WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy
Infectious sequelae caused by surgery are a significant problem in modern medicine due to their reduction of therapeutic effectiveness and the patients’ quality of life.Recently, new methods of local antimicrobial prophylaxis of postoperative sequelae have been actively developed. They allow high lo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/6/1199 |
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| author | Valentina O. Plastun Ekaterina S. Prikhozhdenko Olga I. Gusliakova Svetlana V. Raikova Timothy E. L. Douglas Olga A. Sindeeva Oksana A. Mayorova |
| author_facet | Valentina O. Plastun Ekaterina S. Prikhozhdenko Olga I. Gusliakova Svetlana V. Raikova Timothy E. L. Douglas Olga A. Sindeeva Oksana A. Mayorova |
| author_sort | Valentina O. Plastun |
| collection | DOAJ |
| description | Infectious sequelae caused by surgery are a significant problem in modern medicine due to their reduction of therapeutic effectiveness and the patients’ quality of life.Recently, new methods of local antimicrobial prophylaxis of postoperative sequelae have been actively developed. They allow high local concentrations of drugs to be achieved, increasing the antibiotic therapy’s effectiveness while reducing its side effects. We have developed and characterized antimicrobial hydrogels based on an inexpensive and biocompatible natural substance from the dairy industry—whey protein isolate—as matrices for drug delivery. The release of cefazolin from the pores of hydrogel structures directly depends on the amount of the loaded drug and occurs in a prolonged manner for three days. Simultaneously with the antibiotic release, hydrogel swelling and partial degradation occurs. The WPI hydrogels absorb solvent, doubling in size in three days and retaining cefazolin throughout the duration of the experiment. The antimicrobial activity of cefazolin-loaded WPI hydrogels against <i>Staphylococcus aureus</i> growth is prolonged in comparison to that of the free cefazolin. The overall cytotoxic effect of cefazolin-containing WPI hydrogels is lower than that of free antibiotics. Thus, our work shows that antimicrobial WPI hydrogels are suitable candidates for local antibiotic therapy of infectious surgical sequelae. |
| first_indexed | 2024-03-09T22:45:56Z |
| format | Article |
| id | doaj.art-dd84cb369e3b4b13b87242bf18db97bb |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T22:45:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-dd84cb369e3b4b13b87242bf18db97bb2023-11-23T18:29:51ZengMDPI AGPharmaceutics1999-49232022-06-01146119910.3390/pharmaceutics14061199WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial TherapyValentina O. Plastun0Ekaterina S. Prikhozhdenko1Olga I. Gusliakova2Svetlana V. Raikova3Timothy E. L. Douglas4Olga A. Sindeeva5Oksana A. Mayorova6Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaDepartment of Microbiology, Virology and Immunology, Saratov State Medical University Named after V. I. Razumovsky, 112 Bolshaya Kazachia St., 410012 Saratov, RussiaEngineering Department, Lancaster University, Gillow Av., Lancaster LA1 4YW, UKCenter for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaInfectious sequelae caused by surgery are a significant problem in modern medicine due to their reduction of therapeutic effectiveness and the patients’ quality of life.Recently, new methods of local antimicrobial prophylaxis of postoperative sequelae have been actively developed. They allow high local concentrations of drugs to be achieved, increasing the antibiotic therapy’s effectiveness while reducing its side effects. We have developed and characterized antimicrobial hydrogels based on an inexpensive and biocompatible natural substance from the dairy industry—whey protein isolate—as matrices for drug delivery. The release of cefazolin from the pores of hydrogel structures directly depends on the amount of the loaded drug and occurs in a prolonged manner for three days. Simultaneously with the antibiotic release, hydrogel swelling and partial degradation occurs. The WPI hydrogels absorb solvent, doubling in size in three days and retaining cefazolin throughout the duration of the experiment. The antimicrobial activity of cefazolin-loaded WPI hydrogels against <i>Staphylococcus aureus</i> growth is prolonged in comparison to that of the free cefazolin. The overall cytotoxic effect of cefazolin-containing WPI hydrogels is lower than that of free antibiotics. Thus, our work shows that antimicrobial WPI hydrogels are suitable candidates for local antibiotic therapy of infectious surgical sequelae.https://www.mdpi.com/1999-4923/14/6/1199antimicrobial activityhydrogeldrug releasecefazolinwhey protein isolate |
| spellingShingle | Valentina O. Plastun Ekaterina S. Prikhozhdenko Olga I. Gusliakova Svetlana V. Raikova Timothy E. L. Douglas Olga A. Sindeeva Oksana A. Mayorova WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy Pharmaceutics antimicrobial activity hydrogel drug release cefazolin whey protein isolate |
| title | WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy |
| title_full | WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy |
| title_fullStr | WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy |
| title_full_unstemmed | WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy |
| title_short | WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy |
| title_sort | wpi hydrogels with a prolonged drug release profile for antimicrobial therapy |
| topic | antimicrobial activity hydrogel drug release cefazolin whey protein isolate |
| url | https://www.mdpi.com/1999-4923/14/6/1199 |
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