, 660041 Russia 50 Akademgorodok, Krasnoyarsk, 660036 RussiaSiberian Federal University Institute of Biophysics Siberian Branch of Russian Academy of Science , 79 Svobodny av., Krasnoyarsk, 660041 Russia 50 Akademgorodok, Krasnoyarsk, 660036 RussiaSiberian Federal University , 79 Svobodny av., Krasnoyarsk, 660041 RussiaThe research is focused on the prospective representative of the family of biodegradable polymers - polyhydroxyalkanoates (PHAs), the copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB). Difficulties with producing this type of PHA are associated with the fact that the precursor for the 4-hydroxybutyrate (4HB) monomers synthesis, the -butyrolactone, is toxic for the bacteria producers and reduces the total biomass production and the polymer yield when it is added to the medium. Having used the natural strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus В10646, the latter possessing the increased sustainability to the -butyrolactone influence, the conditions for efficient synthesis of P(3HB-co-4HB) have been determined. A set of highly purified samples of P(3HB-co-4HB) with different content of 4HB (from 8.7 to 24.3 mol %) was produced. It has been determined that the incorporation of 4HB in the copolymer of a higher degree than 3-hydroxyvalerate and 3-hydroxyhexanoate leads to the decrease of the copolymers crystallinity; samples with the crystallinity of 12 % and 25 % have been obtained. It has been demonstrated that the average molecular weight of the P(3HB-co-4HB) samples and the polydispersity do not depend on the ratio of monomers and vary within the wide range from 540 to 1100 kDa and from 1.91 to 2.76 correspondingly. It has been determined that the 4HB content in the studied range (8.7-24.3 mol %) does not effect the melting temperature of the copolymers (168.9- 172.5 ºC).http://elib.sfu-kras.ru/bitstream/2311/2558/1/Zhila.pdfhydrogen-oxidizing bacteriapoly(3-hydroxybutyrate-co-4-hydroxybutyrate)-butyrolactonephysicochemical properties |
| spellingShingle | Natalia O. Zhila Tatiana G. Volova Elena D. Nikolaeva Daria A. Syrvacheva Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers Журнал Сибирского федерального университета: Серия Биология hydrogen-oxidizing bacteria poly(3-hydroxybutyrate-co-4-hydroxybutyrate) -butyrolactone physicochemical properties |
| title | Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers |
| title_full | Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers |
| title_fullStr | Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers |
| title_full_unstemmed | Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers |
| title_short | Microbial Synthesis and Characterization of Poly (3-Hydroxybutyrate-co- 4-Hydroxybutyrate) Copolymers |
| title_sort | microbial synthesis and characterization of poly 3 hydroxybutyrate co 4 hydroxybutyrate copolymers |
| topic | hydrogen-oxidizing bacteria poly(3-hydroxybutyrate-co-4-hydroxybutyrate) -butyrolactone physicochemical properties |
| url | http://elib.sfu-kras.ru/bitstream/2311/2558/1/Zhila.pdf |
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