Chemical composition and antibacterial properties of Peganum harmala L.
<strong>Objective: </strong>The present study was conducted to investigate antibacterial properties of fruit and flower of <em>Peganum harmala</em>.<br /> <strong>Material and Methods: </strong>Column chromatography, followed by preparative thin layer chroma...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2019-10-01
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| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | http://ajp.mums.ac.ir/article_13382_5a95427b2e7bd8cd65894f63255782d0.pdf |
| _version_ | 1818320433545478144 |
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| author | Milad Iranshahy Sedigheh Fazli Bazaz Giti Haririzadeh Bibi Zahra Abootorabi Ali Mohammad Mohamadi Zahra khashyarmanesh |
| author_facet | Milad Iranshahy Sedigheh Fazli Bazaz Giti Haririzadeh Bibi Zahra Abootorabi Ali Mohammad Mohamadi Zahra khashyarmanesh |
| author_sort | Milad Iranshahy |
| collection | DOAJ |
| description | <strong>Objective: </strong>The present study was conducted to investigate antibacterial properties of fruit and flower of <em>Peganum harmala</em>.<br /> <strong>Material and Methods: </strong>Column chromatography, followed by preparative thin layer chromatography (TLC) was used for final purification. The structure of pure alkaloids was determined using spectroscopic methods (<sup>1</sup>H-NMR, <sup>13</sup>C-NMR, UV and MS). Smoke and extract of total alkaloids were investigated for antimicrobial activity against five different microorganisms (standards and hospital isolates). The antibacterial activity was evaluated using disc diffusion assay and minimum inhibitory concentration (MIC) was determined by serial dilution methods.<br /> <strong>Results:</strong> Chemical investigation of the chloroform extract of ripe fruit and flower of <em>P. harmala</em> led to identification of three alkaloids in ripe fruit and two alkaloids in the flower and leaves of this plant. Alkaloids identified in ripe fruit were harmine, peganine (vasicine) and harmaline. Two alkaloids, harmine and peganine, were detected in the flower of <em>P. harmala, </em>while harmaline was only found in the ripe fruit. The total alkaloids of flower were compared with total alkaloids of ripe fruit by TLC method. Fruits and flowers had 3.12 and 3.27% alkaloid contents, respectively.<br /> <strong>Conclusion: </strong>Our results showed that the alkaloids and smoke were specifically more effective on <em>Candida albicans</em> and Gram- positive bacteria (<em>Micrococcus luteus</em> and <em>Staphylococcus aureus</em>), while Gram- negative bacteria, especially <em>Pseudomonas aeruginosa</em>,were less sensitive. |
| first_indexed | 2024-12-13T10:24:56Z |
| format | Article |
| id | doaj.art-dd894ea62f074a8eb4253902002dafe2 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-13T10:24:56Z |
| publishDate | 2019-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-dd894ea62f074a8eb4253902002dafe22022-12-21T23:51:05ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492019-10-019653053710.22038/ajp.2019.1338213382Chemical composition and antibacterial properties of Peganum harmala L.Milad Iranshahy0Sedigheh Fazli Bazaz1Giti Haririzadeh2Bibi Zahra Abootorabi3Ali Mohammad Mohamadi4Zahra khashyarmanesh5Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.|Pharmaceutical Control Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranMedicinal Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Control Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranMedicinal Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.<strong>Objective: </strong>The present study was conducted to investigate antibacterial properties of fruit and flower of <em>Peganum harmala</em>.<br /> <strong>Material and Methods: </strong>Column chromatography, followed by preparative thin layer chromatography (TLC) was used for final purification. The structure of pure alkaloids was determined using spectroscopic methods (<sup>1</sup>H-NMR, <sup>13</sup>C-NMR, UV and MS). Smoke and extract of total alkaloids were investigated for antimicrobial activity against five different microorganisms (standards and hospital isolates). The antibacterial activity was evaluated using disc diffusion assay and minimum inhibitory concentration (MIC) was determined by serial dilution methods.<br /> <strong>Results:</strong> Chemical investigation of the chloroform extract of ripe fruit and flower of <em>P. harmala</em> led to identification of three alkaloids in ripe fruit and two alkaloids in the flower and leaves of this plant. Alkaloids identified in ripe fruit were harmine, peganine (vasicine) and harmaline. Two alkaloids, harmine and peganine, were detected in the flower of <em>P. harmala, </em>while harmaline was only found in the ripe fruit. The total alkaloids of flower were compared with total alkaloids of ripe fruit by TLC method. Fruits and flowers had 3.12 and 3.27% alkaloid contents, respectively.<br /> <strong>Conclusion: </strong>Our results showed that the alkaloids and smoke were specifically more effective on <em>Candida albicans</em> and Gram- positive bacteria (<em>Micrococcus luteus</em> and <em>Staphylococcus aureus</em>), while Gram- negative bacteria, especially <em>Pseudomonas aeruginosa</em>,were less sensitive.http://ajp.mums.ac.ir/article_13382_5a95427b2e7bd8cd65894f63255782d0.pdfAlkaloidAntimicrobial activityPeganum harmalaHarmineHarmalineSmoke |
| spellingShingle | Milad Iranshahy Sedigheh Fazli Bazaz Giti Haririzadeh Bibi Zahra Abootorabi Ali Mohammad Mohamadi Zahra khashyarmanesh Chemical composition and antibacterial properties of Peganum harmala L. Avicenna Journal of Phytomedicine Alkaloid Antimicrobial activity Peganum harmala Harmine Harmaline Smoke |
| title | Chemical composition and antibacterial properties of Peganum harmala L. |
| title_full | Chemical composition and antibacterial properties of Peganum harmala L. |
| title_fullStr | Chemical composition and antibacterial properties of Peganum harmala L. |
| title_full_unstemmed | Chemical composition and antibacterial properties of Peganum harmala L. |
| title_short | Chemical composition and antibacterial properties of Peganum harmala L. |
| title_sort | chemical composition and antibacterial properties of peganum harmala l |
| topic | Alkaloid Antimicrobial activity Peganum harmala Harmine Harmaline Smoke |
| url | http://ajp.mums.ac.ir/article_13382_5a95427b2e7bd8cd65894f63255782d0.pdf |
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