Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions
Summary: To address the question of why more than one myosin-II isoform is expressed in a single cell to drive cytokinesis, we analyzed the roles of the myosin-II isoforms, Myo2 and Myp2, of the fission yeast Schizosaccharomyces pombe, in cytokinesis under normal and stressed conditions. We found th...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-04-01
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| Series: | iScience |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900421930080X |
| _version_ | 1819276916658536448 |
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| author | Hiroki Okada Carsten Wloka Jian-Qiu Wu Erfei Bi |
| author_facet | Hiroki Okada Carsten Wloka Jian-Qiu Wu Erfei Bi |
| author_sort | Hiroki Okada |
| collection | DOAJ |
| description | Summary: To address the question of why more than one myosin-II isoform is expressed in a single cell to drive cytokinesis, we analyzed the roles of the myosin-II isoforms, Myo2 and Myp2, of the fission yeast Schizosaccharomyces pombe, in cytokinesis under normal and stressed conditions. We found that Myp2 controls the disassembly, stability, and constriction initiation of the Myo2 ring in response to high-salt stress. A C-terminal coiled-coil domain of Myp2 is required for its immobility and contractility during cytokinesis, and when fused to the tail of the dynamic Myo2, renders the chimera the low-turnover property. We also found, by following distinct processes in real time at the single-cell level, that Myo2 and Myp2 are differentially required but collectively essential for guiding extracellular matrix remodeling during cytokinesis. These results suggest that the dynamic and immobile myosin-II isoforms are evolved to carry out cytokinesis with robustness under different growth conditions. : Molecular Mechanism of Behavior; Cell Biology; Functional Aspects of Cell Biology Subject Areas: Molecular Mechanism of Behavior, Cell Biology, Functional Aspects of Cell Biology |
| first_indexed | 2024-12-23T23:47:49Z |
| format | Article |
| id | doaj.art-dd8e9af01f6b490f95ce583ae79f672c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-23T23:47:49Z |
| publishDate | 2019-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-dd8e9af01f6b490f95ce583ae79f672c2022-12-21T17:25:28ZengElsevieriScience2589-00422019-04-01146987Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed ConditionsHiroki Okada0Carsten Wloka1Jian-Qiu Wu2Erfei Bi3Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USADepartment of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USA; Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AE Groningen, The NetherlandsDepartment of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USADepartment of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USA; Corresponding authorSummary: To address the question of why more than one myosin-II isoform is expressed in a single cell to drive cytokinesis, we analyzed the roles of the myosin-II isoforms, Myo2 and Myp2, of the fission yeast Schizosaccharomyces pombe, in cytokinesis under normal and stressed conditions. We found that Myp2 controls the disassembly, stability, and constriction initiation of the Myo2 ring in response to high-salt stress. A C-terminal coiled-coil domain of Myp2 is required for its immobility and contractility during cytokinesis, and when fused to the tail of the dynamic Myo2, renders the chimera the low-turnover property. We also found, by following distinct processes in real time at the single-cell level, that Myo2 and Myp2 are differentially required but collectively essential for guiding extracellular matrix remodeling during cytokinesis. These results suggest that the dynamic and immobile myosin-II isoforms are evolved to carry out cytokinesis with robustness under different growth conditions. : Molecular Mechanism of Behavior; Cell Biology; Functional Aspects of Cell Biology Subject Areas: Molecular Mechanism of Behavior, Cell Biology, Functional Aspects of Cell Biologyhttp://www.sciencedirect.com/science/article/pii/S258900421930080X |
| spellingShingle | Hiroki Okada Carsten Wloka Jian-Qiu Wu Erfei Bi Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions iScience |
| title | Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions |
| title_full | Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions |
| title_fullStr | Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions |
| title_full_unstemmed | Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions |
| title_short | Distinct Roles of Myosin-II Isoforms in Cytokinesis under Normal and Stressed Conditions |
| title_sort | distinct roles of myosin ii isoforms in cytokinesis under normal and stressed conditions |
| url | http://www.sciencedirect.com/science/article/pii/S258900421930080X |
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