VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout
The Viral Hemorrhagic Septicemia Virus (VHSV) is an OIE notifiable pathogen widespread in the Northern Hemisphere that encompasses four genotypes and nine subtypes. In Europe, subtype Ia impairs predominantly the rainbow trout industry causing severe rates of mortality, while other VHSV genotypes an...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01984/full |
| _version_ | 1818408015967027200 |
|---|---|
| author | Valentina Panzarin Argelia Cuenca Michele Gastaldelli Anna L. F. Alencar Francesco Pascoli Thierry Morin Yannick Blanchard Joëlle Cabon Lénaïg Louboutin David Ryder Miriam Abbadi Anna Toffan Carlos P. Dopazo Stéphane Biacchesi Michel Brémont Niels J. Olesen |
| author_facet | Valentina Panzarin Argelia Cuenca Michele Gastaldelli Anna L. F. Alencar Francesco Pascoli Thierry Morin Yannick Blanchard Joëlle Cabon Lénaïg Louboutin David Ryder Miriam Abbadi Anna Toffan Carlos P. Dopazo Stéphane Biacchesi Michel Brémont Niels J. Olesen |
| author_sort | Valentina Panzarin |
| collection | DOAJ |
| description | The Viral Hemorrhagic Septicemia Virus (VHSV) is an OIE notifiable pathogen widespread in the Northern Hemisphere that encompasses four genotypes and nine subtypes. In Europe, subtype Ia impairs predominantly the rainbow trout industry causing severe rates of mortality, while other VHSV genotypes and subtypes affect a number of marine and freshwater species, both farmed and wild. VHSV has repeatedly proved to be able to jump to rainbow trout from the marine reservoir, causing mortality episodes. The molecular mechanisms regulating VHSV virulence and host tropism are not fully understood, mainly due to the scarce availability of complete genome sequences and information on the virulence phenotype. With the scope of identifying in silico molecular markers for VHSV virulence, we generated an extensive dataset of 55 viral genomes and related mortality data obtained from rainbow trout experimental challenges. Using statistical association analyses that combined genetic and mortality data, we found 38 single amino acid polymorphisms scattered throughout the complete coding regions of the viral genome that were putatively involved in virulence of VHSV in trout. Specific amino acid signatures were recognized as being associated with either low or high virulence phenotypes. The phylogenetic analysis of VHSV coding regions supported the evolution toward greater virulence in rainbow trout within subtype Ia, and identified several other subtypes which may be prone to be virulent for this species. This study sheds light on the molecular basis for VHSV virulence, and provides an extensive list of putative virulence markers for their subsequent validation. |
| first_indexed | 2024-12-14T09:37:01Z |
| format | Article |
| id | doaj.art-dd82ae7cda1e4faeb3fc81a939df950b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T09:37:01Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dd82ae7cda1e4faeb3fc81a939df950b2022-12-21T23:07:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-08-011110.3389/fmicb.2020.01984573993VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow TroutValentina Panzarin0Argelia Cuenca1Michele Gastaldelli2Anna L. F. Alencar3Francesco Pascoli4Thierry Morin5Yannick Blanchard6Joëlle Cabon7Lénaïg Louboutin8David Ryder9Miriam Abbadi10Anna Toffan11Carlos P. Dopazo12Stéphane Biacchesi13Michel Brémont14Niels J. Olesen15Division of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Padua, ItalyUnit for Fish and Shellfish Diseases, EURL for Fish and Crustacean Diseases, National Institute of Aquatic Resources, Technical University of Denmark (DTU), Kongens Lyngby, DenmarkDivision of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Padua, ItalyUnit for Fish and Shellfish Diseases, EURL for Fish and Crustacean Diseases, National Institute of Aquatic Resources, Technical University of Denmark (DTU), Kongens Lyngby, DenmarkDivision of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Padua, ItalyUnit of Viral Diseases in Fish, Laboratory of Ploufragan-Plouzané-Niort, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Plouzané, FranceUnit of Viral Genetics and Biosafety, Laboratory of Ploufragan-Plouzané-Niort, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Ploufragan, FranceUnit of Viral Diseases in Fish, Laboratory of Ploufragan-Plouzané-Niort, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Plouzané, FranceUnit of Viral Diseases in Fish, Laboratory of Ploufragan-Plouzané-Niort, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Plouzané, FranceInternational Centre of Excellence for Aquatic Animal Health, CEFAS Weymouth Laboratory, Weymouth, United KingdomDivision of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Padua, ItalyDivision of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Padua, ItalyDepartamento de Microbiología y Parasitología, Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, SpainVirologie et Immunologie Moléculaires, Université Paris-Saclay, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Versailles Saint-Quentin-en-Yvelines, Jouy-en-Josas, FranceVirologie et Immunologie Moléculaires, Université Paris-Saclay, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Versailles Saint-Quentin-en-Yvelines, Jouy-en-Josas, FranceUnit for Fish and Shellfish Diseases, EURL for Fish and Crustacean Diseases, National Institute of Aquatic Resources, Technical University of Denmark (DTU), Kongens Lyngby, DenmarkThe Viral Hemorrhagic Septicemia Virus (VHSV) is an OIE notifiable pathogen widespread in the Northern Hemisphere that encompasses four genotypes and nine subtypes. In Europe, subtype Ia impairs predominantly the rainbow trout industry causing severe rates of mortality, while other VHSV genotypes and subtypes affect a number of marine and freshwater species, both farmed and wild. VHSV has repeatedly proved to be able to jump to rainbow trout from the marine reservoir, causing mortality episodes. The molecular mechanisms regulating VHSV virulence and host tropism are not fully understood, mainly due to the scarce availability of complete genome sequences and information on the virulence phenotype. With the scope of identifying in silico molecular markers for VHSV virulence, we generated an extensive dataset of 55 viral genomes and related mortality data obtained from rainbow trout experimental challenges. Using statistical association analyses that combined genetic and mortality data, we found 38 single amino acid polymorphisms scattered throughout the complete coding regions of the viral genome that were putatively involved in virulence of VHSV in trout. Specific amino acid signatures were recognized as being associated with either low or high virulence phenotypes. The phylogenetic analysis of VHSV coding regions supported the evolution toward greater virulence in rainbow trout within subtype Ia, and identified several other subtypes which may be prone to be virulent for this species. This study sheds light on the molecular basis for VHSV virulence, and provides an extensive list of putative virulence markers for their subsequent validation.https://www.frontiersin.org/article/10.3389/fmicb.2020.01984/fullVHSVmolecular markerssingle amino acid polymorphism (SAP)virulencerainbow trout |
| spellingShingle | Valentina Panzarin Argelia Cuenca Michele Gastaldelli Anna L. F. Alencar Francesco Pascoli Thierry Morin Yannick Blanchard Joëlle Cabon Lénaïg Louboutin David Ryder Miriam Abbadi Anna Toffan Carlos P. Dopazo Stéphane Biacchesi Michel Brémont Niels J. Olesen VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout Frontiers in Microbiology VHSV molecular markers single amino acid polymorphism (SAP) virulence rainbow trout |
| title | VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout |
| title_full | VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout |
| title_fullStr | VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout |
| title_full_unstemmed | VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout |
| title_short | VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout |
| title_sort | vhsv single amino acid polymorphisms saps associated with virulence in rainbow trout |
| topic | VHSV molecular markers single amino acid polymorphism (SAP) virulence rainbow trout |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01984/full |
| work_keys_str_mv | AT valentinapanzarin vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT argeliacuenca vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT michelegastaldelli vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT annalfalencar vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT francescopascoli vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT thierrymorin vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT yannickblanchard vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT joellecabon vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT lenaiglouboutin vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT davidryder vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT miriamabbadi vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT annatoffan vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT carlospdopazo vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT stephanebiacchesi vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT michelbremont vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout AT nielsjolesen vhsvsingleaminoacidpolymorphismssapsassociatedwithvirulenceinrainbowtrout |