Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase
The effect of ultrasonic power, extraction temperature, compound enzyme dosage and extraction time on the yield of polysaccharide from Schisandra chinensis were investigated by single factor experiment. The ultrasonic assisted complex enzyme extraction of polysaccharides from Schisandra chinensis wa...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-04-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021090146 |
| _version_ | 1811307669113274368 |
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| author | Cheng CHEN |
| author_facet | Cheng CHEN |
| author_sort | Cheng CHEN |
| collection | DOAJ |
| description | The effect of ultrasonic power, extraction temperature, compound enzyme dosage and extraction time on the yield of polysaccharide from Schisandra chinensis were investigated by single factor experiment. The ultrasonic assisted complex enzyme extraction of polysaccharides from Schisandra chinensis was optimized by orthogonal experiment based on the results of single factor experiment. The inhibitory activity of Schisandra chinensis polysaccharide on α-glucosidase was analyzed by enzyme inhibition and fluorescence spectroscopy. The results showed that the optimum extraction parameters to achieve the highest polysaccharide yield (8.12%±0.10%) from Schisandra chinensis by ultrasonic-assisted extraction of complex enzyme were obtained under a ultrasonic power of 200 W, extraction temperature of 30 ℃, enzyme dosage of 0.20% and extraction time of 40 min. The IC50 of Schisandra chinensis polysaccharide on α-glucosidase inhibition was 27.15 μg/mL, and α-glucosidase activity was inhibited by a mixture of competitive and non competitive. Meanwhile, Schisandra chinensis polysaccharide could cause fluorescence quenching of α-glucosidase. The results provide a theoretical basis for the further development of Schisandra chinensis. |
| first_indexed | 2024-04-13T09:08:23Z |
| format | Article |
| id | doaj.art-ddc741552a77423e89887ad48d4cf1e8 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-13T09:08:23Z |
| publishDate | 2022-04-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-ddc741552a77423e89887ad48d4cf1e82022-12-22T02:52:56ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-04-0143724825410.13386/j.issn1002-0306.20210901462021090146-7Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-GlucosidaseCheng CHEN0School of Health, Jiangsu Vocational and Technical College of Economics and Trade, Jiangsu Food Safety Engineering Technology Research and Development Center, Nanjing 211168, ChinaThe effect of ultrasonic power, extraction temperature, compound enzyme dosage and extraction time on the yield of polysaccharide from Schisandra chinensis were investigated by single factor experiment. The ultrasonic assisted complex enzyme extraction of polysaccharides from Schisandra chinensis was optimized by orthogonal experiment based on the results of single factor experiment. The inhibitory activity of Schisandra chinensis polysaccharide on α-glucosidase was analyzed by enzyme inhibition and fluorescence spectroscopy. The results showed that the optimum extraction parameters to achieve the highest polysaccharide yield (8.12%±0.10%) from Schisandra chinensis by ultrasonic-assisted extraction of complex enzyme were obtained under a ultrasonic power of 200 W, extraction temperature of 30 ℃, enzyme dosage of 0.20% and extraction time of 40 min. The IC50 of Schisandra chinensis polysaccharide on α-glucosidase inhibition was 27.15 μg/mL, and α-glucosidase activity was inhibited by a mixture of competitive and non competitive. Meanwhile, Schisandra chinensis polysaccharide could cause fluorescence quenching of α-glucosidase. The results provide a theoretical basis for the further development of Schisandra chinensis.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021090146schisandra chinensispolysaccharideextractionprocessα-glucosidase |
| spellingShingle | Cheng CHEN Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase Shipin gongye ke-ji schisandra chinensis polysaccharide extraction process α-glucosidase |
| title | Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase |
| title_full | Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase |
| title_fullStr | Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase |
| title_full_unstemmed | Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase |
| title_short | Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase |
| title_sort | optimization of extraction process of schisandra chinensis polysaccharide and analysis of its inhibitory activity against α glucosidase |
| topic | schisandra chinensis polysaccharide extraction process α-glucosidase |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021090146 |
| work_keys_str_mv | AT chengchen optimizationofextractionprocessofschisandrachinensispolysaccharideandanalysisofitsinhibitoryactivityagainstaglucosidase |