Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways
It is widely accepted that eutrophication has played an important role in the formation of harmful cyanobacterial blooms in recent decades, which impacts water quality and ecological environment and causes huge economic losses. Algicidal bacteria have a promising application prospect in controlling...
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MDPI AG
2022-07-01
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| author | Yulei Zhang Jieyi Li Zhangxi Hu Dong Chen Feng Li Xianghu Huang Changling Li |
| author_facet | Yulei Zhang Jieyi Li Zhangxi Hu Dong Chen Feng Li Xianghu Huang Changling Li |
| author_sort | Yulei Zhang |
| collection | DOAJ |
| description | It is widely accepted that eutrophication has played an important role in the formation of harmful cyanobacterial blooms in recent decades, which impacts water quality and ecological environment and causes huge economic losses. Algicidal bacteria have a promising application prospect in controlling cyanobacterial blooms in aquaculture water. Here, the process of the algicidal bacterium <i>Brevibacillus laterosporus</i> strain Bl-zj acting on <i>Microcystis aeruginosa</i> was explored using transcriptome analysis to elucidate the algicidal mechanism. The results of the co-culture of bacterium and alga showed a strong alga-lysing effect of <i>B. laterosporus</i> against <i>M. aeruginosa</i> with an extreme morphology deformation of the algal cells. A total of 2744 differentially expressed genes of <i>B. laterosporus</i> were identified, which were mainly involved in the metabolism of amino acid, carbohydrate, and lipid. In the co-cultured group, the expression of genes mainly enriched in valine, leucine and isoleucine degradation, and fatty acid degradation were significantly increased. However, the expression of the genes related to ribosome were mainly inhibited. Transcriptome analysis showed that <i>B. laterosporus</i> obtained ATP and energy by the degradation of valine, leucine, isoleucine, and fatty acids, and destroyed algal cells by efflux pump transporters, secretion of hydrolytic enzymes, antibiotics, proteases, and other secondary metabolites, resulting in algal death and achieving the algicidal effect. |
| first_indexed | 2024-03-09T12:55:22Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T12:55:22Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Toxins |
| spelling | doaj.art-dd9ebe20c9dd41e3a94eb1e6293a7e7b2023-11-30T22:01:34ZengMDPI AGToxins2072-66512022-07-0114749210.3390/toxins14070492Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic PathwaysYulei Zhang0Jieyi Li1Zhangxi Hu2Dong Chen3Feng Li4Xianghu Huang5Changling Li6Department of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaDepartment of Aquaculture, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, ChinaIt is widely accepted that eutrophication has played an important role in the formation of harmful cyanobacterial blooms in recent decades, which impacts water quality and ecological environment and causes huge economic losses. Algicidal bacteria have a promising application prospect in controlling cyanobacterial blooms in aquaculture water. Here, the process of the algicidal bacterium <i>Brevibacillus laterosporus</i> strain Bl-zj acting on <i>Microcystis aeruginosa</i> was explored using transcriptome analysis to elucidate the algicidal mechanism. The results of the co-culture of bacterium and alga showed a strong alga-lysing effect of <i>B. laterosporus</i> against <i>M. aeruginosa</i> with an extreme morphology deformation of the algal cells. A total of 2744 differentially expressed genes of <i>B. laterosporus</i> were identified, which were mainly involved in the metabolism of amino acid, carbohydrate, and lipid. In the co-cultured group, the expression of genes mainly enriched in valine, leucine and isoleucine degradation, and fatty acid degradation were significantly increased. However, the expression of the genes related to ribosome were mainly inhibited. Transcriptome analysis showed that <i>B. laterosporus</i> obtained ATP and energy by the degradation of valine, leucine, isoleucine, and fatty acids, and destroyed algal cells by efflux pump transporters, secretion of hydrolytic enzymes, antibiotics, proteases, and other secondary metabolites, resulting in algal death and achieving the algicidal effect.https://www.mdpi.com/2072-6651/14/7/492<i>Brevibacillus laterosporus</i><i>Microcystis aeruginosa</i>transcriptomealgicidal effectdegradationtransporter |
| spellingShingle | Yulei Zhang Jieyi Li Zhangxi Hu Dong Chen Feng Li Xianghu Huang Changling Li Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways Toxins <i>Brevibacillus laterosporus</i> <i>Microcystis aeruginosa</i> transcriptome algicidal effect degradation transporter |
| title | Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways |
| title_full | Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways |
| title_fullStr | Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways |
| title_full_unstemmed | Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways |
| title_short | Transcriptome Analysis Reveals the Algicidal Mechanism of <i>Brevibacillus laterosporus</i> against <i>Microcystis aeruginosa</i> through Multiple Metabolic Pathways |
| title_sort | transcriptome analysis reveals the algicidal mechanism of i brevibacillus laterosporus i against i microcystis aeruginosa i through multiple metabolic pathways |
| topic | <i>Brevibacillus laterosporus</i> <i>Microcystis aeruginosa</i> transcriptome algicidal effect degradation transporter |
| url | https://www.mdpi.com/2072-6651/14/7/492 |
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