Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases
In Arabidopsis, root hair and non-hair cell fates are determined by a MYB-bHLH-WD40 transcriptional complex and are regulated by many internal and environmental cues. Brassinosteroids play important roles in regulating root hair specification by unknown mechanisms. Here, we systematically examined r...
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| Format: | Article |
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eLife Sciences Publications Ltd
2014-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/02525 |
| _version_ | 1811253333578481664 |
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| author | Yinwei Cheng Wenjiao Zhu Yuxiao Chen Shinsaku Ito Tadao Asami Xuelu Wang |
| author_facet | Yinwei Cheng Wenjiao Zhu Yuxiao Chen Shinsaku Ito Tadao Asami Xuelu Wang |
| author_sort | Yinwei Cheng |
| collection | DOAJ |
| description | In Arabidopsis, root hair and non-hair cell fates are determined by a MYB-bHLH-WD40 transcriptional complex and are regulated by many internal and environmental cues. Brassinosteroids play important roles in regulating root hair specification by unknown mechanisms. Here, we systematically examined root hair phenotypes in brassinosteroid-related mutants, and found that brassinosteroid signaling inhibits root hair formation through GSK3-like kinases or upstream components. We found that with enhanced brassinosteroid signaling, GL2, a cell fate marker for non-hair cells, is ectopically expressed in hair cells, while its expression in non-hair cells is suppressed when brassinosteroid signaling is reduced. Genetic analysis demonstrated that brassinosteroid-regulated root epidermal cell patterning is dependent on the WER-GL3/EGL3-TTG1 transcriptional complex. One of the GSK3-like kinases, BIN2, interacted with and phosphorylated EGL3, and EGL3s mutated at phosphorylation sites were retained in hair cell nuclei. BIN2 phosphorylated TTG1 to inhibit the activity of the WER-GL3/EGL3-TTG1 complex. Thus, our study provides insights into the mechanism of brassinosteroid regulation of root hair patterning. |
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| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:49:18Z |
| publishDate | 2014-04-01 |
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| spelling | doaj.art-dd9f37d3fe914c70b16510eca02a58992022-12-22T03:24:27ZengeLife Sciences Publications LtdeLife2050-084X2014-04-01310.7554/eLife.02525Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinasesYinwei Cheng0Wenjiao Zhu1Yuxiao Chen2Shinsaku Ito3Tadao Asami4Xuelu Wang5State Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, ChinaGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanState Key Laboratory of Genetic Engineering, and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, ChinaIn Arabidopsis, root hair and non-hair cell fates are determined by a MYB-bHLH-WD40 transcriptional complex and are regulated by many internal and environmental cues. Brassinosteroids play important roles in regulating root hair specification by unknown mechanisms. Here, we systematically examined root hair phenotypes in brassinosteroid-related mutants, and found that brassinosteroid signaling inhibits root hair formation through GSK3-like kinases or upstream components. We found that with enhanced brassinosteroid signaling, GL2, a cell fate marker for non-hair cells, is ectopically expressed in hair cells, while its expression in non-hair cells is suppressed when brassinosteroid signaling is reduced. Genetic analysis demonstrated that brassinosteroid-regulated root epidermal cell patterning is dependent on the WER-GL3/EGL3-TTG1 transcriptional complex. One of the GSK3-like kinases, BIN2, interacted with and phosphorylated EGL3, and EGL3s mutated at phosphorylation sites were retained in hair cell nuclei. BIN2 phosphorylated TTG1 to inhibit the activity of the WER-GL3/EGL3-TTG1 complex. Thus, our study provides insights into the mechanism of brassinosteroid regulation of root hair patterning.https://elifesciences.org/articles/02525brassinosteroidGSK3-like kinaseroot epidermal cell fateEGL3phosphorylationTTG1 |
| spellingShingle | Yinwei Cheng Wenjiao Zhu Yuxiao Chen Shinsaku Ito Tadao Asami Xuelu Wang Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases eLife brassinosteroid GSK3-like kinase root epidermal cell fate EGL3 phosphorylation TTG1 |
| title | Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases |
| title_full | Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases |
| title_fullStr | Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases |
| title_full_unstemmed | Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases |
| title_short | Brassinosteroids control root epidermal cell fate via direct regulation of a MYB-bHLH-WD40 complex by GSK3-like kinases |
| title_sort | brassinosteroids control root epidermal cell fate via direct regulation of a myb bhlh wd40 complex by gsk3 like kinases |
| topic | brassinosteroid GSK3-like kinase root epidermal cell fate EGL3 phosphorylation TTG1 |
| url | https://elifesciences.org/articles/02525 |
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