Summary: | Recently, the biosynthesis of zinc oxide nanoparticles (ZnO NPs) from crude extracts and phytochemicals has attracted much attention. Green synthesis of NPs is cost-effective, eco-friendly, and is a promising alternative for chemical synthesis. This study involves ZnO NPs synthesis using <i>Rubus fairholmianus</i> root extract (RE) as an efficient reducing agent. The UV spectrum of RE-ZnO NPs exhibited a peak at 357 nm due to intrinsic bandgap absorption and an XRD pattern that matches the ZnO crystal structure (JCPDS card no: 36-1451). The average particle size calculated from the Debye–Scherrer equation is 11.34 nm. SEM analysis showed that the RE-ZnO NPs spherical in shape with clusters (1–100 nm). The antibacterial activity of the NPs was tested against <i>Staphylococcus aureus</i> using agar well diffusion, minimum inhibitory concentration, and bacterial growth assay. The <i>R. fairholmianus</i> phytochemicals facilitate the synthesis of stable ZnO NPs and showed antibacterial activity.
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