New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line
Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder. Acetylcholinesterase (AChE) inhibitors constitute a pharmacotherapeutic strategy for AD treatment. Resveratrol has anti-inflammatory, neuroprotective, anticarcinogenic, and antioxidant properties. This study aimed to evaluate t...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Medical Sciences Forum |
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| Online Access: | https://www.mdpi.com/2673-9992/21/1/31 |
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| author | Martina Donozo Valeria Cavallaro Carlos Javier Baier Ana Paula Murray |
| author_facet | Martina Donozo Valeria Cavallaro Carlos Javier Baier Ana Paula Murray |
| author_sort | Martina Donozo |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder. Acetylcholinesterase (AChE) inhibitors constitute a pharmacotherapeutic strategy for AD treatment. Resveratrol has anti-inflammatory, neuroprotective, anticarcinogenic, and antioxidant properties. This study aimed to evaluate the AChE inhibitory properties and the protective role against oxidative stress damage of five resveratrol analogs (M1 to M5) in SH-SY5Y cells. The studied compounds were not cytotoxic in a wide range of concentrations. The treatment of SH-SY5Y cells at the AChE’s IC50s concentration significantly decreased the AChE enzyme activity in live cells. Through the dichlorofluorescein (DCF) assay, three compounds decreased the endogenous production of reactive oxygen species (ROS). These results demonstrate that, in addition to their action as biologically active AChE inhibitors, some resveratrol derivatives exhibit neuroprotective effects against endogenous ROS production. |
| first_indexed | 2024-03-08T20:29:27Z |
| format | Article |
| id | doaj.art-ddbe52d6d4874bc5be4afebb3c88bbb2 |
| institution | Directory Open Access Journal |
| issn | 2673-9992 |
| language | English |
| last_indexed | 2024-03-08T20:29:27Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-ddbe52d6d4874bc5be4afebb3c88bbb22023-12-22T14:28:44ZengMDPI AGMedical Sciences Forum2673-99922023-03-012113110.3390/ECB2023-14094New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell LineMartina Donozo0Valeria Cavallaro1Carlos Javier Baier2Ana Paula Murray3Instituto de Química del Sur (INQUISUR-CONICET-UNS), Departamento de Química, Universidad Nacional del Sur, Av. Alem 1253, Bahía Blanca 8000, ArgentinaInstituto de Química del Sur (INQUISUR-CONICET-UNS), Departamento de Química, Universidad Nacional del Sur, Av. Alem 1253, Bahía Blanca 8000, ArgentinaLaboratorio de Toxicología, Instituto de Ciencias Biológicas y Biomédicas del Sur (INBIOSUR-CONICET-UNS), Departamento de Biología, Bioquímica y Farmacia (DBByF), Universidad Nacional del Sur, San Juan 670, Bahía Blanca 8000, ArgentinaInstituto de Química del Sur (INQUISUR-CONICET-UNS), Departamento de Química, Universidad Nacional del Sur, Av. Alem 1253, Bahía Blanca 8000, ArgentinaAlzheimer’s disease (AD) is a multifactorial neurodegenerative disorder. Acetylcholinesterase (AChE) inhibitors constitute a pharmacotherapeutic strategy for AD treatment. Resveratrol has anti-inflammatory, neuroprotective, anticarcinogenic, and antioxidant properties. This study aimed to evaluate the AChE inhibitory properties and the protective role against oxidative stress damage of five resveratrol analogs (M1 to M5) in SH-SY5Y cells. The studied compounds were not cytotoxic in a wide range of concentrations. The treatment of SH-SY5Y cells at the AChE’s IC50s concentration significantly decreased the AChE enzyme activity in live cells. Through the dichlorofluorescein (DCF) assay, three compounds decreased the endogenous production of reactive oxygen species (ROS). These results demonstrate that, in addition to their action as biologically active AChE inhibitors, some resveratrol derivatives exhibit neuroprotective effects against endogenous ROS production.https://www.mdpi.com/2673-9992/21/1/31resveratrolAlzheimer’s diseaseacetylcholinesteraseSH-SY5Y cellsreactive oxygen species |
| spellingShingle | Martina Donozo Valeria Cavallaro Carlos Javier Baier Ana Paula Murray New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line Medical Sciences Forum resveratrol Alzheimer’s disease acetylcholinesterase SH-SY5Y cells reactive oxygen species |
| title | New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line |
| title_full | New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line |
| title_fullStr | New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line |
| title_full_unstemmed | New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line |
| title_short | New Resveratrol Derivatives Exhibit Acetylcholinesterase Inhibitory Properties and Decrease Reactive Oxygen Species Production in the SH-SY5Y Human Neuroblastoma Cell Line |
| title_sort | new resveratrol derivatives exhibit acetylcholinesterase inhibitory properties and decrease reactive oxygen species production in the sh sy5y human neuroblastoma cell line |
| topic | resveratrol Alzheimer’s disease acetylcholinesterase SH-SY5Y cells reactive oxygen species |
| url | https://www.mdpi.com/2673-9992/21/1/31 |
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