Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model
Abstract Background Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining different custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involvements require more comprehensive analysis and novel nanodrugs should be...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01546-y |
| _version_ | 1798037810060460032 |
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| author | Ángela-Patricia Hernández Ania Micaelo Rafael Piñol Marina L. García-Vaquero José J. Aramayona Julio J. Criado Emilio Rodriguez José Ignacio Sánchez-Gallego Alicia Landeira-Viñuela Pablo Juanes-Velasco Paula Díez Rafael Góngora Ricardo Jara-Acevedo Alberto Orfao Javier Miana-Mena María Jesús Muñoz Sergio Villanueva Ángel Millán Manuel Fuentes |
| author_facet | Ángela-Patricia Hernández Ania Micaelo Rafael Piñol Marina L. García-Vaquero José J. Aramayona Julio J. Criado Emilio Rodriguez José Ignacio Sánchez-Gallego Alicia Landeira-Viñuela Pablo Juanes-Velasco Paula Díez Rafael Góngora Ricardo Jara-Acevedo Alberto Orfao Javier Miana-Mena María Jesús Muñoz Sergio Villanueva Ángel Millán Manuel Fuentes |
| author_sort | Ángela-Patricia Hernández |
| collection | DOAJ |
| description | Abstract Background Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining different custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involvements require more comprehensive analysis and novel nanodrugs should be characterized by both chemically and biological point of view. Within the wide variety of biocompatible nanosystems, iron oxide nanoparticles (IONPs) present mostly of the required features which make them suitable for multifunctional NPs with many biopharmaceutical applications. Results Cisplatin-IONPs and different functionalization stages have been broadly evaluated. The potential application of these nanodrugs in onco-therapies has been assessed by studying in vitro biocompatibility (interactions with environment) by proteomics characterization the determination of protein corona in different proximal fluids (human plasma, rabbit plasma and fetal bovine serum),. Moreover, protein labeling and LC–MS/MS analysis provided more than 4000 proteins de novo synthetized as consequence of the nanodrugs presence defending cell signaling in different tumor cell types (data available via ProteomeXchanges with identified PXD026615). Further in vivo studies have provided a more integrative view of the biopharmaceutical perspectives of IONPs. Conclusions Pharmacological proteomic profile different behavior between species and different affinity of protein coating layers (soft and hard corona). Also, intracellular signaling exposed differences between tumor cell lines studied. First approaches in animal model reveal the potential of theses NPs as drug delivery vehicles and confirm cisplatin compounds as strengthened antitumoral agents. |
| first_indexed | 2024-04-11T21:31:38Z |
| format | Article |
| id | doaj.art-ddb42c3347f34d46828571050cc04ce6 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T21:31:38Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-ddb42c3347f34d46828571050cc04ce62022-12-22T04:01:57ZengBMCJournal of Nanobiotechnology1477-31552022-07-0120111910.1186/s12951-022-01546-yComprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal modelÁngela-Patricia Hernández0Ania Micaelo1Rafael Piñol2Marina L. García-Vaquero3José J. Aramayona4Julio J. Criado5Emilio Rodriguez6José Ignacio Sánchez-Gallego7Alicia Landeira-Viñuela8Pablo Juanes-Velasco9Paula Díez10Rafael Góngora11Ricardo Jara-Acevedo12Alberto Orfao13Javier Miana-Mena14María Jesús Muñoz15Sergio Villanueva16Ángel Millán17Manuel Fuentes18Department of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALINMA, Institute of Nanoscience and Materials of Aragon, CSIC-University of ZaragozaDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Pharmacology and Physiology, University of ZaragozaDepartment of Inorganic Chemistry, Faculty of Chemical Sciences, Plaza de los Caídos S/NDepartment of Inorganic Chemistry, Faculty of Chemical Sciences, Plaza de los Caídos S/NDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALImmunoStep, SL, Edificio Centro de Investigación del Cáncer, University of SalamancaDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALDepartment of Pharmacology and Physiology, University of ZaragozaDepartment of Pharmacology and Physiology, University of ZaragozaDepartment of Pharmacology and Physiology, University of ZaragozaINMA, Institute of Nanoscience and Materials of Aragon, CSIC-University of ZaragozaDepartment of Medicine and General Cytometry Service-Nucleus, CIBERONC CB16/12/00400, Cancer Research Centre, (IBMCC/CSIC/USAL/IBSAL), University of Salamanca-CSIC, IBSALAbstract Background Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining different custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involvements require more comprehensive analysis and novel nanodrugs should be characterized by both chemically and biological point of view. Within the wide variety of biocompatible nanosystems, iron oxide nanoparticles (IONPs) present mostly of the required features which make them suitable for multifunctional NPs with many biopharmaceutical applications. Results Cisplatin-IONPs and different functionalization stages have been broadly evaluated. The potential application of these nanodrugs in onco-therapies has been assessed by studying in vitro biocompatibility (interactions with environment) by proteomics characterization the determination of protein corona in different proximal fluids (human plasma, rabbit plasma and fetal bovine serum),. Moreover, protein labeling and LC–MS/MS analysis provided more than 4000 proteins de novo synthetized as consequence of the nanodrugs presence defending cell signaling in different tumor cell types (data available via ProteomeXchanges with identified PXD026615). Further in vivo studies have provided a more integrative view of the biopharmaceutical perspectives of IONPs. Conclusions Pharmacological proteomic profile different behavior between species and different affinity of protein coating layers (soft and hard corona). Also, intracellular signaling exposed differences between tumor cell lines studied. First approaches in animal model reveal the potential of theses NPs as drug delivery vehicles and confirm cisplatin compounds as strengthened antitumoral agents.https://doi.org/10.1186/s12951-022-01546-yNanoparticlesCisplatinIron oxide nanoparticlesProtein coronaDrug deliveryBiocompatibility |
| spellingShingle | Ángela-Patricia Hernández Ania Micaelo Rafael Piñol Marina L. García-Vaquero José J. Aramayona Julio J. Criado Emilio Rodriguez José Ignacio Sánchez-Gallego Alicia Landeira-Viñuela Pablo Juanes-Velasco Paula Díez Rafael Góngora Ricardo Jara-Acevedo Alberto Orfao Javier Miana-Mena María Jesús Muñoz Sergio Villanueva Ángel Millán Manuel Fuentes Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model Journal of Nanobiotechnology Nanoparticles Cisplatin Iron oxide nanoparticles Protein corona Drug delivery Biocompatibility |
| title | Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model |
| title_full | Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model |
| title_fullStr | Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model |
| title_full_unstemmed | Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model |
| title_short | Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model |
| title_sort | comprehensive and systematic characterization of multi functionalized cisplatin nano conjugate from the chemistry and proteomic biocompatibility to the animal model |
| topic | Nanoparticles Cisplatin Iron oxide nanoparticles Protein corona Drug delivery Biocompatibility |
| url | https://doi.org/10.1186/s12951-022-01546-y |
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