Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions
It is widely agreed that microbial biofilms play a major role in promoting infection and delaying healing of chronic wounds. In the era of microbial resistance, probiotic strains or their metabolic products are emerging as an innovative approach for the treatment of hard-to-heal (chronic) wounds due...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/2087 |
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| author | Esingül Kaya Marta Bianchi Giuseppantonio Maisetta Semih Esin Giovanna Batoni |
| author_facet | Esingül Kaya Marta Bianchi Giuseppantonio Maisetta Semih Esin Giovanna Batoni |
| author_sort | Esingül Kaya |
| collection | DOAJ |
| description | It is widely agreed that microbial biofilms play a major role in promoting infection and delaying healing of chronic wounds. In the era of microbial resistance, probiotic strains or their metabolic products are emerging as an innovative approach for the treatment of hard-to-heal (chronic) wounds due to their antimicrobial, healing, and host immune-modulatory effects. In this study, we aimed to investigate the potential of cell-free supernatants (CFS) from <i>Lacticaseibacillus rhamnosus</i> GG against mono- and dual-species biofilms of wound pathogens in a 3D in vitro infection model. Mature biofilms of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> were obtained on collagen scaffolds in the presence of a simulant wound fluid (SWF) and treated with CFS at different doses and time intervals. At 1:4 dilution in SWF, CFS caused a marked reduction in the colony forming-unit (CFU) numbers of bacteria embedded in mono-species biofilms as well as bacteria released by the biofilms in the supernatant. CFU count and electron microscopy imaging also demonstrated a marked antibiofilm effect against dual-species biofilms starting from 8 h of incubation. Furthermore, CFS exhibited acceptable levels of cytotoxicity at 24 h of incubation against HaCaT cells and, differently from ciprofloxacin, failed to induce resistance after 15 passages at sub-inhibitory concentrations. Overall, the results obtained point to <i>L. rhamnosus</i> GG postbiotics as a promising strategy for the treatment of wound biofilms. |
| first_indexed | 2024-03-07T22:30:07Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:30:07Z |
| publishDate | 2024-02-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ddb20fd75cc4458d9b2233ee72be7b122024-02-23T15:19:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254208710.3390/ijms25042087Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like ConditionsEsingül Kaya0Marta Bianchi1Giuseppantonio Maisetta2Semih Esin3Giovanna Batoni4Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyIt is widely agreed that microbial biofilms play a major role in promoting infection and delaying healing of chronic wounds. In the era of microbial resistance, probiotic strains or their metabolic products are emerging as an innovative approach for the treatment of hard-to-heal (chronic) wounds due to their antimicrobial, healing, and host immune-modulatory effects. In this study, we aimed to investigate the potential of cell-free supernatants (CFS) from <i>Lacticaseibacillus rhamnosus</i> GG against mono- and dual-species biofilms of wound pathogens in a 3D in vitro infection model. Mature biofilms of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> were obtained on collagen scaffolds in the presence of a simulant wound fluid (SWF) and treated with CFS at different doses and time intervals. At 1:4 dilution in SWF, CFS caused a marked reduction in the colony forming-unit (CFU) numbers of bacteria embedded in mono-species biofilms as well as bacteria released by the biofilms in the supernatant. CFU count and electron microscopy imaging also demonstrated a marked antibiofilm effect against dual-species biofilms starting from 8 h of incubation. Furthermore, CFS exhibited acceptable levels of cytotoxicity at 24 h of incubation against HaCaT cells and, differently from ciprofloxacin, failed to induce resistance after 15 passages at sub-inhibitory concentrations. Overall, the results obtained point to <i>L. rhamnosus</i> GG postbiotics as a promising strategy for the treatment of wound biofilms.https://www.mdpi.com/1422-0067/25/4/2087wound infections<i>Pseudomonas aeruginosa</i><i>Staphylococcus aureus</i><i>Lacticaseibacillus rhamnosus</i>resistance inductionwound model |
| spellingShingle | Esingül Kaya Marta Bianchi Giuseppantonio Maisetta Semih Esin Giovanna Batoni Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions International Journal of Molecular Sciences wound infections <i>Pseudomonas aeruginosa</i> <i>Staphylococcus aureus</i> <i>Lacticaseibacillus rhamnosus</i> resistance induction wound model |
| title | Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions |
| title_full | Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions |
| title_fullStr | Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions |
| title_full_unstemmed | Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions |
| title_short | Strong Activity and No Resistance Induction Exerted by Cell-Free Supernatants from <i>Lacticaseibacillus rhamnosus</i> against Mono-Species and Dual-Species Biofilms of Wound Pathogens in In Vivo-like Conditions |
| title_sort | strong activity and no resistance induction exerted by cell free supernatants from i lacticaseibacillus rhamnosus i against mono species and dual species biofilms of wound pathogens in in vivo like conditions |
| topic | wound infections <i>Pseudomonas aeruginosa</i> <i>Staphylococcus aureus</i> <i>Lacticaseibacillus rhamnosus</i> resistance induction wound model |
| url | https://www.mdpi.com/1422-0067/25/4/2087 |
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