A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing
The healing process of a diabetic wound (DW) is often impeded by a series of interrelated factors, including severe infection, persistent inflammation, and excessive oxidative stress. Therefore, it is particularly crucial to develop a medical dressing that can address these issues simultaneously. To...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16286 |
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| author | Zhengbo Hu Kai Zhao Xingcan Chen Mingyuan Zhou Yuchi Chen Xiaoqing Ye Fangmei Zhou Zhishan Ding Bingqi Zhu |
| author_facet | Zhengbo Hu Kai Zhao Xingcan Chen Mingyuan Zhou Yuchi Chen Xiaoqing Ye Fangmei Zhou Zhishan Ding Bingqi Zhu |
| author_sort | Zhengbo Hu |
| collection | DOAJ |
| description | The healing process of a diabetic wound (DW) is often impeded by a series of interrelated factors, including severe infection, persistent inflammation, and excessive oxidative stress. Therefore, it is particularly crucial to develop a medical dressing that can address these issues simultaneously. To this end, different ratios of <i>Bletilla striata</i> polysaccharide (BSP) and berberine (BER) were physically blended with Carbomer 940 (CBM940) to develop a composite hydrogel as a medical dressing. The BSP/BER hydrogel was characterized using SEM, FTIR, rheological testing and other techniques. The anti-inflammatory, antioxidant, and antibacterial properties of the hydrogel were evaluated using cell and bacterial models in vitro. A DW model of ICR mice was established to evaluate the effect of the hydrogel on DW healing in vivo. The hydrogel exhibited excellent biocompatibility and remarkable antibacterial, anti-inflammatory, and antioxidant properties. In addition, animal experiments showed that the BSP/BER hydrogel significantly accelerated wound healing in DW mice. Among the different formulations, the LBSP/BER hydrogel (2% BSP, m<sub>BER</sub>:m<sub>BSP</sub> = 1:40) demonstrated the most remarkable efficacy. In conclusion, the BSP/BER hydrogel developed exhibited immense properties and great potential as a medical dressing for the repair of DW, addressing a crucial need in clinical practice. |
| first_indexed | 2024-03-09T16:46:22Z |
| format | Article |
| id | doaj.art-dda7cf8f1ea542c48dc358ef427df561 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:46:22Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dda7cf8f1ea542c48dc358ef427df5612023-11-24T14:47:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221628610.3390/ijms242216286A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound HealingZhengbo Hu0Kai Zhao1Xingcan Chen2Mingyuan Zhou3Yuchi Chen4Xiaoqing Ye5Fangmei Zhou6Zhishan Ding7Bingqi Zhu8School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaThe healing process of a diabetic wound (DW) is often impeded by a series of interrelated factors, including severe infection, persistent inflammation, and excessive oxidative stress. Therefore, it is particularly crucial to develop a medical dressing that can address these issues simultaneously. To this end, different ratios of <i>Bletilla striata</i> polysaccharide (BSP) and berberine (BER) were physically blended with Carbomer 940 (CBM940) to develop a composite hydrogel as a medical dressing. The BSP/BER hydrogel was characterized using SEM, FTIR, rheological testing and other techniques. The anti-inflammatory, antioxidant, and antibacterial properties of the hydrogel were evaluated using cell and bacterial models in vitro. A DW model of ICR mice was established to evaluate the effect of the hydrogel on DW healing in vivo. The hydrogel exhibited excellent biocompatibility and remarkable antibacterial, anti-inflammatory, and antioxidant properties. In addition, animal experiments showed that the BSP/BER hydrogel significantly accelerated wound healing in DW mice. Among the different formulations, the LBSP/BER hydrogel (2% BSP, m<sub>BER</sub>:m<sub>BSP</sub> = 1:40) demonstrated the most remarkable efficacy. In conclusion, the BSP/BER hydrogel developed exhibited immense properties and great potential as a medical dressing for the repair of DW, addressing a crucial need in clinical practice.https://www.mdpi.com/1422-0067/24/22/16286diabetic wound<i>Bletilla striata</i> polysaccharideberberinehydrogel |
| spellingShingle | Zhengbo Hu Kai Zhao Xingcan Chen Mingyuan Zhou Yuchi Chen Xiaoqing Ye Fangmei Zhou Zhishan Ding Bingqi Zhu A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing International Journal of Molecular Sciences diabetic wound <i>Bletilla striata</i> polysaccharide berberine hydrogel |
| title | A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing |
| title_full | A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing |
| title_fullStr | A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing |
| title_full_unstemmed | A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing |
| title_short | A Berberine-Loaded <i>Bletilla striata</i> Polysaccharide Hydrogel as a New Medical Dressing for Diabetic Wound Healing |
| title_sort | berberine loaded i bletilla striata i polysaccharide hydrogel as a new medical dressing for diabetic wound healing |
| topic | diabetic wound <i>Bletilla striata</i> polysaccharide berberine hydrogel |
| url | https://www.mdpi.com/1422-0067/24/22/16286 |
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