Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to corre...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-01-01
|
| Series: | Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009 |
| _version_ | 1818128552491483136 |
|---|---|
| author | PATRICIA LODATO JENNIFER ALCAÍNO SALVADOR BARAHONA PATRICIO RETAMALES ANTONIO JIMÉNEZ VÍCTOR CIFUENTES |
| author_facet | PATRICIA LODATO JENNIFER ALCAÍNO SALVADOR BARAHONA PATRICIO RETAMALES ANTONIO JIMÉNEZ VÍCTOR CIFUENTES |
| author_sort | PATRICIA LODATO |
| collection | DOAJ |
| description | The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was not correlated with the transcript level of carotenogenic genes under the same experimental conditions. This result suggests that there is not a linear relationship between carotenogenic transcript levels and carotenoid biosynthesis. |
| first_indexed | 2024-12-11T07:35:04Z |
| format | Article |
| id | doaj.art-dd8cbfb55e324817a627227dbc7788c2 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-11T07:35:04Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-dd8cbfb55e324817a627227dbc7788c22022-12-22T01:15:44ZengBMCBiological Research0716-97600717-62872004-01-013718393Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)PATRICIA LODATOJENNIFER ALCAÍNOSALVADOR BARAHONAPATRICIO RETAMALESANTONIO JIMÉNEZVÍCTOR CIFUENTESThe expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was not correlated with the transcript level of carotenogenic genes under the same experimental conditions. This result suggests that there is not a linear relationship between carotenogenic transcript levels and carotenoid biosynthesis.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009Carotenoid biosynthesisoverproducer strainsXanthophyllomyces dendrorhousPhaffia rhodozyma |
| spellingShingle | PATRICIA LODATO JENNIFER ALCAÍNO SALVADOR BARAHONA PATRICIO RETAMALES ANTONIO JIMÉNEZ VÍCTOR CIFUENTES Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma) Biological Research Carotenoid biosynthesis overproducer strains Xanthophyllomyces dendrorhous Phaffia rhodozyma |
| title | Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma) |
| title_full | Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma) |
| title_fullStr | Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma) |
| title_full_unstemmed | Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma) |
| title_short | Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma) |
| title_sort | study of the expression of carotenoid biosynthesis genes in wild type and deregulated strains of xanthophyllomyces dendrorhous ex phaffia rhodozyma |
| topic | Carotenoid biosynthesis overproducer strains Xanthophyllomyces dendrorhous Phaffia rhodozyma |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009 |
| work_keys_str_mv | AT patricialodato studyoftheexpressionofcarotenoidbiosynthesisgenesinwildtypeandderegulatedstrainsofxanthophyllomycesdendrorhousexphaffiarhodozyma AT jenniferalcaino studyoftheexpressionofcarotenoidbiosynthesisgenesinwildtypeandderegulatedstrainsofxanthophyllomycesdendrorhousexphaffiarhodozyma AT salvadorbarahona studyoftheexpressionofcarotenoidbiosynthesisgenesinwildtypeandderegulatedstrainsofxanthophyllomycesdendrorhousexphaffiarhodozyma AT patricioretamales studyoftheexpressionofcarotenoidbiosynthesisgenesinwildtypeandderegulatedstrainsofxanthophyllomycesdendrorhousexphaffiarhodozyma AT antoniojimenez studyoftheexpressionofcarotenoidbiosynthesisgenesinwildtypeandderegulatedstrainsofxanthophyllomycesdendrorhousexphaffiarhodozyma AT victorcifuentes studyoftheexpressionofcarotenoidbiosynthesisgenesinwildtypeandderegulatedstrainsofxanthophyllomycesdendrorhousexphaffiarhodozyma |