Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells
<i>Polygonum multiflorum</i> Thunb. (PM) and derived products are broadly utilized in Chinese traditional medicine. According to our previous research, PM mostly contains polysaccharides, which display a wide range of biological activities. Two water-soluble polysaccharides (PMPs-1 and P...
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MDPI AG
2024-03-01
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| author | Yan Gou Donglin Gu Jing Fan Jianbo Yang Ke Zan Jingjing Liu Hongyu Jin Ying Wang Feng Wei Shuangcheng Ma |
| author_facet | Yan Gou Donglin Gu Jing Fan Jianbo Yang Ke Zan Jingjing Liu Hongyu Jin Ying Wang Feng Wei Shuangcheng Ma |
| author_sort | Yan Gou |
| collection | DOAJ |
| description | <i>Polygonum multiflorum</i> Thunb. (PM) and derived products are broadly utilized in Chinese traditional medicine. According to our previous research, PM mostly contains polysaccharides, which display a wide range of biological activities. Two water-soluble polysaccharides (PMPs-1 and PMPs-2) were obtained from PM by DEAE-Cellulose and Sephadex G-100 column chromatography. Colorimetry, HPGPC-MALLS-RID, HPLC-PDA, methylation, FT-IR, NMR, and SEM were used to characterize these polysaccharides. PMPs-1 and PMPs-2 had average molecular weights of 255.5 and 55.7 kDa, respectively. PMPs-1 consisted of Man, Glc, Gal, and Ara at 0.9:78.6:1.0:1.6 and was a glucan with → 4)-Glc<i>p</i>-(1 → as a backbone. Meanwhile, PMPs-2, an acidic polysaccharide, comprised Rha, GalA, Glc, Gal, and Ara at 3.2:20.3:2.7:1.0:8.3. PMPs-1 and PMPs-2 significantly improved the proliferation of RAW 264.7 cells and induced NO, TNF-α, and IL-6 release. This study reveals that these two polysaccharides can be explored as novel immunomodulators and provide a basis for further development of PM in food and pharmaceutical industries. |
| first_indexed | 2024-04-24T18:17:25Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-24T18:17:25Z |
| publishDate | 2024-03-01 |
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| series | Foods |
| spelling | doaj.art-dd9e099f54214f87971d248add47eaab2024-03-27T13:41:13ZengMDPI AGFoods2304-81582024-03-0113693210.3390/foods13060932Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 CellsYan Gou0Donglin Gu1Jing Fan2Jianbo Yang3Ke Zan4Jingjing Liu5Hongyu Jin6Ying Wang7Feng Wei8Shuangcheng Ma9NMPA Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, Chengdu 611731, ChinaNMPA Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, Chengdu 611731, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaInstitute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102629, ChinaChinese Pharmacopoeia Committee, Beijing 100061, China<i>Polygonum multiflorum</i> Thunb. (PM) and derived products are broadly utilized in Chinese traditional medicine. According to our previous research, PM mostly contains polysaccharides, which display a wide range of biological activities. Two water-soluble polysaccharides (PMPs-1 and PMPs-2) were obtained from PM by DEAE-Cellulose and Sephadex G-100 column chromatography. Colorimetry, HPGPC-MALLS-RID, HPLC-PDA, methylation, FT-IR, NMR, and SEM were used to characterize these polysaccharides. PMPs-1 and PMPs-2 had average molecular weights of 255.5 and 55.7 kDa, respectively. PMPs-1 consisted of Man, Glc, Gal, and Ara at 0.9:78.6:1.0:1.6 and was a glucan with → 4)-Glc<i>p</i>-(1 → as a backbone. Meanwhile, PMPs-2, an acidic polysaccharide, comprised Rha, GalA, Glc, Gal, and Ara at 3.2:20.3:2.7:1.0:8.3. PMPs-1 and PMPs-2 significantly improved the proliferation of RAW 264.7 cells and induced NO, TNF-α, and IL-6 release. This study reveals that these two polysaccharides can be explored as novel immunomodulators and provide a basis for further development of PM in food and pharmaceutical industries.https://www.mdpi.com/2304-8158/13/6/932<i>Polygonum multiflorum</i> Thunb.purificationpolysaccharidesstructural characterizationimmunostimulatory |
| spellingShingle | Yan Gou Donglin Gu Jing Fan Jianbo Yang Ke Zan Jingjing Liu Hongyu Jin Ying Wang Feng Wei Shuangcheng Ma Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells Foods <i>Polygonum multiflorum</i> Thunb. purification polysaccharides structural characterization immunostimulatory |
| title | Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells |
| title_full | Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells |
| title_fullStr | Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells |
| title_full_unstemmed | Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells |
| title_short | Purification and Structural Characterization of Polysaccharides from <i>Polygonum multiflorum</i> Thunb. and Their Immunostimulatory Activity in RAW264.7 Cells |
| title_sort | purification and structural characterization of polysaccharides from i polygonum multiflorum i thunb and their immunostimulatory activity in raw264 7 cells |
| topic | <i>Polygonum multiflorum</i> Thunb. purification polysaccharides structural characterization immunostimulatory |
| url | https://www.mdpi.com/2304-8158/13/6/932 |
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