Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot
IntroductionSugarcane is one of the most important sugar crops worldwide, however, sugarcane production is seriously limited by sugarcane red rot, a soil-borne disease caused by Colletotrichum falcatum. Bacillus velezensis YC89 was isolated from sugarcane leaves and can significantly inhibited red r...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1180474/full |
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| author | Linyan Xie Lufeng Liu Yanju Luo Xibing Rao Yining Di Yining Di Han Liu Zhenfeng Qian Qingqing Shen Lilian He Lilian He Fusheng Li Fusheng Li |
| author_facet | Linyan Xie Lufeng Liu Yanju Luo Xibing Rao Yining Di Yining Di Han Liu Zhenfeng Qian Qingqing Shen Lilian He Lilian He Fusheng Li Fusheng Li |
| author_sort | Linyan Xie |
| collection | DOAJ |
| description | IntroductionSugarcane is one of the most important sugar crops worldwide, however, sugarcane production is seriously limited by sugarcane red rot, a soil-borne disease caused by Colletotrichum falcatum. Bacillus velezensis YC89 was isolated from sugarcane leaves and can significantly inhibited red rot disease caused by C. falcatum.MethodsIn this study, the genome of YC89 strain was sequenced, its genome structure and function were analyzed using various bioinformatics software, and its genome was compared with those of other homologous strains. In addition, the effectiveness of YC89 against sugarcane red rot and the evaluation of sugarcane plant growth promotion were also investigated by pot experiments.ResultsHere, we present the complete genome sequence of YC89, which consists of a 3.95 Mb circular chromosome with an average GC content of 46.62%. The phylogenetic tree indicated that YC89 is closely related to B. velezensis GS-1. Comparative genome analysis of YC89 with other published strains (B. velezensis FZB42, B. velezensis CC09, B. velezensis SQR9, B. velezensis GS-1, and B. amyloliquefaciens DSM7) revealed that the strains had a part common coding sequences (CDS) in whereas 42 coding were unique of strain YC89. Whole-genome sequencing revealed 547 carbohydrate-active enzymes and identified 12 gene clusters encoding secondary metabolites. Additionally, functional analysis of the genome revealed numerous gene/gene clusters involved in plant growth promotion, antibiotic resistance, and resistance inducer synthesis. In vitro pot tests indicated that YC89 strain controlled sugarcane red rot and promoted the growth of sugarcane plants. Additionally, it increased the activity of enzymes involved in plant defense, such as superoxide dismutase, peroxidase, polyphenol oxidase, chitinase, and β-1,3-glucanase.DiscussionThese findings will be helpful for further studies on the mechanisms of plant growth promotion and biocontrol by B. velezensis and provide an effective strategy for controlling red rot in sugarcane plants. |
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| language | English |
| last_indexed | 2024-03-13T00:17:49Z |
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| spelling | doaj.art-dd8b91b30e514bca987923dc237341742023-07-11T19:52:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11804741180474Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rotLinyan Xie0Lufeng Liu1Yanju Luo2Xibing Rao3Yining Di4Yining Di5Han Liu6Zhenfeng Qian7Qingqing Shen8Lilian He9Lilian He10Fusheng Li11Fusheng Li12College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaSugarcane Research Institute, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, ChinaCollege of Resources and Environment, Yunnan Agricultural University, Kunming, ChinaIntroductionSugarcane is one of the most important sugar crops worldwide, however, sugarcane production is seriously limited by sugarcane red rot, a soil-borne disease caused by Colletotrichum falcatum. Bacillus velezensis YC89 was isolated from sugarcane leaves and can significantly inhibited red rot disease caused by C. falcatum.MethodsIn this study, the genome of YC89 strain was sequenced, its genome structure and function were analyzed using various bioinformatics software, and its genome was compared with those of other homologous strains. In addition, the effectiveness of YC89 against sugarcane red rot and the evaluation of sugarcane plant growth promotion were also investigated by pot experiments.ResultsHere, we present the complete genome sequence of YC89, which consists of a 3.95 Mb circular chromosome with an average GC content of 46.62%. The phylogenetic tree indicated that YC89 is closely related to B. velezensis GS-1. Comparative genome analysis of YC89 with other published strains (B. velezensis FZB42, B. velezensis CC09, B. velezensis SQR9, B. velezensis GS-1, and B. amyloliquefaciens DSM7) revealed that the strains had a part common coding sequences (CDS) in whereas 42 coding were unique of strain YC89. Whole-genome sequencing revealed 547 carbohydrate-active enzymes and identified 12 gene clusters encoding secondary metabolites. Additionally, functional analysis of the genome revealed numerous gene/gene clusters involved in plant growth promotion, antibiotic resistance, and resistance inducer synthesis. In vitro pot tests indicated that YC89 strain controlled sugarcane red rot and promoted the growth of sugarcane plants. Additionally, it increased the activity of enzymes involved in plant defense, such as superoxide dismutase, peroxidase, polyphenol oxidase, chitinase, and β-1,3-glucanase.DiscussionThese findings will be helpful for further studies on the mechanisms of plant growth promotion and biocontrol by B. velezensis and provide an effective strategy for controlling red rot in sugarcane plants.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1180474/fullBacillus velezensisbiocontrolsugarcane red rotgenome sequencingbiocontrol mechanism |
| spellingShingle | Linyan Xie Lufeng Liu Yanju Luo Xibing Rao Yining Di Yining Di Han Liu Zhenfeng Qian Qingqing Shen Lilian He Lilian He Fusheng Li Fusheng Li Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot Frontiers in Microbiology Bacillus velezensis biocontrol sugarcane red rot genome sequencing biocontrol mechanism |
| title | Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot |
| title_full | Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot |
| title_fullStr | Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot |
| title_full_unstemmed | Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot |
| title_short | Complete genome sequence of biocontrol strain Bacillus velezensis YC89 and its biocontrol potential against sugarcane red rot |
| title_sort | complete genome sequence of biocontrol strain bacillus velezensis yc89 and its biocontrol potential against sugarcane red rot |
| topic | Bacillus velezensis biocontrol sugarcane red rot genome sequencing biocontrol mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1180474/full |
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