SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro
Yang Xue, Qingqing Chen, Tingting Ding, Jiao SunShanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of ChinaAbstract: The liver has been sh...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2014-06-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/sio2-nanoparticle-induced-impairment-of-mitochondrial-energy-metabolis-a17167 |
| _version_ | 1819004232421867520 |
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| author | Xue Y Chen Q Ding T Sun J |
| author_facet | Xue Y Chen Q Ding T Sun J |
| author_sort | Xue Y |
| collection | DOAJ |
| description | Yang Xue, Qingqing Chen, Tingting Ding, Jiao SunShanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of ChinaAbstract: The liver has been shown to be a primary target organ for SiO2 nanoparticles in vivo, and may be highly susceptible to damage by these nanoparticles. However, until now, research focusing on the potential toxic effects of SiO2 nanoparticles on mitochondria-associated energy metabolism in hepatocytes has been lacking. In this work, SiO2 nanoparticles 20 nm in diameter were evaluated for their ability to induce dysfunction of mitochondrial energy metabolism. First, a buffalo rat liver (BRL) cell line was directly exposed to SiO2 nanoparticles, which induced cytotoxicity and mitochondrial damage accompanied by decreases in mitochondrial dehydrogenase activity, mitochondrial membrane potential, enzymatic expression in the Krebs cycle, and activity of the mitochondrial respiratory chain complexes I, III and IV. Second, the role of rat-derived Kupffer cells was evaluated. The supernatants from Kupffer cells treated with SiO2 nanoparticles were transferred to stimulate BRL cells. We observed that SiO2 nanoparticles had the ability to activate Kupffer cells, leading to release of tumor necrosis factor-α, nitric oxide, and reactive oxygen species from these cells and subsequently to inhibition of mitochondrial respiratory chain complex I activity in BRL cells.Keywords: SiO2 nanoparticles, mitochondria-associated energy metabolism, buffalo rat liver cells, Kupffer cells |
| first_indexed | 2024-12-20T23:33:37Z |
| format | Article |
| id | doaj.art-dd8ad344c7a947b89adb4545c9687263 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-20T23:33:37Z |
| publishDate | 2014-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-dd8ad344c7a947b89adb4545c96872632022-12-21T19:23:15ZengDove Medical PressInternational Journal of Nanomedicine1178-20132014-06-012014Issue 12891290317167SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitroXue YChen QDing TSun JYang Xue, Qingqing Chen, Tingting Ding, Jiao SunShanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of ChinaAbstract: The liver has been shown to be a primary target organ for SiO2 nanoparticles in vivo, and may be highly susceptible to damage by these nanoparticles. However, until now, research focusing on the potential toxic effects of SiO2 nanoparticles on mitochondria-associated energy metabolism in hepatocytes has been lacking. In this work, SiO2 nanoparticles 20 nm in diameter were evaluated for their ability to induce dysfunction of mitochondrial energy metabolism. First, a buffalo rat liver (BRL) cell line was directly exposed to SiO2 nanoparticles, which induced cytotoxicity and mitochondrial damage accompanied by decreases in mitochondrial dehydrogenase activity, mitochondrial membrane potential, enzymatic expression in the Krebs cycle, and activity of the mitochondrial respiratory chain complexes I, III and IV. Second, the role of rat-derived Kupffer cells was evaluated. The supernatants from Kupffer cells treated with SiO2 nanoparticles were transferred to stimulate BRL cells. We observed that SiO2 nanoparticles had the ability to activate Kupffer cells, leading to release of tumor necrosis factor-α, nitric oxide, and reactive oxygen species from these cells and subsequently to inhibition of mitochondrial respiratory chain complex I activity in BRL cells.Keywords: SiO2 nanoparticles, mitochondria-associated energy metabolism, buffalo rat liver cells, Kupffer cellshttp://www.dovepress.com/sio2-nanoparticle-induced-impairment-of-mitochondrial-energy-metabolis-a17167 |
| spellingShingle | Xue Y Chen Q Ding T Sun J SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro International Journal of Nanomedicine |
| title | SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro |
| title_full | SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro |
| title_fullStr | SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro |
| title_full_unstemmed | SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro |
| title_short | SiO2 nanoparticle-induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a Kupffer cell-mediated pathway in vitro |
| title_sort | sio2 nanoparticle induced impairment of mitochondrial energy metabolism in hepatocytes directly and through a kupffer cell mediated pathway in vitro |
| url | http://www.dovepress.com/sio2-nanoparticle-induced-impairment-of-mitochondrial-energy-metabolis-a17167 |
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