Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad
Phytases are enzyme capable of hydrolysing phytic acid to myo-inositol and inorganic phosphorus. The aim of the present study was the screening and isolation of efficient phytase producing bacteria from soil samples. 14 bacterial isolates were found to produce clear zone of hydrolysis on PSM contain...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2017-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/isolation-identification-and-molecular-characterization-of-phytase-producing-bacteria-pseudomonas-sp-aazad/ |
| _version_ | 1818977290906763264 |
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| author | Ch. Shalu Singh Sarita Singh P.K. Sharma Deepak Chand Sharma |
| author_facet | Ch. Shalu Singh Sarita Singh P.K. Sharma Deepak Chand Sharma |
| author_sort | Ch. Shalu Singh |
| collection | DOAJ |
| description | Phytases are enzyme capable of hydrolysing phytic acid to myo-inositol and inorganic phosphorus. The aim of the present study was the screening and isolation of efficient phytase producing bacteria from soil samples. 14 bacterial isolates were found to produce clear zone of hydrolysis on PSM containing 0.5% ca-phytate. Out of all phytase positive isolates, five bacterial isolates 100A, 9H, 14A, 4D, and 2B showed significant phytase production in submerged fermentation. The isolate 14A was found to be the best among all with the maximum production of 89.09 U/ml after 72 h of incubation at
30 ºC. Colony morphology and microscopic examination (Gram’s staining) of maximal phytase producer suggested that the isolate 14A is gram negative rod. Further on molecular analysis the bacterial isolate showed closest similarity with Pseudomonas sp. 16S rDNA sequencing and sequence similarity search using BLAST showed that it matches 97 % with Pseudomonas pulluriniana, while 3% was found to be unique sequence and named as Pseudomonas sp. aazad. The crude phytase from the isolate showed optimum activity at pH 6 and temperature 50 ºC. |
| first_indexed | 2024-12-20T16:25:24Z |
| format | Article |
| id | doaj.art-dda57755ad4549babf5eb56221d7f593 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-20T16:25:24Z |
| publishDate | 2017-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-dda57755ad4549babf5eb56221d7f5932022-12-21T19:33:28ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-12-011141845185010.22207/JPAM.11.4.24Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazadCh. Shalu Singh0Sarita Singh1P.K. Sharma2Deepak Chand Sharma3Department of Microbiology, Ch. Charan Singh University, Meerut - 250 004, India. Department of Microbiology, Ch. Charan Singh University, Meerut - 250 004, India. Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut - 250 004, India. Department of Microbiology, Dr. Shakuntala Misra National Rehabilitation University, Lucknow. India. Phytases are enzyme capable of hydrolysing phytic acid to myo-inositol and inorganic phosphorus. The aim of the present study was the screening and isolation of efficient phytase producing bacteria from soil samples. 14 bacterial isolates were found to produce clear zone of hydrolysis on PSM containing 0.5% ca-phytate. Out of all phytase positive isolates, five bacterial isolates 100A, 9H, 14A, 4D, and 2B showed significant phytase production in submerged fermentation. The isolate 14A was found to be the best among all with the maximum production of 89.09 U/ml after 72 h of incubation at 30 ºC. Colony morphology and microscopic examination (Gram’s staining) of maximal phytase producer suggested that the isolate 14A is gram negative rod. Further on molecular analysis the bacterial isolate showed closest similarity with Pseudomonas sp. 16S rDNA sequencing and sequence similarity search using BLAST showed that it matches 97 % with Pseudomonas pulluriniana, while 3% was found to be unique sequence and named as Pseudomonas sp. aazad. The crude phytase from the isolate showed optimum activity at pH 6 and temperature 50 ºC.https://microbiologyjournal.org/isolation-identification-and-molecular-characterization-of-phytase-producing-bacteria-pseudomonas-sp-aazad/phytasepsmpseudomonas sp.ca-phytatefermentation |
| spellingShingle | Ch. Shalu Singh Sarita Singh P.K. Sharma Deepak Chand Sharma Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad Journal of Pure and Applied Microbiology phytase psm pseudomonas sp. ca-phytate fermentation |
| title | Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad |
| title_full | Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad |
| title_fullStr | Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad |
| title_full_unstemmed | Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad |
| title_short | Isolation, Identification and Molecular Characterization of Phytase Producing Bacteria, Pseudomonas Sp. aazad |
| title_sort | isolation identification and molecular characterization of phytase producing bacteria pseudomonas sp aazad |
| topic | phytase psm pseudomonas sp. ca-phytate fermentation |
| url | https://microbiologyjournal.org/isolation-identification-and-molecular-characterization-of-phytase-producing-bacteria-pseudomonas-sp-aazad/ |
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