Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i>
<i>Fusarium verticillioides</i> is the major maize pathogen associated with ear rot and stalk rot worldwide. Fumonisin B1 (FB1) produced by <i>F. verticillioides</i>, poses a serious threat to human and animal health. However, our understanding of FB1 synthesis and virulence...
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MDPI AG
2021-10-01
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| author | Qi Deng Hanxiang Wu Qin Gu Guangfei Tang Wende Liu |
| author_facet | Qi Deng Hanxiang Wu Qin Gu Guangfei Tang Wende Liu |
| author_sort | Qi Deng |
| collection | DOAJ |
| description | <i>Fusarium verticillioides</i> is the major maize pathogen associated with ear rot and stalk rot worldwide. Fumonisin B1 (FB1) produced by <i>F. verticillioides</i>, poses a serious threat to human and animal health. However, our understanding of FB1 synthesis and virulence mechanism in this fungus is still very limited. Glycosylation catalyzed by glycosyltransferases (GTs) has been identified as contributing to fungal infection and secondary metabolism synthesis. In this study, a family 2 glycosyltransferase, FvCpsA, was identified and characterized in <i>F. verticillioides</i>. Δ<i>FvcpsA</i> exhibited significant defects in vegetative growth. Moreover, Δ<i>FvcpsA</i> also increased resistance to osmotic and cell wall stress agents. In addition, expression levels of <i>FUM</i> genes involved in FB1 production were greatly up-regulated in Δ<i>FvcpsA</i>. HPLC (high performance liquid chromatography) analysis revealed that Δ<i>FvcpsA</i> significantly increased FB1 production. Interestingly, we found that the deletion of <i>FvCPSA</i> showed penetration defects on cellophane membrane, and thus led to obvious defects in pathogenicity. Characterization of FvCpsA domain experiments showed that conserved DXD and QXXRW domains were vital for the biological functions of FvCpsA. Taken together, our results indicate that FvCpsA is critical for fungal growth, FB1 biosynthesis and virulence in <i>F. verticillioides</i>. |
| first_indexed | 2024-03-10T06:09:55Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T06:09:55Z |
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| series | Toxins |
| spelling | doaj.art-dd9843c498eb477485683fd9b1ba8a412023-11-22T20:13:23ZengMDPI AGToxins2072-66512021-10-01131071810.3390/toxins13100718Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i>Qi Deng0Hanxiang Wu1Qin Gu2Guangfei Tang3Wende Liu4State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China<i>Fusarium verticillioides</i> is the major maize pathogen associated with ear rot and stalk rot worldwide. Fumonisin B1 (FB1) produced by <i>F. verticillioides</i>, poses a serious threat to human and animal health. However, our understanding of FB1 synthesis and virulence mechanism in this fungus is still very limited. Glycosylation catalyzed by glycosyltransferases (GTs) has been identified as contributing to fungal infection and secondary metabolism synthesis. In this study, a family 2 glycosyltransferase, FvCpsA, was identified and characterized in <i>F. verticillioides</i>. Δ<i>FvcpsA</i> exhibited significant defects in vegetative growth. Moreover, Δ<i>FvcpsA</i> also increased resistance to osmotic and cell wall stress agents. In addition, expression levels of <i>FUM</i> genes involved in FB1 production were greatly up-regulated in Δ<i>FvcpsA</i>. HPLC (high performance liquid chromatography) analysis revealed that Δ<i>FvcpsA</i> significantly increased FB1 production. Interestingly, we found that the deletion of <i>FvCPSA</i> showed penetration defects on cellophane membrane, and thus led to obvious defects in pathogenicity. Characterization of FvCpsA domain experiments showed that conserved DXD and QXXRW domains were vital for the biological functions of FvCpsA. Taken together, our results indicate that FvCpsA is critical for fungal growth, FB1 biosynthesis and virulence in <i>F. verticillioides</i>.https://www.mdpi.com/2072-6651/13/10/718<i>Fusarium verticillioides</i>family 2 glycosyltransferase (GT2)fumonisin B1virulence |
| spellingShingle | Qi Deng Hanxiang Wu Qin Gu Guangfei Tang Wende Liu Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i> Toxins <i>Fusarium verticillioides</i> family 2 glycosyltransferase (GT2) fumonisin B1 virulence |
| title | Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i> |
| title_full | Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i> |
| title_fullStr | Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i> |
| title_full_unstemmed | Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i> |
| title_short | Glycosyltransferase FvCpsA Regulates Fumonisin Biosynthesis and Virulence in <i>Fusarium verticillioides</i> |
| title_sort | glycosyltransferase fvcpsa regulates fumonisin biosynthesis and virulence in i fusarium verticillioides i |
| topic | <i>Fusarium verticillioides</i> family 2 glycosyltransferase (GT2) fumonisin B1 virulence |
| url | https://www.mdpi.com/2072-6651/13/10/718 |
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