Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica
Abstract Background Leaf color variation is a common trait in plants and widely distributed in many plants. In this study, a leaf color mutation in Camellia japonica (cultivar named as Maguxianzi, M) was used as material, and the mechanism of leaf color variation was revealed by physiological, cytol...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-09-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-021-03198-w |
| _version_ | 1818656303253291008 |
|---|---|
| author | Mingyue Fu Zhongcheng Zhou Xu Yang Zhongbing Liu Jiarui Zheng Xinru Huang Ling Wang Jiabao Ye Weiwei Zhang Yongling Liao Feng Xu |
| author_facet | Mingyue Fu Zhongcheng Zhou Xu Yang Zhongbing Liu Jiarui Zheng Xinru Huang Ling Wang Jiabao Ye Weiwei Zhang Yongling Liao Feng Xu |
| author_sort | Mingyue Fu |
| collection | DOAJ |
| description | Abstract Background Leaf color variation is a common trait in plants and widely distributed in many plants. In this study, a leaf color mutation in Camellia japonica (cultivar named as Maguxianzi, M) was used as material, and the mechanism of leaf color variation was revealed by physiological, cytological, transcriptome and microbiome analyses. Results The yellowing C. japonica (M) exhibits lower pigment content than its parent (cultivar named as Huafurong, H), especially chlorophyll (Chl) and carotenoid, and leaves of M have weaker photosynthesis. Subsequently, the results of transmission electron microscopy(TEM) exhibited that M chloroplast was accompanied by broken thylakoid membrane, degraded thylakoid grana, and filled with many vesicles. Furthermore, comparative transcriptome sequencing identified 3,298 differentially expressed genes (DEGs). KEGG annotation analysis results showed that 69 significantly enriched DEGs were involved in Chl biosynthesis, carotenoid biosynthesis, photosynthesis, and plant-pathogen interaction. On this basis, we sequenced the microbial diversity of the H and M leaves. The sequencing results suggested that the abundance of Didymella in the M leaves was significantly higher than that in the H leaves, which meant that M leaves might be infected by Didymella. Conclusions Therefore, we speculated that Didymella infected M leaves while reduced Chl and carotenoid content by damaging chloroplast structures, and altered the intensity of photosynthesis, thereby causing the leaf yellowing phenomenon of C. japonica (M). This research will provide new insights into the leaf color variation mechanism and lay a theoretical foundation for plant breeding and molecular markers. |
| first_indexed | 2024-12-17T03:23:26Z |
| format | Article |
| id | doaj.art-dd86c818f3b24f36a004a601baa74f62 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-17T03:23:26Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-dd86c818f3b24f36a004a601baa74f622022-12-21T22:05:27ZengBMCBMC Plant Biology1471-22292021-09-0121111710.1186/s12870-021-03198-wComparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonicaMingyue Fu0Zhongcheng Zhou1Xu Yang2Zhongbing Liu3Jiarui Zheng4Xinru Huang5Ling Wang6Jiabao Ye7Weiwei Zhang8Yongling Liao9Feng Xu10College of Horticulture and Gardening, Yangtze UniversityDepartment of Forestry Ecology, Hubei Ecology Polytechnic CollegeDepartment of Forestry Ecology, Hubei Ecology Polytechnic CollegeSchool of Horticulture and Landscape, Wuhan University of BioengineeringCollege of Horticulture and Gardening, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityAbstract Background Leaf color variation is a common trait in plants and widely distributed in many plants. In this study, a leaf color mutation in Camellia japonica (cultivar named as Maguxianzi, M) was used as material, and the mechanism of leaf color variation was revealed by physiological, cytological, transcriptome and microbiome analyses. Results The yellowing C. japonica (M) exhibits lower pigment content than its parent (cultivar named as Huafurong, H), especially chlorophyll (Chl) and carotenoid, and leaves of M have weaker photosynthesis. Subsequently, the results of transmission electron microscopy(TEM) exhibited that M chloroplast was accompanied by broken thylakoid membrane, degraded thylakoid grana, and filled with many vesicles. Furthermore, comparative transcriptome sequencing identified 3,298 differentially expressed genes (DEGs). KEGG annotation analysis results showed that 69 significantly enriched DEGs were involved in Chl biosynthesis, carotenoid biosynthesis, photosynthesis, and plant-pathogen interaction. On this basis, we sequenced the microbial diversity of the H and M leaves. The sequencing results suggested that the abundance of Didymella in the M leaves was significantly higher than that in the H leaves, which meant that M leaves might be infected by Didymella. Conclusions Therefore, we speculated that Didymella infected M leaves while reduced Chl and carotenoid content by damaging chloroplast structures, and altered the intensity of photosynthesis, thereby causing the leaf yellowing phenomenon of C. japonica (M). This research will provide new insights into the leaf color variation mechanism and lay a theoretical foundation for plant breeding and molecular markers.https://doi.org/10.1186/s12870-021-03198-wCamellia japonicaLeaf color variationChloroplastTranscriptomeMicrobial diversity |
| spellingShingle | Mingyue Fu Zhongcheng Zhou Xu Yang Zhongbing Liu Jiarui Zheng Xinru Huang Ling Wang Jiabao Ye Weiwei Zhang Yongling Liao Feng Xu Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica BMC Plant Biology Camellia japonica Leaf color variation Chloroplast Transcriptome Microbial diversity |
| title | Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica |
| title_full | Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica |
| title_fullStr | Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica |
| title_full_unstemmed | Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica |
| title_short | Comparative transcriptome and microbial community sequencing provide insight into yellow-leaf phenotype of Camellia japonica |
| title_sort | comparative transcriptome and microbial community sequencing provide insight into yellow leaf phenotype of camellia japonica |
| topic | Camellia japonica Leaf color variation Chloroplast Transcriptome Microbial diversity |
| url | https://doi.org/10.1186/s12870-021-03198-w |
| work_keys_str_mv | AT mingyuefu comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT zhongchengzhou comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT xuyang comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT zhongbingliu comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT jiaruizheng comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT xinruhuang comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT lingwang comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT jiabaoye comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT weiweizhang comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT yonglingliao comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica AT fengxu comparativetranscriptomeandmicrobialcommunitysequencingprovideinsightintoyellowleafphenotypeofcamelliajaponica |