Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa
Leila Ahmadian,1,2 Mohammad Reza Haghshenas,1,2 Bahman Mirzaei,3 Zahra Norouzi Bazgir,1,2 Hamid Reza Goli1,2 1Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; 2Department of Medical Microbiology and Virology, Faculty of Medicine,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-08-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/distribution-and-molecular-characterization-of-resistance-gene-cassett-peer-reviewed-article-IDR |
| _version_ | 1818351513262620672 |
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| author | Ahmadian L Haghshenas MR Mirzaei B Norouzi Bazgir Z Goli HR |
| author_facet | Ahmadian L Haghshenas MR Mirzaei B Norouzi Bazgir Z Goli HR |
| author_sort | Ahmadian L |
| collection | DOAJ |
| description | Leila Ahmadian,1,2 Mohammad Reza Haghshenas,1,2 Bahman Mirzaei,3 Zahra Norouzi Bazgir,1,2 Hamid Reza Goli1,2 1Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; 2Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; 3Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, IranCorrespondence: Hamid Reza GoliDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Farah Abad Blv, Khazar Square, Sari, Mazandaran, IranTel +98 1133543081Fax +98 1133543249Email goli59@gmail.comPurpose: The integrons, as the mobile exogenous elements, play a prominent role in the spreading of antimicrobial resistance genes from Pseudomonas aeruginosa clinical isolates to other bacteria. This study aimed to investigate the frequency of class 1 integrons andresistance gene cassettes carrying by them in clinical isolates as well as multidrug resistant P. aeruginosa.Materials and Methods: A total of 100 clinical isolates of P. aeruginosa were collected from 5 hospitals in Mazandaran province, north Iran. The antibiotic susceptibility pattern of the isolates was evaluated using the disk agar diffusion method. Genomic DNAs were extracted and then the presence of class 1 integrons was detected by the PCR test. All PCR products of the positive isolates were sequenced for the detection of resistance gene cassettes by the Sanger method.Results: Forty-one percent of the clinical isolates were multi-drug resistant. Also, 42% of the isolates were contained class 1 integron, and 61.9% of the integron positive isolates were detected as MDR. We detected 10 different gene cassettes sizing from 0.6 to 3.5 kb in the present study. The sequencing analysis of the internal variable regions of the class 1 integrons showed that the 0.75 kb gene cassette (aadB) was the most frequent resistance gene (54.76%) among all clinical isolates, as well as the MDR isolates. Other resistance genes detected in this study were included: aadA6-orfD (35.71%), aacA4-blaOXA-10 (21.42%), aadB-aacA4-blaOXA-10 (19.04%), blaOXA-10-aacA4-VIM 1 (11.9%), aacA4-catB10 (7.14%), aacA5-aadA1-cmlA5 (7.14%), blaOXA31-aadA2 (4.76%), and aac(3)-Ic-aacA5-cmlA5 (4.76%). To the best of our knowledge, blaOXA-10-aacA4-VIM1 cassette array is detected for the first time in this study.Conclusion: The treatment of infections caused by P. aeruginosa in this region of Iran is a major problem due to the high prevalence of class 1 integrons. It seems that the high prescription of beta-lactams and aminoglycosides for the treatment of these infections may be replaced by other combination therapy stewardships.Keywords: Pseudomonas aeruginosa, class 1 integrons, MDR, resistance gene cassettes |
| first_indexed | 2024-12-13T18:38:56Z |
| format | Article |
| id | doaj.art-dd992d2eaf7249a4b78379bbde1cc40f |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-13T18:38:56Z |
| publishDate | 2020-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-dd992d2eaf7249a4b78379bbde1cc40f2022-12-21T23:35:17ZengDove Medical PressInfection and Drug Resistance1178-69732020-08-01Volume 132773278156146Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosaAhmadian LHaghshenas MRMirzaei BNorouzi Bazgir ZGoli HRLeila Ahmadian,1,2 Mohammad Reza Haghshenas,1,2 Bahman Mirzaei,3 Zahra Norouzi Bazgir,1,2 Hamid Reza Goli1,2 1Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; 2Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran; 3Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, IranCorrespondence: Hamid Reza GoliDepartment of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Farah Abad Blv, Khazar Square, Sari, Mazandaran, IranTel +98 1133543081Fax +98 1133543249Email goli59@gmail.comPurpose: The integrons, as the mobile exogenous elements, play a prominent role in the spreading of antimicrobial resistance genes from Pseudomonas aeruginosa clinical isolates to other bacteria. This study aimed to investigate the frequency of class 1 integrons andresistance gene cassettes carrying by them in clinical isolates as well as multidrug resistant P. aeruginosa.Materials and Methods: A total of 100 clinical isolates of P. aeruginosa were collected from 5 hospitals in Mazandaran province, north Iran. The antibiotic susceptibility pattern of the isolates was evaluated using the disk agar diffusion method. Genomic DNAs were extracted and then the presence of class 1 integrons was detected by the PCR test. All PCR products of the positive isolates were sequenced for the detection of resistance gene cassettes by the Sanger method.Results: Forty-one percent of the clinical isolates were multi-drug resistant. Also, 42% of the isolates were contained class 1 integron, and 61.9% of the integron positive isolates were detected as MDR. We detected 10 different gene cassettes sizing from 0.6 to 3.5 kb in the present study. The sequencing analysis of the internal variable regions of the class 1 integrons showed that the 0.75 kb gene cassette (aadB) was the most frequent resistance gene (54.76%) among all clinical isolates, as well as the MDR isolates. Other resistance genes detected in this study were included: aadA6-orfD (35.71%), aacA4-blaOXA-10 (21.42%), aadB-aacA4-blaOXA-10 (19.04%), blaOXA-10-aacA4-VIM 1 (11.9%), aacA4-catB10 (7.14%), aacA5-aadA1-cmlA5 (7.14%), blaOXA31-aadA2 (4.76%), and aac(3)-Ic-aacA5-cmlA5 (4.76%). To the best of our knowledge, blaOXA-10-aacA4-VIM1 cassette array is detected for the first time in this study.Conclusion: The treatment of infections caused by P. aeruginosa in this region of Iran is a major problem due to the high prevalence of class 1 integrons. It seems that the high prescription of beta-lactams and aminoglycosides for the treatment of these infections may be replaced by other combination therapy stewardships.Keywords: Pseudomonas aeruginosa, class 1 integrons, MDR, resistance gene cassetteshttps://www.dovepress.com/distribution-and-molecular-characterization-of-resistance-gene-cassett-peer-reviewed-article-IDRpseudomonas aeruginosaclass 1 integronsmdrresistance gene cassettes |
| spellingShingle | Ahmadian L Haghshenas MR Mirzaei B Norouzi Bazgir Z Goli HR Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa Infection and Drug Resistance pseudomonas aeruginosa class 1 integrons mdr resistance gene cassettes |
| title | Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa |
| title_full | Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa |
| title_fullStr | Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa |
| title_full_unstemmed | Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa |
| title_short | Distribution and Molecular Characterization of Resistance Gene Cassettes Containing Class 1 Integrons in Multi-Drug Resistant (MDR) Clinical Isolates of Pseudomonas aeruginosa |
| title_sort | distribution and molecular characterization of resistance gene cassettes containing class 1 integrons in multi drug resistant mdr clinical isolates of pseudomonas aeruginosa |
| topic | pseudomonas aeruginosa class 1 integrons mdr resistance gene cassettes |
| url | https://www.dovepress.com/distribution-and-molecular-characterization-of-resistance-gene-cassett-peer-reviewed-article-IDR |
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