De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes
Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA ext...
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PeerJ Inc.
2016-05-01
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| Online Access: | https://peerj.com/articles/2062.pdf |
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| author | Huazong Yin Liuwang Nie Feifei Zhao Huaxing Zhou Haifeng Li Xianmei Dong Huanhuan Zhang Yuqin Wang Qiong Shi Jun Li |
| author_facet | Huazong Yin Liuwang Nie Feifei Zhao Huaxing Zhou Haifeng Li Xianmei Dong Huanhuan Zhang Yuqin Wang Qiong Shi Jun Li |
| author_sort | Huazong Yin |
| collection | DOAJ |
| description | Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle. |
| first_indexed | 2024-03-09T06:34:37Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:34:37Z |
| publishDate | 2016-05-01 |
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| record_format | Article |
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| spelling | doaj.art-ddb89d1bf214453091dbd767c890509e2023-12-03T11:01:10ZengPeerJ Inc.PeerJ2167-83592016-05-014e206210.7717/peerj.2062De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genesHuazong Yin0Liuwang Nie1Feifei Zhao2Huaxing Zhou3Haifeng Li4Xianmei Dong5Huanhuan Zhang6Yuqin Wang7Qiong Shi8Jun Li9College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaCollege of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, ChinaMauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle.https://peerj.com/articles/2062.pdfMauremys reevesiide novo assemblyRNA-seqTelomereTelomerase activityShelterin complex |
| spellingShingle | Huazong Yin Liuwang Nie Feifei Zhao Huaxing Zhou Haifeng Li Xianmei Dong Huanhuan Zhang Yuqin Wang Qiong Shi Jun Li De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes PeerJ Mauremys reevesii de novo assembly RNA-seq Telomere Telomerase activity Shelterin complex |
| title | De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes |
| title_full | De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes |
| title_fullStr | De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes |
| title_full_unstemmed | De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes |
| title_short | De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes |
| title_sort | de novo assembly and characterization of the chinese three keeled pond turtle mauremys reevesii transcriptome presence of longevity related genes |
| topic | Mauremys reevesii de novo assembly RNA-seq Telomere Telomerase activity Shelterin complex |
| url | https://peerj.com/articles/2062.pdf |
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