DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card
Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive strategy in order to maintain the genetic variation of each individual and avoid inbreeding drive...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Syiah Kuala University
2018-05-01
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| Series: | International Journal of Tropical Veterinary and Biomedical Research |
| Subjects: | |
| Online Access: | https://jurnal.usk.ac.id/IJTVBR/article/view/11368 |
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| author | Elly L. Rustiati Priyambodo Priyambodo Siti Asiyah Dedi Candra Diah E. Anggraini Elizabeth D. Krismuniarti Eko Agus Srihanto Liza Angeliya Nuning Nurcahyani Enny Saswiyanti |
| author_facet | Elly L. Rustiati Priyambodo Priyambodo Siti Asiyah Dedi Candra Diah E. Anggraini Elizabeth D. Krismuniarti Eko Agus Srihanto Liza Angeliya Nuning Nurcahyani Enny Saswiyanti |
| author_sort | Elly L. Rustiati |
| collection | DOAJ |
| description | Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant
mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive
strategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, genetic
studies have opened new field studies in ecology, included conservation ecology. Patterns in variation of population
has been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’s
ID Card is a necessary in database building, which included morphology, health status, and genetic profile. Genetic
profile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNA
isolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captive
sumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oC
and isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplification
analysis. DNA isolation was successfully done, for further individual genetic ID building. |
| first_indexed | 2024-03-12T23:50:46Z |
| format | Article |
| id | doaj.art-dd890ebd68f44dfb877eece0f2cd7e7c |
| institution | Directory Open Access Journal |
| issn | 2503-4715 |
| language | English |
| last_indexed | 2024-03-12T23:50:46Z |
| publishDate | 2018-05-01 |
| publisher | Syiah Kuala University |
| record_format | Article |
| series | International Journal of Tropical Veterinary and Biomedical Research |
| spelling | doaj.art-dd890ebd68f44dfb877eece0f2cd7e7c2023-07-13T16:07:28ZengSyiah Kuala UniversityInternational Journal of Tropical Veterinary and Biomedical Research2503-47152018-05-01311310.21157/ijtvbr.v3i1.113688630DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID CardElly L. RustiatiPriyambodo PriyambodoSiti AsiyahDedi CandraDiah E. AnggrainiElizabeth D. KrismuniartiEko Agus SrihantoLiza AngeliyaNuning NurcahyaniEnny SaswiyantiElephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive strategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, genetic studies have opened new field studies in ecology, included conservation ecology. Patterns in variation of population has been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’s ID Card is a necessary in database building, which included morphology, health status, and genetic profile. Genetic profile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNA isolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captive sumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oC and isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplification analysis. DNA isolation was successfully done, for further individual genetic ID building.https://jurnal.usk.ac.id/IJTVBR/article/view/11368conservationdna isolationsumatran elephant’s id cardwknp |
| spellingShingle | Elly L. Rustiati Priyambodo Priyambodo Siti Asiyah Dedi Candra Diah E. Anggraini Elizabeth D. Krismuniarti Eko Agus Srihanto Liza Angeliya Nuning Nurcahyani Enny Saswiyanti DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card International Journal of Tropical Veterinary and Biomedical Research conservation dna isolation sumatran elephant’s id card wknp |
| title | DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card |
| title_full | DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card |
| title_fullStr | DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card |
| title_full_unstemmed | DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card |
| title_short | DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card |
| title_sort | dna isolation on captive sumatran elephant in elephant training center way kambas national park a first step towards its id card |
| topic | conservation dna isolation sumatran elephant’s id card wknp |
| url | https://jurnal.usk.ac.id/IJTVBR/article/view/11368 |
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