Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation
Aim: Broilers' optimum performance in response to their genetic potential depends on litter environment which is ideal for bacterial survival and coccidian oocyst sporulation. An in vitro evaluation was conducted for the effectiveness of superphosphate, meta-bisulfide, and charcoal litter amend...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2018-08-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.11/August-2018/8.pdf |
| _version_ | 1819141867091001344 |
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| author | Essam S. Soliman Nahla H. Sallam Eman M. Abouelhassan |
| author_facet | Essam S. Soliman Nahla H. Sallam Eman M. Abouelhassan |
| author_sort | Essam S. Soliman |
| collection | DOAJ |
| description | Aim: Broilers' optimum performance in response to their genetic potential depends on litter environment which is ideal for bacterial survival and coccidian oocyst sporulation. An in vitro evaluation was conducted for the effectiveness of superphosphate, meta-bisulfide, and charcoal litter amendments in minimizing Escherichia coli O157:H7 and Salmonella Typhimurium survival, Eimeria oocyst count, and sporulation.
Materials and Methods: Three groups of 16 litter trays were prepared and inoculated with E. coli O157:H7, S. Typhimurium, and Eimeria non-sporulated oocyst. A set of four trays in each group was designed for each one of the chemical amendments. A total of 720 litter samples were collected and examined for bacterial counts, Eimeria oocyst count, and sporulation during the experimental period (35 days).
Results: Litter moisture and pH revealed a highly significant (p<0.001) reduction in all treated litter trays compared to control. Total bacterial count (TBC), total Enterobacteriaceae count, and S. Typhimurium count showed a highly significant (p<0.001) reduction in meta-bisulfide-treated trays compared to other amendments and positive control. Meanwhile, Eimeria oocyst count and sporulation revealed a highly significant (p<0.001) reduction in superphosphate, meta-bisulfide, and charcoal-treated trays, respectively. Temperature revealed a highly significant (p<0.001) weak positive correlation with pH of all inoculated trays, a highly significant (p<0.001) weak negative correlation with moisture percentage of E. coli O157:H7 and S. Typhimurium inoculated trays, and a highly significant (p<0.001) weak negative correlation with TBC. Meanwhile, relative humidity revealed significant (p≤0.005) weak positive correlation with moisture percentage of E. coli O157:H7 inoculated trays.
Conclusion: The study concluded that regular usage with periodical reapplication of litter amendments as meta-bisulfide or superphosphate in poultry farms is one of the indispensable managemental and preventive measures for minimizing bacterial survival and inhibiting Eimeria oocyst maturation and sporulation. |
| first_indexed | 2024-12-22T12:01:16Z |
| format | Article |
| id | doaj.art-ddbc28a505f24242bb5ebfae84d4a1fe |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T12:01:16Z |
| publishDate | 2018-08-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-ddbc28a505f24242bb5ebfae84d4a1fe2022-12-21T18:26:36ZengVeterinary WorldVeterinary World0972-89882231-09162018-08-011181064107310.14202/vetworld.2018.1064-1073Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulationEssam S. Soliman0Nahla H. Sallam1Eman M. Abouelhassan2Department of Animal Hygiene, Zoonosis and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.Department of Parasitology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.Department of Parasitology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.Aim: Broilers' optimum performance in response to their genetic potential depends on litter environment which is ideal for bacterial survival and coccidian oocyst sporulation. An in vitro evaluation was conducted for the effectiveness of superphosphate, meta-bisulfide, and charcoal litter amendments in minimizing Escherichia coli O157:H7 and Salmonella Typhimurium survival, Eimeria oocyst count, and sporulation. Materials and Methods: Three groups of 16 litter trays were prepared and inoculated with E. coli O157:H7, S. Typhimurium, and Eimeria non-sporulated oocyst. A set of four trays in each group was designed for each one of the chemical amendments. A total of 720 litter samples were collected and examined for bacterial counts, Eimeria oocyst count, and sporulation during the experimental period (35 days). Results: Litter moisture and pH revealed a highly significant (p<0.001) reduction in all treated litter trays compared to control. Total bacterial count (TBC), total Enterobacteriaceae count, and S. Typhimurium count showed a highly significant (p<0.001) reduction in meta-bisulfide-treated trays compared to other amendments and positive control. Meanwhile, Eimeria oocyst count and sporulation revealed a highly significant (p<0.001) reduction in superphosphate, meta-bisulfide, and charcoal-treated trays, respectively. Temperature revealed a highly significant (p<0.001) weak positive correlation with pH of all inoculated trays, a highly significant (p<0.001) weak negative correlation with moisture percentage of E. coli O157:H7 and S. Typhimurium inoculated trays, and a highly significant (p<0.001) weak negative correlation with TBC. Meanwhile, relative humidity revealed significant (p≤0.005) weak positive correlation with moisture percentage of E. coli O157:H7 inoculated trays. Conclusion: The study concluded that regular usage with periodical reapplication of litter amendments as meta-bisulfide or superphosphate in poultry farms is one of the indispensable managemental and preventive measures for minimizing bacterial survival and inhibiting Eimeria oocyst maturation and sporulation.http://www.veterinaryworld.org/Vol.11/August-2018/8.pdfcharcoalEimeriaEscherichia colilittermeta-bisulfideSalmonellasuperphosphate |
| spellingShingle | Essam S. Soliman Nahla H. Sallam Eman M. Abouelhassan Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation Veterinary World charcoal Eimeria Escherichia coli litter meta-bisulfide Salmonella superphosphate |
| title | Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation |
| title_full | Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation |
| title_fullStr | Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation |
| title_full_unstemmed | Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation |
| title_short | Effectiveness of poultry litter amendments on bacterial survival and Eimeria oocyst sporulation |
| title_sort | effectiveness of poultry litter amendments on bacterial survival and eimeria oocyst sporulation |
| topic | charcoal Eimeria Escherichia coli litter meta-bisulfide Salmonella superphosphate |
| url | http://www.veterinaryworld.org/Vol.11/August-2018/8.pdf |
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