Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico
Propolis collected by stingless bees is a valuable biocultural resource and a source of bioactive compounds. Methodologies to establish both the geographic origin and the potential pharmacological activity of propolis of stingless bees are required to regulate their sustainable use. The aim of this...
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MDPI AG
2023-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/7/1434 |
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| author | Jorge Carlos Ruiz Ruiz Neith Aracely Pacheco López Estephania Guadalupe Rejón Méndez Felipe Antonio Samos López Luis Medina Medina José Javier G. Quezada-Euán |
| author_facet | Jorge Carlos Ruiz Ruiz Neith Aracely Pacheco López Estephania Guadalupe Rejón Méndez Felipe Antonio Samos López Luis Medina Medina José Javier G. Quezada-Euán |
| author_sort | Jorge Carlos Ruiz Ruiz |
| collection | DOAJ |
| description | Propolis collected by stingless bees is a valuable biocultural resource and a source of bioactive compounds. Methodologies to establish both the geographic origin and the potential pharmacological activity of propolis of stingless bees are required to regulate their sustainable use. The aim of this study was to classify <i>Melipona beecheii</i> propolis according to its phenolic compound content and potential pharmacological activity, using in vitro assays and statistical methodologies of multivariate analysis, hierarchical cluster analysis, and principal component analysis. Propolis samples were collected from seven states in southeastern Mexico. Total phenolic content and flavonoids were determined spectrophotometrically, and antioxidant, anti-inflammatory, and antimicrobial activities were evaluated. Both total phenolic content and flavonoids, and in vitro bioactivity potential of propolis extracts showed significant variations. Multivariate analysis, hierarchical cluster analysis, and principal component analysis enabled us to distinguish and classify propolis produced by <i>M. beecheii</i> according to similarity in terms of total phenolic content, in vitro bioactivity potential, and geographical origin. This strategy could be used to establish regulations for sustainable use, marketing, and industrial applications. |
| first_indexed | 2024-03-11T05:38:13Z |
| format | Article |
| id | doaj.art-dda29c2daa924c7ea226cfeab834b0e6 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:38:13Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-dda29c2daa924c7ea226cfeab834b0e62023-11-17T16:41:16ZengMDPI AGFoods2304-81582023-03-01127143410.3390/foods12071434Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern MexicoJorge Carlos Ruiz Ruiz0Neith Aracely Pacheco López1Estephania Guadalupe Rejón Méndez2Felipe Antonio Samos López3Luis Medina Medina4José Javier G. Quezada-Euán5Escuela de Nutrición, División de Ciencias de la Salud, Universidad Anáhuac-Mayab, Km 15.5 Carretera Mérida a Progreso, Int. Km 2 Carretera a Chablekal, Mérida 97310, Yucatán, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Subsede Sureste, Parque Científico Tecnológico de Yucatán, Km 5.5 Sierra Papacal-Chuburná Puerto, Mérida 97302, Yucatán, MexicoDepartamento de Apicultura, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Apartado Postal 4-116, Mérida 97100, Yucatán, MexicoDepartamento de Apicultura, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Apartado Postal 4-116, Mérida 97100, Yucatán, MexicoDepartamento de Apicultura, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Apartado Postal 4-116, Mérida 97100, Yucatán, MexicoDepartamento de Apicultura, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Apartado Postal 4-116, Mérida 97100, Yucatán, MexicoPropolis collected by stingless bees is a valuable biocultural resource and a source of bioactive compounds. Methodologies to establish both the geographic origin and the potential pharmacological activity of propolis of stingless bees are required to regulate their sustainable use. The aim of this study was to classify <i>Melipona beecheii</i> propolis according to its phenolic compound content and potential pharmacological activity, using in vitro assays and statistical methodologies of multivariate analysis, hierarchical cluster analysis, and principal component analysis. Propolis samples were collected from seven states in southeastern Mexico. Total phenolic content and flavonoids were determined spectrophotometrically, and antioxidant, anti-inflammatory, and antimicrobial activities were evaluated. Both total phenolic content and flavonoids, and in vitro bioactivity potential of propolis extracts showed significant variations. Multivariate analysis, hierarchical cluster analysis, and principal component analysis enabled us to distinguish and classify propolis produced by <i>M. beecheii</i> according to similarity in terms of total phenolic content, in vitro bioactivity potential, and geographical origin. This strategy could be used to establish regulations for sustainable use, marketing, and industrial applications.https://www.mdpi.com/2304-8158/12/7/1434geographicalclassificationstingless beespropolisphytochemicalsin vitro bioactivity potential |
| spellingShingle | Jorge Carlos Ruiz Ruiz Neith Aracely Pacheco López Estephania Guadalupe Rejón Méndez Felipe Antonio Samos López Luis Medina Medina José Javier G. Quezada-Euán Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico Foods geographical classification stingless bees propolis phytochemicals in vitro bioactivity potential |
| title | Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico |
| title_full | Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico |
| title_fullStr | Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico |
| title_full_unstemmed | Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico |
| title_short | Phenolic Content and Bioactivity as Geographical Classifiers of Propolis from Stingless Bees in Southeastern Mexico |
| title_sort | phenolic content and bioactivity as geographical classifiers of propolis from stingless bees in southeastern mexico |
| topic | geographical classification stingless bees propolis phytochemicals in vitro bioactivity potential |
| url | https://www.mdpi.com/2304-8158/12/7/1434 |
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