Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes
The impacts of dexamethasone (Dex) and thyroid hormone T3 on the insulin-stimulated Srebp-1c expression were studied in primary rat hepatocytes. Primary hepatocytes from Sprague-Dawley rats were isolated, cultured and treated with insulin in the presence or absence of the indicated reagents over tim...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000198 |
| _version_ | 1797701697460502528 |
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| author | Yan Zhang Xiangming Fang Fen Shuang Guoxun Chen |
| author_facet | Yan Zhang Xiangming Fang Fen Shuang Guoxun Chen |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | The impacts of dexamethasone (Dex) and thyroid hormone T3 on the insulin-stimulated Srebp-1c expression were studied in primary rat hepatocytes. Primary hepatocytes from Sprague-Dawley rats were isolated, cultured and treated with insulin in the presence or absence of the indicated reagents over time. The mRNA levels of indicated genes were determined using real-time PCR. Insulin treatment induced the Srebp-1c expression and suppressed the Pck1 expression in a time-dependent manner. Dex treatment alone reduced the Srebp-1c expression, whereas potentiated the insulin-induced its expression, which reached to a level that was higher than the insulin alone group. On the other hand, insulin treatment completely suppressed the Dex-induced Pck1 expression in the same cells. T3 treatment did not affect the expressions of Srebp-1c and Pck1 alone or in the presence of absence of insulin or Dex. Interestingly, insulin treatment induced the Rxrg mRNA expression level in the absence or presence of T0901317, a specific agonist for the liver X receptor. Dex and insulin mutually affect each other’s ability to regulate the expression levels of hepatic genes involved in glucose and fatty acid metabolism. Insulin induced Rxrg expression in primary hepatocytes, which may contribute to the induction of Srebp-1c expression in the same cells. |
| first_indexed | 2024-03-12T04:39:28Z |
| format | Article |
| id | doaj.art-ddad6d08a868486c9133554e9c053c5d |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T04:39:28Z |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-ddad6d08a868486c9133554e9c053c5d2023-09-03T09:48:12ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112515191525Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytesYan Zhang0Xiangming Fang1Fen Shuang2Guoxun Chen3Department of Gastroenterology, Affiliated Puren Hospital of Wuhan University of Science and Technology, Wuhan 430080, ChinaDepartment of Gastroenterology, Affiliated Puren Hospital of Wuhan University of Science and Technology, Wuhan 430080, ChinaDepartment of Gastroenterology, Affiliated Puren Hospital of Wuhan University of Science and Technology, Wuhan 430080, ChinaDepartment of Nutrition, University of Tennessee at Knoxville, Knoxville 37996, USA; Corresponding authors.The impacts of dexamethasone (Dex) and thyroid hormone T3 on the insulin-stimulated Srebp-1c expression were studied in primary rat hepatocytes. Primary hepatocytes from Sprague-Dawley rats were isolated, cultured and treated with insulin in the presence or absence of the indicated reagents over time. The mRNA levels of indicated genes were determined using real-time PCR. Insulin treatment induced the Srebp-1c expression and suppressed the Pck1 expression in a time-dependent manner. Dex treatment alone reduced the Srebp-1c expression, whereas potentiated the insulin-induced its expression, which reached to a level that was higher than the insulin alone group. On the other hand, insulin treatment completely suppressed the Dex-induced Pck1 expression in the same cells. T3 treatment did not affect the expressions of Srebp-1c and Pck1 alone or in the presence of absence of insulin or Dex. Interestingly, insulin treatment induced the Rxrg mRNA expression level in the absence or presence of T0901317, a specific agonist for the liver X receptor. Dex and insulin mutually affect each other’s ability to regulate the expression levels of hepatic genes involved in glucose and fatty acid metabolism. Insulin induced Rxrg expression in primary hepatocytes, which may contribute to the induction of Srebp-1c expression in the same cells.http://www.sciencedirect.com/science/article/pii/S2213453023000198InsulinPrimary hepatocytesDexamethasoneSrebp-1c expressionLipogenesis |
| spellingShingle | Yan Zhang Xiangming Fang Fen Shuang Guoxun Chen Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes Food Science and Human Wellness Insulin Primary hepatocytes Dexamethasone Srebp-1c expression Lipogenesis |
| title | Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes |
| title_full | Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes |
| title_fullStr | Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes |
| title_full_unstemmed | Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes |
| title_short | Dexamethasone potentiates the insulin-induced Srebp-1c expression in primary rat hepatocytes |
| title_sort | dexamethasone potentiates the insulin induced srebp 1c expression in primary rat hepatocytes |
| topic | Insulin Primary hepatocytes Dexamethasone Srebp-1c expression Lipogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000198 |
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