Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers
IntroductionPhase angle (PA) is derived from bioelectrical impedance analysis (BIA). It reflects cell membrane function and decreases in disease. It is affected by inflammation, oxidative stress, and diet. Platelet-activating factor (PAF) is a potent inflammatory lipid mediator. Its levels, along wi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1237086/full |
| _version_ | 1827773583657533440 |
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| author | Paraskevi Detopoulou Elizabeth Fragopoulou Tzortzis Nomikos Smaragdi Antonopoulou |
| author_facet | Paraskevi Detopoulou Elizabeth Fragopoulou Tzortzis Nomikos Smaragdi Antonopoulou |
| author_sort | Paraskevi Detopoulou |
| collection | DOAJ |
| description | IntroductionPhase angle (PA) is derived from bioelectrical impedance analysis (BIA). It reflects cell membrane function and decreases in disease. It is affected by inflammation, oxidative stress, and diet. Platelet-activating factor (PAF) is a potent inflammatory lipid mediator. Its levels, along with the activity of its metabolic enzymes, including CDP-choline:1-alkyl-2-acetyl-sn-glycerol-cholinephosphotransferase, acetyl-CoA:lyso-PAF-acetyltransferases, and PAF-AH/Lp-PLA2 are also related to dietary factors, such as the dietary antioxidant capacity (DAC). The aim of the study was to estimate whether the PAF metabolic circuit and related dietary factors are associated with PA in healthy volunteers.MethodsIn healthy subjects, PAF, its metabolic enzyme activity, and erythrocyte fatty acids were measured, while desaturases were estimated. Food-frequency questionnaires and recalls were used, and food groups, macronutrient intake, MedDietScore, and DAC were assessed. Lifestyle and biochemical variables were collected. DXA and BIA measurements were performed.ResultsLp-PLA2 activity was positively associated with PA (rho = 0.651, p < 0.001, total population; rho = 0.780, p < 0.001, women), while PAF levels were negatively associated with PA only in men (partial rho = −0.627, p = 0.012) and inversely related to DAC. Estimated desaturase 6 was inversely associated with PA (rho = −0.404, p = 0.01, total sample). Moreover, the DAC correlated positively with PA (rho = 0.513, p = 0.03, women). All correlations were adjusted for age, body mass index, and sex (if applicable).ConclusionPA is associated with PAF levels and Lp-PLA2 activity in a gender-dependent fashion, indicating the involvement of PAF in cell membrane impairment. The relationship of PA with DAC suggests a protective effect of antioxidants on cellular health, considering that antioxidants may inhibit PAF generation. |
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| format | Article |
| id | doaj.art-dda8aece27ff496cb6f979427151f76b |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-11T13:24:57Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-dda8aece27ff496cb6f979427151f76b2023-11-03T08:59:33ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-11-011010.3389/fnut.2023.12370861237086Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteersParaskevi DetopoulouElizabeth FragopoulouTzortzis NomikosSmaragdi AntonopoulouIntroductionPhase angle (PA) is derived from bioelectrical impedance analysis (BIA). It reflects cell membrane function and decreases in disease. It is affected by inflammation, oxidative stress, and diet. Platelet-activating factor (PAF) is a potent inflammatory lipid mediator. Its levels, along with the activity of its metabolic enzymes, including CDP-choline:1-alkyl-2-acetyl-sn-glycerol-cholinephosphotransferase, acetyl-CoA:lyso-PAF-acetyltransferases, and PAF-AH/Lp-PLA2 are also related to dietary factors, such as the dietary antioxidant capacity (DAC). The aim of the study was to estimate whether the PAF metabolic circuit and related dietary factors are associated with PA in healthy volunteers.MethodsIn healthy subjects, PAF, its metabolic enzyme activity, and erythrocyte fatty acids were measured, while desaturases were estimated. Food-frequency questionnaires and recalls were used, and food groups, macronutrient intake, MedDietScore, and DAC were assessed. Lifestyle and biochemical variables were collected. DXA and BIA measurements were performed.ResultsLp-PLA2 activity was positively associated with PA (rho = 0.651, p < 0.001, total population; rho = 0.780, p < 0.001, women), while PAF levels were negatively associated with PA only in men (partial rho = −0.627, p = 0.012) and inversely related to DAC. Estimated desaturase 6 was inversely associated with PA (rho = −0.404, p = 0.01, total sample). Moreover, the DAC correlated positively with PA (rho = 0.513, p = 0.03, women). All correlations were adjusted for age, body mass index, and sex (if applicable).ConclusionPA is associated with PAF levels and Lp-PLA2 activity in a gender-dependent fashion, indicating the involvement of PAF in cell membrane impairment. The relationship of PA with DAC suggests a protective effect of antioxidants on cellular health, considering that antioxidants may inhibit PAF generation.https://www.frontiersin.org/articles/10.3389/fnut.2023.1237086/fullphase anglebioelectrical impedance analysiserythrocyte fatty acidsplatelet-activating factordietary antioxidant capacityMediterranean diet |
| spellingShingle | Paraskevi Detopoulou Elizabeth Fragopoulou Tzortzis Nomikos Smaragdi Antonopoulou Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers Frontiers in Nutrition phase angle bioelectrical impedance analysis erythrocyte fatty acids platelet-activating factor dietary antioxidant capacity Mediterranean diet |
| title | Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers |
| title_full | Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers |
| title_fullStr | Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers |
| title_full_unstemmed | Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers |
| title_short | Associations of phase angle with platelet-activating factor metabolism and related dietary factors in healthy volunteers |
| title_sort | associations of phase angle with platelet activating factor metabolism and related dietary factors in healthy volunteers |
| topic | phase angle bioelectrical impedance analysis erythrocyte fatty acids platelet-activating factor dietary antioxidant capacity Mediterranean diet |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1237086/full |
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