Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents
Faecal samples from 230 diarrhoeic and healthy calves aged 0-6 weeks, from 100 farms in Austria, were examined between October 2004 and February 2005 for the presence of bacteria, especially Shiga toxin-producing Escherichia coli (STEC), viruses and parasites. Escherichia coli was detected in 17% of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2009-01-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200901-0001_characterization-of-virulence-factors-in-escherichia-coli-isolated-from-diarrheic-and-healthy-calves-in-austria.php |
| _version_ | 1797897691256061952 |
|---|---|
| author | C. Herrera-Luna D. Klein G. Lapan S. Revilla-Fernandez B. Haschek I. Sommerfeld-Stur K. Moestl W. Baumgartner |
| author_facet | C. Herrera-Luna D. Klein G. Lapan S. Revilla-Fernandez B. Haschek I. Sommerfeld-Stur K. Moestl W. Baumgartner |
| author_sort | C. Herrera-Luna |
| collection | DOAJ |
| description | Faecal samples from 230 diarrhoeic and healthy calves aged 0-6 weeks, from 100 farms in Austria, were examined between October 2004 and February 2005 for the presence of bacteria, especially Shiga toxin-producing Escherichia coli (STEC), viruses and parasites. Escherichia coli was detected in 17% of all the faecal samples and was more prevalent in healthy calves. However, E. coli F5 was identified only in one calf without diarrhoea. Overall, 35 out of the 230 (15.2%) samples analyzed carried the Shiga toxin gene: stx1, stx2 or both stx1 and stx2 in their faeces, STEC. Nevertheless, out of 39 pathogenic E. coli positive samples observed, only two carried the Shiga toxin genes: stx1, in a diarrhoeic calf and both stx1 and stx2 in a healthy calf. eaeA and Ehly genes were detected more frequently in the strains from diarrhoeic calves 57.1% and 50.0%, respectively. Clostridium perfringens was detected in twenty-one samples, the most prevalent toxin type of Clostridium perfringens was found to be type A (76.2%). Other bacteria such as Klebsiella spp. and Proteus spp. were present in 1.3% and 0.4% of all samples. Salmonella spp. was not detected. The detection rates of other enteropathogens were 25.7% bovine coronavirus, 11.7% Cryptosporidium spp., 10.4% Eimeria spp., 9.1% group A rotavirus and Giardia spp. 6.1%. We demonstrated the presence of the STEC virulence genes in healthy and diarrhoeic Austrian calves but the importance of the virulence factors of STEC (stx1, stx2, eae and Ehly) in calf diarrhoea and systemic disease is not well defined. Therefore, further studies are necessary to identify reservoirs or potential sources of virulent STEC strains in order to establish control and prevention strategies for STEC associated diseases in animals and humans. |
| first_indexed | 2024-04-10T08:02:21Z |
| format | Article |
| id | doaj.art-ddabcb5a561840d18764eac0be0b166a |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:21Z |
| publishDate | 2009-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-ddabcb5a561840d18764eac0be0b166a2023-02-23T03:49:40ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922009-01-0154111110.17221/3080-VETMEDvet-200901-0001Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agentsC. Herrera-Luna0D. Klein1G. Lapan2S. Revilla-Fernandez3B. Haschek4I. Sommerfeld-Stur5K. Moestl6W. Baumgartner7Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaDepartment for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaDepartment of Microbiology, Department of Molecular Biology, Austrian Agency for Health and Food Safety (AGES), Moedling, AustriaDepartment of Microbiology, Department of Molecular Biology, Austrian Agency for Health and Food Safety (AGES), Moedling, AustriaDepartment for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaDepartment for Biomedical Sciences, University of Veterinary Medicine, Vienna, AustriaDepartment for Pathobiology, University of Veterinary Medicine, Vienna, AustriaDepartment for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, AustriaFaecal samples from 230 diarrhoeic and healthy calves aged 0-6 weeks, from 100 farms in Austria, were examined between October 2004 and February 2005 for the presence of bacteria, especially Shiga toxin-producing Escherichia coli (STEC), viruses and parasites. Escherichia coli was detected in 17% of all the faecal samples and was more prevalent in healthy calves. However, E. coli F5 was identified only in one calf without diarrhoea. Overall, 35 out of the 230 (15.2%) samples analyzed carried the Shiga toxin gene: stx1, stx2 or both stx1 and stx2 in their faeces, STEC. Nevertheless, out of 39 pathogenic E. coli positive samples observed, only two carried the Shiga toxin genes: stx1, in a diarrhoeic calf and both stx1 and stx2 in a healthy calf. eaeA and Ehly genes were detected more frequently in the strains from diarrhoeic calves 57.1% and 50.0%, respectively. Clostridium perfringens was detected in twenty-one samples, the most prevalent toxin type of Clostridium perfringens was found to be type A (76.2%). Other bacteria such as Klebsiella spp. and Proteus spp. were present in 1.3% and 0.4% of all samples. Salmonella spp. was not detected. The detection rates of other enteropathogens were 25.7% bovine coronavirus, 11.7% Cryptosporidium spp., 10.4% Eimeria spp., 9.1% group A rotavirus and Giardia spp. 6.1%. We demonstrated the presence of the STEC virulence genes in healthy and diarrhoeic Austrian calves but the importance of the virulence factors of STEC (stx1, stx2, eae and Ehly) in calf diarrhoea and systemic disease is not well defined. Therefore, further studies are necessary to identify reservoirs or potential sources of virulent STEC strains in order to establish control and prevention strategies for STEC associated diseases in animals and humans.https://vetmed.agriculturejournals.cz/artkey/vet-200901-0001_characterization-of-virulence-factors-in-escherichia-coli-isolated-from-diarrheic-and-healthy-calves-in-austria.phpdiarrhoeacattlecalvesenteropathogensescherichia colivirulence genesshiga toxin |
| spellingShingle | C. Herrera-Luna D. Klein G. Lapan S. Revilla-Fernandez B. Haschek I. Sommerfeld-Stur K. Moestl W. Baumgartner Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents Veterinární Medicína diarrhoea cattle calves enteropathogens escherichia coli virulence genes shiga toxin |
| title | Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents |
| title_full | Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents |
| title_fullStr | Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents |
| title_full_unstemmed | Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents |
| title_short | Characterization of virulence factors in Escherichia coli isolated from diarrheic and healthy calves in Austria shedding various enteropathogenic agents |
| title_sort | characterization of virulence factors in escherichia coli isolated from diarrheic and healthy calves in austria shedding various enteropathogenic agents |
| topic | diarrhoea cattle calves enteropathogens escherichia coli virulence genes shiga toxin |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200901-0001_characterization-of-virulence-factors-in-escherichia-coli-isolated-from-diarrheic-and-healthy-calves-in-austria.php |
| work_keys_str_mv | AT cherreraluna characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT dklein characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT glapan characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT srevillafernandez characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT bhaschek characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT isommerfeldstur characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT kmoestl characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents AT wbaumgartner characterizationofvirulencefactorsinescherichiacoliisolatedfromdiarrheicandhealthycalvesinaustriasheddingvariousenteropathogenicagents |