Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles
Follicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.679947/full |
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| author | Jiayuan Mo Le Sun Juanru Cheng Yujie Lu Yaochang Wei Guangsheng Qin Jing Liang Ganqiu Lan |
| author_facet | Jiayuan Mo Le Sun Juanru Cheng Yujie Lu Yaochang Wei Guangsheng Qin Jing Liang Ganqiu Lan |
| author_sort | Jiayuan Mo |
| collection | DOAJ |
| description | Follicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs) and atretic follicles (AFs) based on the follicle morphology. The expression of genes related to atresia in granulosa cells (GCs) and the concentration of hormones in the follicular fluid (FF) from HFs and AFs were detected. We then used liquid chromatography–mass spectrometry-based non-targeted metabolomic approach to analyze the metabolites in the FF from HFs and AFs. The results showed that the content of estradiol was significantly lower in AFs than in HFs, whereas that of progesterone was significantly higher in AFs than that in HFs. The expression of BCL2, VEGFA, and CYP19A1 was significantly higher in HFs than in AFs. In contrast, the expression of BAX and CASPASE3 was significantly lower in HFs. A total of 18 differential metabolites (DMs) were identified, including phospholipids, bioactive substances, and amino acids. The DMs were involved in 12 metabolic pathways, including arginine biosynthesis and primary bile acid biosynthesis. The levels of eight DMs were higher in the HF group than those in the AF group (p < 0.01), and those of 10 DMs were higher in the AF group than those in the HF group (p < 0.01). These findings indicate that the metabolic characteristics of porcine AFs are lower levels of lipids such as phospholipids and higher levels of amino acids and bile acids than those in HFs. Disorders of amino acid metabolism and cholic acid metabolism may contribute to porcine follicular atresia. |
| first_indexed | 2024-12-16T15:26:50Z |
| format | Article |
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| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-16T15:26:50Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ddbffdf262b84c2abe94392aaf33abe52022-12-21T22:26:28ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-07-01810.3389/fvets.2021.679947679947Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic FolliclesJiayuan Mo0Le Sun1Juanru Cheng2Yujie Lu3Yaochang Wei4Guangsheng Qin5Jing Liang6Ganqiu Lan7College of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaKey Laboratory of Buffalo Genetics, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Science, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaKey Laboratory of Buffalo Genetics, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Science, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaFollicular atresia is one of the main factors limiting the reproductive power of domestic animals. At present, the molecular mechanisms involved in porcine follicular atresia at the metabolic level remain unclear. In this study, we divided the follicles of Bama Xiang pigs into healthy follicles (HFs) and atretic follicles (AFs) based on the follicle morphology. The expression of genes related to atresia in granulosa cells (GCs) and the concentration of hormones in the follicular fluid (FF) from HFs and AFs were detected. We then used liquid chromatography–mass spectrometry-based non-targeted metabolomic approach to analyze the metabolites in the FF from HFs and AFs. The results showed that the content of estradiol was significantly lower in AFs than in HFs, whereas that of progesterone was significantly higher in AFs than that in HFs. The expression of BCL2, VEGFA, and CYP19A1 was significantly higher in HFs than in AFs. In contrast, the expression of BAX and CASPASE3 was significantly lower in HFs. A total of 18 differential metabolites (DMs) were identified, including phospholipids, bioactive substances, and amino acids. The DMs were involved in 12 metabolic pathways, including arginine biosynthesis and primary bile acid biosynthesis. The levels of eight DMs were higher in the HF group than those in the AF group (p < 0.01), and those of 10 DMs were higher in the AF group than those in the HF group (p < 0.01). These findings indicate that the metabolic characteristics of porcine AFs are lower levels of lipids such as phospholipids and higher levels of amino acids and bile acids than those in HFs. Disorders of amino acid metabolism and cholic acid metabolism may contribute to porcine follicular atresia.https://www.frontiersin.org/articles/10.3389/fvets.2021.679947/fullBama Xiang pigfollicular atresianon-targeted metabolomicsLC-MSfollicular fluid |
| spellingShingle | Jiayuan Mo Le Sun Juanru Cheng Yujie Lu Yaochang Wei Guangsheng Qin Jing Liang Ganqiu Lan Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles Frontiers in Veterinary Science Bama Xiang pig follicular atresia non-targeted metabolomics LC-MS follicular fluid |
| title | Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles |
| title_full | Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles |
| title_fullStr | Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles |
| title_full_unstemmed | Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles |
| title_short | Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles |
| title_sort | non targeted metabolomics reveals metabolic characteristics of porcine atretic follicles |
| topic | Bama Xiang pig follicular atresia non-targeted metabolomics LC-MS follicular fluid |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.679947/full |
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