Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i>
The spread of multidrug-resistant (MDR) <i>Vibrio cholerae</i> necessitates the development of novel prevention and treatment strategies. This study aims to evaluate the in vitro antibacterial activity of green tea polyphenol (−)-epigallocatechin-3-gallate (EGCG) against MDR <i>V....
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| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/4/518 |
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| author | Achiraya Siriphap Anong Kiddee Acharaporn Duangjai Atchariya Yosboonruang Grissana Pook-In Surasak Saokaew Orasa Sutheinkul Anchalee Rawangkan |
| author_facet | Achiraya Siriphap Anong Kiddee Acharaporn Duangjai Atchariya Yosboonruang Grissana Pook-In Surasak Saokaew Orasa Sutheinkul Anchalee Rawangkan |
| author_sort | Achiraya Siriphap |
| collection | DOAJ |
| description | The spread of multidrug-resistant (MDR) <i>Vibrio cholerae</i> necessitates the development of novel prevention and treatment strategies. This study aims to evaluate the in vitro antibacterial activity of green tea polyphenol (−)-epigallocatechin-3-gallate (EGCG) against MDR <i>V. cholerae</i>. First, MIC and MBC values were evaluated by broth microdilution techniques against 45 <i>V. cholerae</i> strains. The checkerboard assay was then used to determine the synergistic effect of EGCG and tetracycline. The pharmaceutical mode of action of EGCG was clarified by time-killing kinetics and membrane disruption assay. Our results revealed that all of the 45 clinical isolates were susceptible to EGCG, with MIC and MBC values in the range of 62.5–250 µg/mL and 125–500 µg/mL, respectively. Furthermore, the combination of EGCG and tetracycline was greater than either treatment alone, with a fractional inhibitory concentration index (FICI) of 0.009 and 0.018 in the O1 and O139 representative serotypes, respectively. Time-killing kinetics analysis suggested that EGCG had bactericidal activity for MDR <i>V. cholerae</i> after exposure to at least 62.5 µg/mL EGCG within 1 h. The mode of action of EGCG might be associated with membrane disrupting permeability, as confirmed by scanning electron microscopy. This is the first indication that EGCG is a viable anti-MDR <i>V. cholerae</i> treatment. |
| first_indexed | 2024-03-09T11:15:24Z |
| format | Article |
| id | doaj.art-dda795f77fd24f12b35eda5c8e6cdd7f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:15:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-dda795f77fd24f12b35eda5c8e6cdd7f2023-12-01T00:33:16ZengMDPI AGAntibiotics2079-63822022-04-0111451810.3390/antibiotics11040518Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i>Achiraya Siriphap0Anong Kiddee1Acharaporn Duangjai2Atchariya Yosboonruang3Grissana Pook-In4Surasak Saokaew5Orasa Sutheinkul6Anchalee Rawangkan7School of Medical Sciences, University of Phayao, Phayao 56000, ThailandSchool of Medical Sciences, University of Phayao, Phayao 56000, ThailandSchool of Medical Sciences, University of Phayao, Phayao 56000, ThailandSchool of Medical Sciences, University of Phayao, Phayao 56000, ThailandSchool of Medical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Care, Division of Social and Administrative Pharmacy, School of Pharmaceutical Sciences, University of Phayao, Phayao 56000, ThailandFaculty of Public Health, Mahidol University, Bangkok 10400, ThailandSchool of Medical Sciences, University of Phayao, Phayao 56000, ThailandThe spread of multidrug-resistant (MDR) <i>Vibrio cholerae</i> necessitates the development of novel prevention and treatment strategies. This study aims to evaluate the in vitro antibacterial activity of green tea polyphenol (−)-epigallocatechin-3-gallate (EGCG) against MDR <i>V. cholerae</i>. First, MIC and MBC values were evaluated by broth microdilution techniques against 45 <i>V. cholerae</i> strains. The checkerboard assay was then used to determine the synergistic effect of EGCG and tetracycline. The pharmaceutical mode of action of EGCG was clarified by time-killing kinetics and membrane disruption assay. Our results revealed that all of the 45 clinical isolates were susceptible to EGCG, with MIC and MBC values in the range of 62.5–250 µg/mL and 125–500 µg/mL, respectively. Furthermore, the combination of EGCG and tetracycline was greater than either treatment alone, with a fractional inhibitory concentration index (FICI) of 0.009 and 0.018 in the O1 and O139 representative serotypes, respectively. Time-killing kinetics analysis suggested that EGCG had bactericidal activity for MDR <i>V. cholerae</i> after exposure to at least 62.5 µg/mL EGCG within 1 h. The mode of action of EGCG might be associated with membrane disrupting permeability, as confirmed by scanning electron microscopy. This is the first indication that EGCG is a viable anti-MDR <i>V. cholerae</i> treatment.https://www.mdpi.com/2079-6382/11/4/518antimicrobial activityEGCGgreen teaMDR<i>Vibrio cholerae</i> |
| spellingShingle | Achiraya Siriphap Anong Kiddee Acharaporn Duangjai Atchariya Yosboonruang Grissana Pook-In Surasak Saokaew Orasa Sutheinkul Anchalee Rawangkan Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i> Antibiotics antimicrobial activity EGCG green tea MDR <i>Vibrio cholerae</i> |
| title | Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i> |
| title_full | Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i> |
| title_fullStr | Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i> |
| title_full_unstemmed | Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i> |
| title_short | Antimicrobial Activity of the Green Tea Polyphenol (−)-Epigallocatechin-3-Gallate (EGCG) against Clinical Isolates of Multidrug-Resistant <i>Vibrio cholerae</i> |
| title_sort | antimicrobial activity of the green tea polyphenol epigallocatechin 3 gallate egcg against clinical isolates of multidrug resistant i vibrio cholerae i |
| topic | antimicrobial activity EGCG green tea MDR <i>Vibrio cholerae</i> |
| url | https://www.mdpi.com/2079-6382/11/4/518 |
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