Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle
The objective of the present study was to investigate the potential role of immunization against INH on follicular development, serum reproductive hormone (FSH, E2, and P4) concentrations, and reproductive performance in beef cattle. A total of 196 non-lactating female beef cattle (4-5 years old) wi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1275022/full |
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| author | Jinzhu Meng Jinzhu Meng Qiuye Li Lilin Xiao Weichen Liu Zhengjie Gao Lin Gong Xianyong Lan Shuilian Wang |
| author_facet | Jinzhu Meng Jinzhu Meng Qiuye Li Lilin Xiao Weichen Liu Zhengjie Gao Lin Gong Xianyong Lan Shuilian Wang |
| author_sort | Jinzhu Meng |
| collection | DOAJ |
| description | The objective of the present study was to investigate the potential role of immunization against INH on follicular development, serum reproductive hormone (FSH, E2, and P4) concentrations, and reproductive performance in beef cattle. A total of 196 non-lactating female beef cattle (4-5 years old) with identical calving records (3 records) were immunized with 0.5, 1.0, 1.5, or 2.0 mg [(T1, n = 58), (T2, n = 46), (T3, n = 42) and (T4, n = 36), respectively] of the pcISI plasmid. The control (C) group (n = 14) was immunized with 1.0 mL 0.9% saline. At 21d after primary immunization, all beef cattle were boosted with half of the primary immunization dose. On day 10 after primary immunization, the beef cattle immunized with INH DNA vaccine evidently induced anti-INH antibody except for the T1 group. The T3 group had the greatest P/N value peak among all the groups. The anti-INH antibody positive rates in T2, T3 and T4 groups were significantly higher than that in C and T1 groups. RIA results indicated that serum FSH concentration in T2 group increased markedly on day 45 after booster immunization; the E2 amount in T3 group was significantly increased on day 10 after primary immunization, and the levels of E2 also improved in T2 and T3 groups after booster immunization; the P4 concentration in T2 group was significantly improved on day 21 after primary immunization. Ultrasonography results revealed that the follicles with different diameter sizes were increased, meanwhile, the diameter and growth speed of ovulatory follicle were significantly increased. Furthermore, the rates of estrous, ovulation, conception, and twinning rate were also significantly enhanced. These findings clearly illustrated that INH DNA vaccine was capable of promoting the follicle development, thereby improving the behavioral of estrous and ovulation, eventually leading to an augment in the conception rates and twinning rate of beef cattle. |
| first_indexed | 2024-03-07T23:15:29Z |
| format | Article |
| id | doaj.art-dda7474d672b4437a74b3d2655aa9ed3 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-07T23:15:29Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-dda7474d672b4437a74b3d2655aa9ed32024-02-21T13:03:38ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-02-011410.3389/fendo.2023.12750221275022Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattleJinzhu Meng0Jinzhu Meng1Qiuye Li2Lilin Xiao3Weichen Liu4Zhengjie Gao5Lin Gong6Xianyong Lan7Shuilian Wang8College of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaGuizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaThe objective of the present study was to investigate the potential role of immunization against INH on follicular development, serum reproductive hormone (FSH, E2, and P4) concentrations, and reproductive performance in beef cattle. A total of 196 non-lactating female beef cattle (4-5 years old) with identical calving records (3 records) were immunized with 0.5, 1.0, 1.5, or 2.0 mg [(T1, n = 58), (T2, n = 46), (T3, n = 42) and (T4, n = 36), respectively] of the pcISI plasmid. The control (C) group (n = 14) was immunized with 1.0 mL 0.9% saline. At 21d after primary immunization, all beef cattle were boosted with half of the primary immunization dose. On day 10 after primary immunization, the beef cattle immunized with INH DNA vaccine evidently induced anti-INH antibody except for the T1 group. The T3 group had the greatest P/N value peak among all the groups. The anti-INH antibody positive rates in T2, T3 and T4 groups were significantly higher than that in C and T1 groups. RIA results indicated that serum FSH concentration in T2 group increased markedly on day 45 after booster immunization; the E2 amount in T3 group was significantly increased on day 10 after primary immunization, and the levels of E2 also improved in T2 and T3 groups after booster immunization; the P4 concentration in T2 group was significantly improved on day 21 after primary immunization. Ultrasonography results revealed that the follicles with different diameter sizes were increased, meanwhile, the diameter and growth speed of ovulatory follicle were significantly increased. Furthermore, the rates of estrous, ovulation, conception, and twinning rate were also significantly enhanced. These findings clearly illustrated that INH DNA vaccine was capable of promoting the follicle development, thereby improving the behavioral of estrous and ovulation, eventually leading to an augment in the conception rates and twinning rate of beef cattle.https://www.frontiersin.org/articles/10.3389/fendo.2023.1275022/fullinhibinvaccineimmunizationreproductive performancebeef cattle |
| spellingShingle | Jinzhu Meng Jinzhu Meng Qiuye Li Lilin Xiao Weichen Liu Zhengjie Gao Lin Gong Xianyong Lan Shuilian Wang Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle Frontiers in Endocrinology inhibin vaccine immunization reproductive performance beef cattle |
| title | Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle |
| title_full | Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle |
| title_fullStr | Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle |
| title_full_unstemmed | Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle |
| title_short | Immunization against inhibin DNA vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle |
| title_sort | immunization against inhibin dna vaccine as an alternative therapeutic for improving follicle development and reproductive performance in beef cattle |
| topic | inhibin vaccine immunization reproductive performance beef cattle |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1275022/full |
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