Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits
Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven r...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2014-02-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100024&lng=en&tlng=en |
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| author | R.R. Filgueiras R.J. Del Carlo N.P. Alves M.I.V. Viloria C.M. McManus M.B. Castro F.P.F. Filgueiras B.S. Monteiro R.M. Almeida R.R. Andrade |
| author_facet | R.R. Filgueiras R.J. Del Carlo N.P. Alves M.I.V. Viloria C.M. McManus M.B. Castro F.P.F. Filgueiras B.S. Monteiro R.M. Almeida R.R. Andrade |
| author_sort | R.R. Filgueiras |
| collection | DOAJ |
| description | Chondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG) received no treatment. The thawed (TH) and fresh (FH) heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP). The CG group showed greatest pain and lameness compared to the other groups seven days after the implantation. Microscopically, at 45 and 90 days, the TH and FH groups showed filling with cartilaginous tissue containing chondrocytes surrounded by a dense matrix of glycosaminoglycans. In the CG group, healing occurred with vascularized fibrous connective tissue without integration to the subchondral bone. Cryopreserved heterologous chondrocytes were viable for implantation and healing of osteochondral lesions; the association with PRP allows the fixation of cells in the lesion and offers growth factors which accelerates repair with tissue similar to articular hyaline cartilage. |
| first_indexed | 2024-12-22T04:22:56Z |
| format | Article |
| id | doaj.art-dd823d2980104899ae85e909975a17c1 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-22T04:22:56Z |
| publishDate | 2014-02-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-dd823d2980104899ae85e909975a17c12022-12-21T18:39:13ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622014-02-0166116817610.1590/S0102-09352014000100024S0102-09352014000100024Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbitsR.R. Filgueiras0R.J. Del Carlo1N.P. AlvesM.I.V. Viloria2C.M. McManus3M.B. Castro4F.P.F. Filgueiras5B.S. Monteiro6R.M. Almeida7R.R. Andrade8União de Ensino Superior de ViçosaUnião de Ensino Superior de ViçosaUnião de Ensino Superior de ViçosaUniversidade Federal de BrasíliaUniversidade Federal de BrasíliaUniversidade Federal de BrasíliaUnião de Ensino Superior de ViçosaUniversidade Federal de BrasíliaUniversidade Federal de BrasíliaChondrocytes obtained from stifle joint of New Zealand White rabbits were cultivated. Half of cells were maintained in culture for later implantation and the others frozen during six months to evaluate viability. A circular osteochondral defect was created in the right stifle of other twenty seven rabbits. The control group (CG) received no treatment. The thawed (TH) and fresh (FH) heterologous groups received, respectively, an implant of cultivated thawed or fresh heterologous chondrocytes associated with platelet rich plasma (PRP). The CG group showed greatest pain and lameness compared to the other groups seven days after the implantation. Microscopically, at 45 and 90 days, the TH and FH groups showed filling with cartilaginous tissue containing chondrocytes surrounded by a dense matrix of glycosaminoglycans. In the CG group, healing occurred with vascularized fibrous connective tissue without integration to the subchondral bone. Cryopreserved heterologous chondrocytes were viable for implantation and healing of osteochondral lesions; the association with PRP allows the fixation of cells in the lesion and offers growth factors which accelerates repair with tissue similar to articular hyaline cartilage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100024&lng=en&tlng=encoelhocartilagem articularcolágeno tipo IIimplante de condrócitosplasma rico em plaquetas |
| spellingShingle | R.R. Filgueiras R.J. Del Carlo N.P. Alves M.I.V. Viloria C.M. McManus M.B. Castro F.P.F. Filgueiras B.S. Monteiro R.M. Almeida R.R. Andrade Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits Arquivo Brasileiro de Medicina Veterinária e Zootecnia coelho cartilagem articular colágeno tipo II implante de condrócitos plasma rico em plaquetas |
| title | Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits |
| title_full | Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits |
| title_fullStr | Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits |
| title_full_unstemmed | Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits |
| title_short | Platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits |
| title_sort | platelet rich plasma associated with heterologous fresh and thawed chondrocytes on osteochondral lesions of rabbits |
| topic | coelho cartilagem articular colágeno tipo II implante de condrócitos plasma rico em plaquetas |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100024&lng=en&tlng=en |
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