Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains pr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kerman University of Medical Sciences
2020-06-01
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| Series: | Environmental Health Engineering and Management |
| Subjects: | |
| Online Access: | http://ehemj.com/article-1-615-en.html |
| Summary: | Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to
their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was
investigated in an oil field using indigenous bacterial consortium.
Methods: The bacterial strains present in the contaminated and non-contaminated soils were
identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total
petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were
determined using a gas chromatograph equipped with a flame ionization detector (GC-FID).
Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the
families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from
those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae
(84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial
strains was less in oil-contaminated soils and varied significantly between case and control samples.
Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of
heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced
as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is
one of the innovative aspects of this research.
Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to
bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency
of 28.1% was obtained. |
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| ISSN: | 2423-3765 2423-4311 |