Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway
Abstract Background Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen perox...
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | BMC Molecular and Cell Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12860-022-00435-4 |
| _version_ | 1818018661133189120 |
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| author | Nesa Janamo Berenjabad Vahid Nejati Jafar Rezaie |
| author_facet | Nesa Janamo Berenjabad Vahid Nejati Jafar Rezaie |
| author_sort | Nesa Janamo Berenjabad |
| collection | DOAJ |
| description | Abstract Background Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen peroxide (H2O2), a cellular senescent factor. Results Data showed that the wound healing rate of HUVECs decreased upon incubation with H2O2 (P < 0.05). LOX activity and NO production were decreased in H2O2 treated cells (P < 0.05). Expression of miR-126 and VEGFR-2 up-regulated, while expression of miR-373 and HSP-70 up = regulated in H2O2 -induced cells (P < 0.05). In addition, we found that protein levels of p-Akt-1, VCAM-1, MMP-9, and IL-6 decreased in treated cells (P < 0.05). Conclusions Our data showed that H2O2 reduced the angiogenic response of HUVECs in vitro, which may be due to impairment of the VEGFR-2 signaling pathway. |
| first_indexed | 2024-04-14T07:42:45Z |
| format | Article |
| id | doaj.art-dd86f41ddddd4cd4af9cfc8ad3cb9cbc |
| institution | Directory Open Access Journal |
| issn | 2661-8850 |
| language | English |
| last_indexed | 2024-04-14T07:42:45Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Molecular and Cell Biology |
| spelling | doaj.art-dd86f41ddddd4cd4af9cfc8ad3cb9cbc2022-12-22T02:05:27ZengBMCBMC Molecular and Cell Biology2661-88502022-07-0123111010.1186/s12860-022-00435-4Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathwayNesa Janamo Berenjabad0Vahid Nejati1Jafar Rezaie2Department of Biology, Urmia UniversityDepartment of Biology, Urmia UniversitySolid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical SciencesAbstract Background Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen peroxide (H2O2), a cellular senescent factor. Results Data showed that the wound healing rate of HUVECs decreased upon incubation with H2O2 (P < 0.05). LOX activity and NO production were decreased in H2O2 treated cells (P < 0.05). Expression of miR-126 and VEGFR-2 up-regulated, while expression of miR-373 and HSP-70 up = regulated in H2O2 -induced cells (P < 0.05). In addition, we found that protein levels of p-Akt-1, VCAM-1, MMP-9, and IL-6 decreased in treated cells (P < 0.05). Conclusions Our data showed that H2O2 reduced the angiogenic response of HUVECs in vitro, which may be due to impairment of the VEGFR-2 signaling pathway.https://doi.org/10.1186/s12860-022-00435-4AngiogenesisHUVECsH2O2Age-related diseasesSenescence |
| spellingShingle | Nesa Janamo Berenjabad Vahid Nejati Jafar Rezaie Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway BMC Molecular and Cell Biology Angiogenesis HUVECs H2O2 Age-related diseases Senescence |
| title | Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway |
| title_full | Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway |
| title_fullStr | Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway |
| title_full_unstemmed | Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway |
| title_short | Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway |
| title_sort | angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide possible role of vegfr 2 akt 1 signaling pathway |
| topic | Angiogenesis HUVECs H2O2 Age-related diseases Senescence |
| url | https://doi.org/10.1186/s12860-022-00435-4 |
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