Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i>
The applications of nanoparticles (Nps) as food additives, health enhancers, and antimicrobials in animal production are increasing. The aim of this study was to evaluate the effect of selenium (Se) nanoparticles (Se<sup>0</sup>Nps) stabilized with L-cysteine (Se<sup>0</sup>N...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/3/463 |
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| author | Francisco Yanez-Lemus Rubén Moraga Luis Mercado Carlos Jara-Gutierrez Carlos T. Smith Paulina Aguayo Kimberly Sanchez-Alonzo Apolinaria García-Cancino Ariel Valenzuela Victor L. Campos |
| author_facet | Francisco Yanez-Lemus Rubén Moraga Luis Mercado Carlos Jara-Gutierrez Carlos T. Smith Paulina Aguayo Kimberly Sanchez-Alonzo Apolinaria García-Cancino Ariel Valenzuela Victor L. Campos |
| author_sort | Francisco Yanez-Lemus |
| collection | DOAJ |
| description | The applications of nanoparticles (Nps) as food additives, health enhancers, and antimicrobials in animal production are increasing. The aim of this study was to evaluate the effect of selenium (Se) nanoparticles (Se<sup>0</sup>Nps) stabilized with L-cysteine (Se<sup>0</sup>Nps/L-Cys), as a nutritional supplement, on immunological, oxidative status, and productive parameters in <i>O. mykiss.</i> TEM and SEM-EDS showed the accumulation of spherical Se<sup>0</sup>Nps entirely composed by elemental selenium (Se<sup>0</sup>) as intracellular and extracellular deposits in <i>Pantoea agglomerans</i> UC-32 strain. The in vitro antioxidant capacity of Se<sup>0</sup>Nps/L-Cys was significant more efficient ROS scavengers than Se<sup>0</sup>Nps and Na<sub>2</sub>SeO<sub>3</sub>. We also evaluate the effect of Se<sup>0</sup>Nps/L-Cys on cell viability and oxidative stress in RTgill-W1, RTS-11, or T-PHKM <i>Oncorhynchus mykiss</i> cell lines. Se<sup>0</sup>Nps/L-Cys showed less toxic and high antioxidant activity than Se<sup>0</sup>Nps and Na<sub>2</sub>SeO<sub>3</sub>. Finally, the dietary Se<sup>0</sup>Nps/L-Cys had a significant better effect on both plasma lysozyme and respiratory burst activity (innate immune response), on tissular Gpx activity (oxidative status), and on well-being (productive parameter) of <i>O. mykiss</i> when it is compared to Se<sup>0</sup>Nps and Na<sub>2</sub>SeO<sub>3</sub>. Se<sup>0</sup>Nps/L-Cys is a promising alternative for nutritional supplement for <i>O. mykiss</i> with better performance than Na<sub>2</sub>SeO<sub>3</sub> and Se<sup>0</sup>Nps, ease to implementation, and reduced environmental impact. |
| first_indexed | 2024-03-09T13:49:32Z |
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| spelling | doaj.art-ddac9649e35442009db8cde56eb2b8b12023-11-30T20:52:34ZengMDPI AGBiology2079-77372022-03-0111346310.3390/biology11030463Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i>Francisco Yanez-Lemus0Rubén Moraga1Luis Mercado2Carlos Jara-Gutierrez3Carlos T. Smith4Paulina Aguayo5Kimberly Sanchez-Alonzo6Apolinaria García-Cancino7Ariel Valenzuela8Victor L. Campos9Environmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileMicrobiology Laboratory, Faculty of Renewable Natural Resources, Arturo Prat University, Iquique 1100000, ChileGrupo de Marcadores Inmunológicos en Organismos Acuáticos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaiso 2340000, ChileCentro de Investigaciones Biomédicas (CIB), Facultad de Medicina, Escuela de Kinesiología, Universidad de Valparaíso, Valparaiso 2340000, ChileEnvironmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileEnvironmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileLaboratory of Pisciculture and Aquatic Pathology, Department of Oceanography, Faculty of Natural and Oceanographic Sciences, Universidad de Concepcion, Concepcion 4070386, ChileEnvironmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileThe applications of nanoparticles (Nps) as food additives, health enhancers, and antimicrobials in animal production are increasing. The aim of this study was to evaluate the effect of selenium (Se) nanoparticles (Se<sup>0</sup>Nps) stabilized with L-cysteine (Se<sup>0</sup>Nps/L-Cys), as a nutritional supplement, on immunological, oxidative status, and productive parameters in <i>O. mykiss.</i> TEM and SEM-EDS showed the accumulation of spherical Se<sup>0</sup>Nps entirely composed by elemental selenium (Se<sup>0</sup>) as intracellular and extracellular deposits in <i>Pantoea agglomerans</i> UC-32 strain. The in vitro antioxidant capacity of Se<sup>0</sup>Nps/L-Cys was significant more efficient ROS scavengers than Se<sup>0</sup>Nps and Na<sub>2</sub>SeO<sub>3</sub>. We also evaluate the effect of Se<sup>0</sup>Nps/L-Cys on cell viability and oxidative stress in RTgill-W1, RTS-11, or T-PHKM <i>Oncorhynchus mykiss</i> cell lines. Se<sup>0</sup>Nps/L-Cys showed less toxic and high antioxidant activity than Se<sup>0</sup>Nps and Na<sub>2</sub>SeO<sub>3</sub>. Finally, the dietary Se<sup>0</sup>Nps/L-Cys had a significant better effect on both plasma lysozyme and respiratory burst activity (innate immune response), on tissular Gpx activity (oxidative status), and on well-being (productive parameter) of <i>O. mykiss</i> when it is compared to Se<sup>0</sup>Nps and Na<sub>2</sub>SeO<sub>3</sub>. Se<sup>0</sup>Nps/L-Cys is a promising alternative for nutritional supplement for <i>O. mykiss</i> with better performance than Na<sub>2</sub>SeO<sub>3</sub> and Se<sup>0</sup>Nps, ease to implementation, and reduced environmental impact.https://www.mdpi.com/2079-7737/11/3/463Se nanoparticles<i>Pantoea agglomerans</i>seleniterainbow troutcell viabilityantioxidant activity |
| spellingShingle | Francisco Yanez-Lemus Rubén Moraga Luis Mercado Carlos Jara-Gutierrez Carlos T. Smith Paulina Aguayo Kimberly Sanchez-Alonzo Apolinaria García-Cancino Ariel Valenzuela Victor L. Campos Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i> Biology Se nanoparticles <i>Pantoea agglomerans</i> selenite rainbow trout cell viability antioxidant activity |
| title | Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i> |
| title_full | Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i> |
| title_fullStr | Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i> |
| title_full_unstemmed | Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i> |
| title_short | Selenium Nanoparticles Biosynthesized by <i>Pantoea agglomerans</i> and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout <i>Oncorhynchus mykiss</i> |
| title_sort | selenium nanoparticles biosynthesized by i pantoea agglomerans i and their effects on cellular and physiological parameters in the rainbow trout i oncorhynchus mykiss i |
| topic | Se nanoparticles <i>Pantoea agglomerans</i> selenite rainbow trout cell viability antioxidant activity |
| url | https://www.mdpi.com/2079-7737/11/3/463 |
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