Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed

Effect of ultrasonic power on the structure and functional properties of water-soluble protein extracted from defatted Moringa oleifera seed were explored. The results showed that ultrasonic treatment could reduce β-sheet and β-turn content of water-soluble protein from Moringa oleifera seed (MOWP)...

Full description

Bibliographic Details
Main Authors: Shi-Qi Tang, Qiu-Han Du, Zhen Fu
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Ultrasonics Sonochemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1350417720316618
_version_ 1818441709412941824
author Shi-Qi Tang
Qiu-Han Du
Zhen Fu
author_facet Shi-Qi Tang
Qiu-Han Du
Zhen Fu
author_sort Shi-Qi Tang
collection DOAJ
description Effect of ultrasonic power on the structure and functional properties of water-soluble protein extracted from defatted Moringa oleifera seed were explored. The results showed that ultrasonic treatment could reduce β-sheet and β-turn content of water-soluble protein from Moringa oleifera seed (MOWP) and increase the content of random coil and α-helix. Changes in intrinsic fluorescence spectra, surface hydrophobicity (H0) and thermal behaviors indicated that ultrasonic had significant effect on the tertiary structure of MOWP. The results of SEM and SDS-PAGE showed that the MOWP was aggregated but not significantly degraded by ultrasound. The solubility, foaming properties and emulsifying properties of MOWP increased firstly and then decreased with the increase of ultrasonic power. Ultrasonic treatment altered the functional properties of MOWP, which might be attributed to the exposure of hydrophilic group and the change of and secondary and tertiary structure.
first_indexed 2024-12-14T18:32:34Z
format Article
id doaj.art-dda25503d09442c881516cb48b19ff20
institution Directory Open Access Journal
issn 1350-4177
language English
last_indexed 2024-12-14T18:32:34Z
publishDate 2021-03-01
publisher Elsevier
record_format Article
series Ultrasonics Sonochemistry
spelling doaj.art-dda25503d09442c881516cb48b19ff202022-12-21T22:51:43ZengElsevierUltrasonics Sonochemistry1350-41772021-03-0171105357Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seedShi-Qi Tang0Qiu-Han Du1Zhen Fu2Institute of Light Industry and Food Engineering, Guangxi University, 53004 Nanning, ChinaInstitute of Light Industry and Food Engineering, Guangxi University, 53004 Nanning, ChinaCorresponding author.; Institute of Light Industry and Food Engineering, Guangxi University, 53004 Nanning, ChinaEffect of ultrasonic power on the structure and functional properties of water-soluble protein extracted from defatted Moringa oleifera seed were explored. The results showed that ultrasonic treatment could reduce β-sheet and β-turn content of water-soluble protein from Moringa oleifera seed (MOWP) and increase the content of random coil and α-helix. Changes in intrinsic fluorescence spectra, surface hydrophobicity (H0) and thermal behaviors indicated that ultrasonic had significant effect on the tertiary structure of MOWP. The results of SEM and SDS-PAGE showed that the MOWP was aggregated but not significantly degraded by ultrasound. The solubility, foaming properties and emulsifying properties of MOWP increased firstly and then decreased with the increase of ultrasonic power. Ultrasonic treatment altered the functional properties of MOWP, which might be attributed to the exposure of hydrophilic group and the change of and secondary and tertiary structure.http://www.sciencedirect.com/science/article/pii/S1350417720316618Moringa oleifera seedProteinStructurePhysicochemical properties
spellingShingle Shi-Qi Tang
Qiu-Han Du
Zhen Fu
Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed
Ultrasonics Sonochemistry
Moringa oleifera seed
Protein
Structure
Physicochemical properties
title Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed
title_full Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed
title_fullStr Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed
title_full_unstemmed Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed
title_short Ultrasonic treatment on physicochemical properties of water-soluble protein from Moringa oleifera seed
title_sort ultrasonic treatment on physicochemical properties of water soluble protein from moringa oleifera seed
topic Moringa oleifera seed
Protein
Structure
Physicochemical properties
url http://www.sciencedirect.com/science/article/pii/S1350417720316618
work_keys_str_mv AT shiqitang ultrasonictreatmentonphysicochemicalpropertiesofwatersolubleproteinfrommoringaoleiferaseed
AT qiuhandu ultrasonictreatmentonphysicochemicalpropertiesofwatersolubleproteinfrommoringaoleiferaseed
AT zhenfu ultrasonictreatmentonphysicochemicalpropertiesofwatersolubleproteinfrommoringaoleiferaseed