High-resolution characterization of gene function using single-cell CRISPR tiling screen
Identifying functional domains and genetic regulatory mechanisms is essential for developing new therapies. Here the authors present sc-Tiling, single-cell high-density CRISPR tiling screening for functional domain characterization.
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24324-0 |
| _version_ | 1818405464619089920 |
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| author | Lu Yang Anthony K. N. Chan Kazuya Miyashita Christopher D. Delaney Xi Wang Hongzhi Li Sheela Pangeni Pokharel Sandra Li Mingli Li Xiaobao Xu Wei Lu Qiao Liu Nicole Mattson Kevin Yining Chen Jinhui Wang Yate-Ching Yuan David Horne Steven T. Rosen Yadira Soto-Feliciano Zhaohui Feng Takayuki Hoshii Gang Xiao Markus Müschen Jianjun Chen Scott A. Armstrong Chun-Wei Chen |
| author_facet | Lu Yang Anthony K. N. Chan Kazuya Miyashita Christopher D. Delaney Xi Wang Hongzhi Li Sheela Pangeni Pokharel Sandra Li Mingli Li Xiaobao Xu Wei Lu Qiao Liu Nicole Mattson Kevin Yining Chen Jinhui Wang Yate-Ching Yuan David Horne Steven T. Rosen Yadira Soto-Feliciano Zhaohui Feng Takayuki Hoshii Gang Xiao Markus Müschen Jianjun Chen Scott A. Armstrong Chun-Wei Chen |
| author_sort | Lu Yang |
| collection | DOAJ |
| description | Identifying functional domains and genetic regulatory mechanisms is essential for developing new therapies. Here the authors present sc-Tiling, single-cell high-density CRISPR tiling screening for functional domain characterization. |
| first_indexed | 2024-12-14T08:56:28Z |
| format | Article |
| id | doaj.art-ddb7b59f991343969e3fce23606cbf5c |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T08:56:28Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ddb7b59f991343969e3fce23606cbf5c2022-12-21T23:08:55ZengNature PortfolioNature Communications2041-17232021-07-011211910.1038/s41467-021-24324-0High-resolution characterization of gene function using single-cell CRISPR tiling screenLu Yang0Anthony K. N. Chan1Kazuya Miyashita2Christopher D. Delaney3Xi Wang4Hongzhi Li5Sheela Pangeni Pokharel6Sandra Li7Mingli Li8Xiaobao Xu9Wei Lu10Qiao Liu11Nicole Mattson12Kevin Yining Chen13Jinhui Wang14Yate-Ching Yuan15David Horne16Steven T. Rosen17Yadira Soto-Feliciano18Zhaohui Feng19Takayuki Hoshii20Gang Xiao21Markus Müschen22Jianjun Chen23Scott A. Armstrong24Chun-Wei Chen25Department of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical SchoolDepartment of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical SchoolCity of Hope Comprehensive Cancer CenterDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical SchoolCity of Hope Comprehensive Cancer CenterCity of Hope Comprehensive Cancer CenterCity of Hope Comprehensive Cancer CenterCity of Hope Comprehensive Cancer CenterRockefeller UniversityDepartment of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical SchoolDepartment of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical SchoolDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Systems Biology, Beckman Research InstituteDepartment of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical SchoolDepartment of Systems Biology, Beckman Research InstituteIdentifying functional domains and genetic regulatory mechanisms is essential for developing new therapies. Here the authors present sc-Tiling, single-cell high-density CRISPR tiling screening for functional domain characterization.https://doi.org/10.1038/s41467-021-24324-0 |
| spellingShingle | Lu Yang Anthony K. N. Chan Kazuya Miyashita Christopher D. Delaney Xi Wang Hongzhi Li Sheela Pangeni Pokharel Sandra Li Mingli Li Xiaobao Xu Wei Lu Qiao Liu Nicole Mattson Kevin Yining Chen Jinhui Wang Yate-Ching Yuan David Horne Steven T. Rosen Yadira Soto-Feliciano Zhaohui Feng Takayuki Hoshii Gang Xiao Markus Müschen Jianjun Chen Scott A. Armstrong Chun-Wei Chen High-resolution characterization of gene function using single-cell CRISPR tiling screen Nature Communications |
| title | High-resolution characterization of gene function using single-cell CRISPR tiling screen |
| title_full | High-resolution characterization of gene function using single-cell CRISPR tiling screen |
| title_fullStr | High-resolution characterization of gene function using single-cell CRISPR tiling screen |
| title_full_unstemmed | High-resolution characterization of gene function using single-cell CRISPR tiling screen |
| title_short | High-resolution characterization of gene function using single-cell CRISPR tiling screen |
| title_sort | high resolution characterization of gene function using single cell crispr tiling screen |
| url | https://doi.org/10.1038/s41467-021-24324-0 |
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