The Optimization of Short-Term Hepatocyte Preservation Before Transplantation
Background. No optimal methods for short-term hepatocyte preservation have been established. We have recently developed a prominent oxygen-permeable bag (Tohoku Device [TD]) for pancreatic islet culture and transplantation. In this study, we investigated whether TD is also effective for hepatocyte p...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2017-07-01
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| Series: | Transplantation Direct |
| Online Access: | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000000687 |
| _version_ | 1828807037807493120 |
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| author | Kengo Fukuoka, MD Akiko Inagaki, PhD Yasuhiro Nakamura, MD, PhD Muneyuki Matsumura, MD Satoru Yoshida, MD, PhD Takehiro Imura Yasuhiro Igarashi, MD, PhD Shigehito Miyagi, MD, PhD Kazuo Ohashi, MD, PhD Shin Enosawa, MD, PhD Takashi Kamei, MD, PhD Michiaki Unno, MD, PhD Noriaki Ohuchi, MD, PhD Susumu Satomi, MD, PhD Masafumi Goto, MD, PhD |
| author_facet | Kengo Fukuoka, MD Akiko Inagaki, PhD Yasuhiro Nakamura, MD, PhD Muneyuki Matsumura, MD Satoru Yoshida, MD, PhD Takehiro Imura Yasuhiro Igarashi, MD, PhD Shigehito Miyagi, MD, PhD Kazuo Ohashi, MD, PhD Shin Enosawa, MD, PhD Takashi Kamei, MD, PhD Michiaki Unno, MD, PhD Noriaki Ohuchi, MD, PhD Susumu Satomi, MD, PhD Masafumi Goto, MD, PhD |
| author_sort | Kengo Fukuoka, MD |
| collection | DOAJ |
| description | Background. No optimal methods for short-term hepatocyte preservation have been established. We have recently developed a prominent oxygen-permeable bag (Tohoku Device [TD]) for pancreatic islet culture and transplantation. In this study, we investigated whether TD is also effective for hepatocyte preservation and tried to optimize other conditions.
Methods. Hepatocytes were preserved in the following conditions, and their outcomes were observed. First, the effectiveness of TD was investigated. Second, hepatocyte medium (HM) and organ preservation solutions with or without fetal bovine serum (FBS) were compared. Third, as supplementations, FBS and human serum albumin (HSA) were compared. Fourth, low, room and high temperature were compared. And finally, hepatocytes preserved in various conditions were transplanted into the subrenal capsule space of nonalbumin rats and engrafted areas were assessed.
Results. The survival rate of hepatocytes preserved in TD tended to be higher and their viability and function were maintained significantly greater than those of non-TD group. Irrespective of FBS supplementation, the survival rate of HM group was significantly higher than those of organ preservation solution group while viabilities and plating efficiency were similar among them. Although survival rates of groups without FBS were extremely low, results of HSA supplemented group were not inferior to FBS supplemented group. Hepatocytes preserved at high temperature had the worst results. The engrafted area of TD group tended to be higher than those of other groups.
Conclusions. TD is effective for short-term hepatocyte preservation. HSA is a useful substitute for FBS, and preserving in HM at low temperature is recommended. |
| first_indexed | 2024-12-12T08:18:38Z |
| format | Article |
| id | doaj.art-dd866af0c6a04eee8e960daef27ab9a9 |
| institution | Directory Open Access Journal |
| issn | 2373-8731 |
| language | English |
| last_indexed | 2024-12-12T08:18:38Z |
| publishDate | 2017-07-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Transplantation Direct |
| spelling | doaj.art-dd866af0c6a04eee8e960daef27ab9a92022-12-22T00:31:29ZengWolters KluwerTransplantation Direct2373-87312017-07-0137e17610.1097/TXD.0000000000000687201707000-0017The Optimization of Short-Term Hepatocyte Preservation Before TransplantationKengo Fukuoka, MD0Akiko Inagaki, PhD1Yasuhiro Nakamura, MD, PhD2Muneyuki Matsumura, MD3Satoru Yoshida, MD, PhD4Takehiro Imura5Yasuhiro Igarashi, MD, PhD6Shigehito Miyagi, MD, PhD7Kazuo Ohashi, MD, PhD8Shin Enosawa, MD, PhD9Takashi Kamei, MD, PhD10Michiaki Unno, MD, PhD11Noriaki Ohuchi, MD, PhD12Susumu Satomi, MD, PhD13Masafumi Goto, MD, PhD141 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.2 Division of Transplantation and Regenerative Medicine, Tohoku University School of Medicine, Sendai, Japan.3 Division of Pathology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.2 Division of Transplantation and Regenerative Medicine, Tohoku University School of Medicine, Sendai, Japan.2 Division of Transplantation and Regenerative Medicine, Tohoku University School of Medicine, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.4 Laboratory of Drug Development and Science, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.5 Advanced Medical Science, National Center for Child Health and Development, Tokyo, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.1 Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.Background. No optimal methods for short-term hepatocyte preservation have been established. We have recently developed a prominent oxygen-permeable bag (Tohoku Device [TD]) for pancreatic islet culture and transplantation. In this study, we investigated whether TD is also effective for hepatocyte preservation and tried to optimize other conditions. Methods. Hepatocytes were preserved in the following conditions, and their outcomes were observed. First, the effectiveness of TD was investigated. Second, hepatocyte medium (HM) and organ preservation solutions with or without fetal bovine serum (FBS) were compared. Third, as supplementations, FBS and human serum albumin (HSA) were compared. Fourth, low, room and high temperature were compared. And finally, hepatocytes preserved in various conditions were transplanted into the subrenal capsule space of nonalbumin rats and engrafted areas were assessed. Results. The survival rate of hepatocytes preserved in TD tended to be higher and their viability and function were maintained significantly greater than those of non-TD group. Irrespective of FBS supplementation, the survival rate of HM group was significantly higher than those of organ preservation solution group while viabilities and plating efficiency were similar among them. Although survival rates of groups without FBS were extremely low, results of HSA supplemented group were not inferior to FBS supplemented group. Hepatocytes preserved at high temperature had the worst results. The engrafted area of TD group tended to be higher than those of other groups. Conclusions. TD is effective for short-term hepatocyte preservation. HSA is a useful substitute for FBS, and preserving in HM at low temperature is recommended.http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000000687 |
| spellingShingle | Kengo Fukuoka, MD Akiko Inagaki, PhD Yasuhiro Nakamura, MD, PhD Muneyuki Matsumura, MD Satoru Yoshida, MD, PhD Takehiro Imura Yasuhiro Igarashi, MD, PhD Shigehito Miyagi, MD, PhD Kazuo Ohashi, MD, PhD Shin Enosawa, MD, PhD Takashi Kamei, MD, PhD Michiaki Unno, MD, PhD Noriaki Ohuchi, MD, PhD Susumu Satomi, MD, PhD Masafumi Goto, MD, PhD The Optimization of Short-Term Hepatocyte Preservation Before Transplantation Transplantation Direct |
| title | The Optimization of Short-Term Hepatocyte Preservation Before Transplantation |
| title_full | The Optimization of Short-Term Hepatocyte Preservation Before Transplantation |
| title_fullStr | The Optimization of Short-Term Hepatocyte Preservation Before Transplantation |
| title_full_unstemmed | The Optimization of Short-Term Hepatocyte Preservation Before Transplantation |
| title_short | The Optimization of Short-Term Hepatocyte Preservation Before Transplantation |
| title_sort | optimization of short term hepatocyte preservation before transplantation |
| url | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000000687 |
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