Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research
Telomeric Repeat Binding Factors (TRFs) are architectural nuclear proteins with critical roles in telomere-length regulation, chromosome end protection and, fusion prevention, DNA damage detection, and senescence regulation. Entamoeba histolytica, the parasite responsible of human amoebiasis, harbor...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-10-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2018.00341/full |
| _version_ | 1811203359006261248 |
|---|---|
| author | Francisco Javier Rendón-Gandarilla Víctor Álvarez-Hernández Elizabeth J. Castañeda-Ortiz Helios Cárdenas-Hernández Rosa Elena Cárdenas-Guerra Jesús Valdés Abigail Betanzos Abigail Betanzos Bibiana Chávez-Munguía Anel Lagunes-Guillen Esther Orozco Lilia López-Canovas Elisa Azuara-Liceaga |
| author_facet | Francisco Javier Rendón-Gandarilla Víctor Álvarez-Hernández Elizabeth J. Castañeda-Ortiz Helios Cárdenas-Hernández Rosa Elena Cárdenas-Guerra Jesús Valdés Abigail Betanzos Abigail Betanzos Bibiana Chávez-Munguía Anel Lagunes-Guillen Esther Orozco Lilia López-Canovas Elisa Azuara-Liceaga |
| author_sort | Francisco Javier Rendón-Gandarilla |
| collection | DOAJ |
| description | Telomeric Repeat Binding Factors (TRFs) are architectural nuclear proteins with critical roles in telomere-length regulation, chromosome end protection and, fusion prevention, DNA damage detection, and senescence regulation. Entamoeba histolytica, the parasite responsible of human amoebiasis, harbors three homologs of human TRFs, based on sequence similarities to their Myb DNA binding domain. These proteins were dubbed EhTRF-like I, II and III. In this work, we revealed that EhTRF-like I and II share similarity with human TRF1, while EhTRF-like III shares similarity with human TRF2 by in silico approach. The analysis of ehtrf-like genes showed they are expressed differentially under basal culture conditions. We also studied the cellular localization of EhTRF-like I and III proteins using subcellular fractionation and western blot assays. EhTRF-like I and III proteins were enriched in the nuclear fraction, but they were also present in the cytoplasm. Indirect immunofluorescence showed that these proteins were located at the nuclear periphery co-localizing with Lamin B1 and trimethylated H4K20, which is a characteristic mark of heterochromatic regions and telomeres. We found by transmission electron microscopy that EhTRF-like III was located in regions of more condensed chromatin. Finally, EMSA assays showed that EhTRF-like III forms specific DNA-protein complexes with telomeric related sequences. Our data suggested that EhTRF-like proteins play a role in the maintenance of the chromosome ends in this parasite. |
| first_indexed | 2024-04-12T02:54:33Z |
| format | Article |
| id | doaj.art-ddc4864ddf94471da66df4d4ccebad57 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T02:54:33Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-ddc4864ddf94471da66df4d4ccebad572022-12-22T03:50:53ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882018-10-01810.3389/fcimb.2018.00341391134Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric ResearchFrancisco Javier Rendón-Gandarilla0Víctor Álvarez-Hernández1Elizabeth J. Castañeda-Ortiz2Helios Cárdenas-Hernández3Rosa Elena Cárdenas-Guerra4Jesús Valdés5Abigail Betanzos6Abigail Betanzos7Bibiana Chávez-Munguía8Anel Lagunes-Guillen9Esther Orozco10Lilia López-Canovas11Elisa Azuara-Liceaga12Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoDepartamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, MexicoConsejo Nacional de Ciencia y Tecnología, Mexico City, MexicoDepartamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, MexicoDepartamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, MexicoDepartamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, MexicoDepartamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoPosgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de Mexico, Mexico City, MexicoTelomeric Repeat Binding Factors (TRFs) are architectural nuclear proteins with critical roles in telomere-length regulation, chromosome end protection and, fusion prevention, DNA damage detection, and senescence regulation. Entamoeba histolytica, the parasite responsible of human amoebiasis, harbors three homologs of human TRFs, based on sequence similarities to their Myb DNA binding domain. These proteins were dubbed EhTRF-like I, II and III. In this work, we revealed that EhTRF-like I and II share similarity with human TRF1, while EhTRF-like III shares similarity with human TRF2 by in silico approach. The analysis of ehtrf-like genes showed they are expressed differentially under basal culture conditions. We also studied the cellular localization of EhTRF-like I and III proteins using subcellular fractionation and western blot assays. EhTRF-like I and III proteins were enriched in the nuclear fraction, but they were also present in the cytoplasm. Indirect immunofluorescence showed that these proteins were located at the nuclear periphery co-localizing with Lamin B1 and trimethylated H4K20, which is a characteristic mark of heterochromatic regions and telomeres. We found by transmission electron microscopy that EhTRF-like III was located in regions of more condensed chromatin. Finally, EMSA assays showed that EhTRF-like III forms specific DNA-protein complexes with telomeric related sequences. Our data suggested that EhTRF-like proteins play a role in the maintenance of the chromosome ends in this parasite.https://www.frontiersin.org/article/10.3389/fcimb.2018.00341/fullTRFMyb-like DNA binding domainlamin B1H4K20chromosomeDNA sequence |
| spellingShingle | Francisco Javier Rendón-Gandarilla Víctor Álvarez-Hernández Elizabeth J. Castañeda-Ortiz Helios Cárdenas-Hernández Rosa Elena Cárdenas-Guerra Jesús Valdés Abigail Betanzos Abigail Betanzos Bibiana Chávez-Munguía Anel Lagunes-Guillen Esther Orozco Lilia López-Canovas Elisa Azuara-Liceaga Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research Frontiers in Cellular and Infection Microbiology TRF Myb-like DNA binding domain lamin B1 H4K20 chromosome DNA sequence |
| title | Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research |
| title_full | Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research |
| title_fullStr | Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research |
| title_full_unstemmed | Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research |
| title_short | Telomeric Repeat-Binding Factor Homologs in Entamoeba histolytica: New Clues for Telomeric Research |
| title_sort | telomeric repeat binding factor homologs in entamoeba histolytica new clues for telomeric research |
| topic | TRF Myb-like DNA binding domain lamin B1 H4K20 chromosome DNA sequence |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2018.00341/full |
| work_keys_str_mv | AT franciscojavierrendongandarilla telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT victoralvarezhernandez telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT elizabethjcastanedaortiz telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT helioscardenashernandez telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT rosaelenacardenasguerra telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT jesusvaldes telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT abigailbetanzos telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT abigailbetanzos telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT bibianachavezmunguia telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT anellagunesguillen telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT estherorozco telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT lilialopezcanovas telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch AT elisaazuaraliceaga telomericrepeatbindingfactorhomologsinentamoebahistolyticanewcluesfortelomericresearch |