Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains pr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2020-06-01
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| Series: | Environmental Health Engineering and Management |
| Subjects: | |
| Online Access: | http://ehemj.com/article-1-615-en.html |
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| author | Yalda Basim Ghasemali Mohebali Sahand Jorfi Ramin Nabizadeh Mehdi Ahmadi Moghadam Ata Ghadiri Nematollah Jaafarzadeh Haghighi Fard |
| author_facet | Yalda Basim Ghasemali Mohebali Sahand Jorfi Ramin Nabizadeh Mehdi Ahmadi Moghadam Ata Ghadiri Nematollah Jaafarzadeh Haghighi Fard |
| author_sort | Yalda Basim |
| collection | DOAJ |
| description | Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to
their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was
investigated in an oil field using indigenous bacterial consortium.
Methods: The bacterial strains present in the contaminated and non-contaminated soils were
identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total
petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were
determined using a gas chromatograph equipped with a flame ionization detector (GC-FID).
Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the
families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from
those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae
(84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial
strains was less in oil-contaminated soils and varied significantly between case and control samples.
Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of
heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced
as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is
one of the innovative aspects of this research.
Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to
bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency
of 28.1% was obtained. |
| first_indexed | 2024-12-19T12:09:10Z |
| format | Article |
| id | doaj.art-dda8f8de1d3345afa66a38ef865674b5 |
| institution | Directory Open Access Journal |
| issn | 2423-3765 2423-4311 |
| language | English |
| last_indexed | 2024-12-19T12:09:10Z |
| publishDate | 2020-06-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Environmental Health Engineering and Management |
| spelling | doaj.art-dda8f8de1d3345afa66a38ef865674b52022-12-21T20:22:16ZengKerman University of Medical SciencesEnvironmental Health Engineering and Management2423-37652423-43112020-06-017212713310.34172/EHEM.2020.15Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortiumYalda Basim0Ghasemali Mohebali1Sahand Jorfi2Ramin Nabizadeh3Mehdi Ahmadi Moghadam4Ata Ghadiri5Nematollah Jaafarzadeh Haghighi Fard6Environmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMicrobiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, IranEnvironmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, IranEnvironmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranEnvironmental Technologies Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.http://ehemj.com/article-1-615-en.htmloil-contaminated soilsbiodegradationbacterial diversitytotal petroleum hydrocarbonsindigenous bacterial consortium |
| spellingShingle | Yalda Basim Ghasemali Mohebali Sahand Jorfi Ramin Nabizadeh Mehdi Ahmadi Moghadam Ata Ghadiri Nematollah Jaafarzadeh Haghighi Fard Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium Environmental Health Engineering and Management oil-contaminated soils biodegradation bacterial diversity total petroleum hydrocarbons indigenous bacterial consortium |
| title | Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium |
| title_full | Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium |
| title_fullStr | Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium |
| title_full_unstemmed | Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium |
| title_short | Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium |
| title_sort | biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium |
| topic | oil-contaminated soils biodegradation bacterial diversity total petroleum hydrocarbons indigenous bacterial consortium |
| url | http://ehemj.com/article-1-615-en.html |
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