The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy
Abstract Background Atypical pathogen infections played an important role in community-acquired pneumonia (CAP) in children. Pathogen-specific clinical symptoms are often lacking, and it is difficult to detect atypical pathogens by culture methods. The use of multiplex polymerase chain reaction (PCR...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-01-01
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| Series: | Journal of the Egyptian Public Health Association |
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| Online Access: | http://link.springer.com/article/10.1186/s42506-018-0003-4 |
| _version_ | 1818882185356115968 |
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| author | Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy |
| author_facet | Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy |
| author_sort | Noussa R. El Basha |
| collection | DOAJ |
| description | Abstract Background Atypical pathogen infections played an important role in community-acquired pneumonia (CAP) in children. Pathogen-specific clinical symptoms are often lacking, and it is difficult to detect atypical pathogens by culture methods. The use of multiplex polymerase chain reaction (PCR) methods enables testing for many pathogens simultaneously in a single analysis. Aim To determine the role of atypical pathogens in children hospitalized with CAP. Patients and methods This cross-sectional study was conducted throughout a 2-year period from August 2015 to September 2017. It included 400 Egyptian children hospitalized with clinical diagnosis of CAP at a tertiary hospital in Cairo, Egypt. Sputum samples were collected from lower respiratory tract of all enrolled patients by mucus trap catheter for identification of Bordetella pertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophilia by using multiplex real-time PCR. Results Among the 400 CAP patients enrolled in this study, atypical pathogens were detected in 12/400 (3%) patients. Bordetella pertussis was detected in 2% of cases, and it was responsible for CAP in 8/104 (7.69%) infants in the age stratum ≤ 4 months; compared with pertussis-negative cases, pertussis-positive cases were younger and incompletely vaccinated (P values were 0.001 and 0.007, respectively). Mycoplasma pneumoniae was detected in 1% of cases, all were among the age stratum > 4 months ≤ 59 months in 4/272 (1.47%) children. Conclusion In early infancy, Bordetella pertussis causes a significant proportion of hospitalized CAP cases; all were ≤ 4 months old and incompletely vaccinated. This finding could suggest the role of maternal immunization in developing countries. |
| first_indexed | 2024-12-19T15:13:44Z |
| format | Article |
| id | doaj.art-ddbe4af279a5467ba625a7e71c280895 |
| institution | Directory Open Access Journal |
| issn | 2090-262X |
| language | English |
| last_indexed | 2024-12-19T15:13:44Z |
| publishDate | 2019-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of the Egyptian Public Health Association |
| spelling | doaj.art-ddbe4af279a5467ba625a7e71c2808952022-12-21T20:16:14ZengSpringerOpenJournal of the Egyptian Public Health Association2090-262X2019-01-019411710.1186/s42506-018-0003-4The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancyNoussa R. El Basha0Hala H. Shaaban1Hassan A. El Atroush2May M. Sherif3Amani A. El Kholy4Department of Pediatrics, Faculty of Medicine, Cairo UniversityDepartment of Pediatrics, Faculty of Medicine, Cairo UniversityDepartment of Pediatrics, Faculty of Medicine, Cairo UniversityDepartment of Clinical Pathology, Faculty of Medicine, Cairo UniversityDepartment of Clinical Pathology, Faculty of Medicine, Cairo UniversityAbstract Background Atypical pathogen infections played an important role in community-acquired pneumonia (CAP) in children. Pathogen-specific clinical symptoms are often lacking, and it is difficult to detect atypical pathogens by culture methods. The use of multiplex polymerase chain reaction (PCR) methods enables testing for many pathogens simultaneously in a single analysis. Aim To determine the role of atypical pathogens in children hospitalized with CAP. Patients and methods This cross-sectional study was conducted throughout a 2-year period from August 2015 to September 2017. It included 400 Egyptian children hospitalized with clinical diagnosis of CAP at a tertiary hospital in Cairo, Egypt. Sputum samples were collected from lower respiratory tract of all enrolled patients by mucus trap catheter for identification of Bordetella pertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophilia by using multiplex real-time PCR. Results Among the 400 CAP patients enrolled in this study, atypical pathogens were detected in 12/400 (3%) patients. Bordetella pertussis was detected in 2% of cases, and it was responsible for CAP in 8/104 (7.69%) infants in the age stratum ≤ 4 months; compared with pertussis-negative cases, pertussis-positive cases were younger and incompletely vaccinated (P values were 0.001 and 0.007, respectively). Mycoplasma pneumoniae was detected in 1% of cases, all were among the age stratum > 4 months ≤ 59 months in 4/272 (1.47%) children. Conclusion In early infancy, Bordetella pertussis causes a significant proportion of hospitalized CAP cases; all were ≤ 4 months old and incompletely vaccinated. This finding could suggest the role of maternal immunization in developing countries.http://link.springer.com/article/10.1186/s42506-018-0003-4Community-acquired pneumoniaChildrenAtypical pathogenPertussis |
| spellingShingle | Noussa R. El Basha Hala H. Shaaban Hassan A. El Atroush May M. Sherif Amani A. El Kholy The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy Journal of the Egyptian Public Health Association Community-acquired pneumonia Children Atypical pathogen Pertussis |
| title | The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
| title_full | The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
| title_fullStr | The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
| title_full_unstemmed | The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
| title_short | The use of multiplex PCR for the detection of atypical pathogens in Egyptian children with CAP: a high rate of Bordetella pertussis in early infancy |
| title_sort | use of multiplex pcr for the detection of atypical pathogens in egyptian children with cap a high rate of bordetella pertussis in early infancy |
| topic | Community-acquired pneumonia Children Atypical pathogen Pertussis |
| url | http://link.springer.com/article/10.1186/s42506-018-0003-4 |
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