Viral Vectors Applied for RNAi-Based Antiviral Therapy
RNA interference (RNAi) provides the means for alternative antiviral therapy. Delivery of RNAi in the form of short interfering RNA (siRNA), short hairpin RNA (shRNA) and micro-RNA (miRNA) have demonstrated efficacy in gene silencing for therapeutic applications against viral diseases. Bioinformatic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/9/924 |
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| author | Kenneth Lundstrom |
| author_facet | Kenneth Lundstrom |
| author_sort | Kenneth Lundstrom |
| collection | DOAJ |
| description | RNA interference (RNAi) provides the means for alternative antiviral therapy. Delivery of RNAi in the form of short interfering RNA (siRNA), short hairpin RNA (shRNA) and micro-RNA (miRNA) have demonstrated efficacy in gene silencing for therapeutic applications against viral diseases. Bioinformatics has played an important role in the design of efficient RNAi sequences targeting various pathogenic viruses. However, stability and delivery of RNAi molecules have presented serious obstacles for reaching therapeutic efficacy. For this reason, RNA modifications and formulation of nanoparticles have proven useful for non-viral delivery of RNAi molecules. On the other hand, utilization of viral vectors and particularly self-replicating RNA virus vectors can be considered as an attractive alternative. In this review, examples of antiviral therapy applying RNAi-based approaches in various animal models will be described. Due to the current coronavirus pandemic, a special emphasis will be dedicated to targeting Coronavirus Disease-19 (COVID-19). |
| first_indexed | 2024-03-10T16:58:24Z |
| format | Article |
| id | doaj.art-ddbd8959fe00417395ed3c86878170d3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T16:58:24Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ddbd8959fe00417395ed3c86878170d32023-11-20T11:04:04ZengMDPI AGViruses1999-49152020-08-0112992410.3390/v12090924Viral Vectors Applied for RNAi-Based Antiviral TherapyKenneth Lundstrom0PanTherapeutics, CH1095 Lutry, SwitzerlandRNA interference (RNAi) provides the means for alternative antiviral therapy. Delivery of RNAi in the form of short interfering RNA (siRNA), short hairpin RNA (shRNA) and micro-RNA (miRNA) have demonstrated efficacy in gene silencing for therapeutic applications against viral diseases. Bioinformatics has played an important role in the design of efficient RNAi sequences targeting various pathogenic viruses. However, stability and delivery of RNAi molecules have presented serious obstacles for reaching therapeutic efficacy. For this reason, RNA modifications and formulation of nanoparticles have proven useful for non-viral delivery of RNAi molecules. On the other hand, utilization of viral vectors and particularly self-replicating RNA virus vectors can be considered as an attractive alternative. In this review, examples of antiviral therapy applying RNAi-based approaches in various animal models will be described. Due to the current coronavirus pandemic, a special emphasis will be dedicated to targeting Coronavirus Disease-19 (COVID-19).https://www.mdpi.com/1999-4915/12/9/924RNA interferenceshRNAsiRNAmiRNAgene silencingviral vectors |
| spellingShingle | Kenneth Lundstrom Viral Vectors Applied for RNAi-Based Antiviral Therapy Viruses RNA interference shRNA siRNA miRNA gene silencing viral vectors |
| title | Viral Vectors Applied for RNAi-Based Antiviral Therapy |
| title_full | Viral Vectors Applied for RNAi-Based Antiviral Therapy |
| title_fullStr | Viral Vectors Applied for RNAi-Based Antiviral Therapy |
| title_full_unstemmed | Viral Vectors Applied for RNAi-Based Antiviral Therapy |
| title_short | Viral Vectors Applied for RNAi-Based Antiviral Therapy |
| title_sort | viral vectors applied for rnai based antiviral therapy |
| topic | RNA interference shRNA siRNA miRNA gene silencing viral vectors |
| url | https://www.mdpi.com/1999-4915/12/9/924 |
| work_keys_str_mv | AT kennethlundstrom viralvectorsappliedforrnaibasedantiviraltherapy |