Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean
Bean anthracnose caused by the hemibiotrophic fungus <i>Colletotrichum lindemuthianum</i> is one of the most important diseases of common bean (<i>Phaseolus vulgaris</i>) in the world. In the present study, the whole transcriptome of common bean infected with <i>C. lind...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/15/1995 |
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| author | Juan C. Alvarez-Diaz Richard Laugé Etienne Delannoy Stéphanie Huguet Christine Paysant-Le Roux Ariane Gratias Valérie Geffroy |
| author_facet | Juan C. Alvarez-Diaz Richard Laugé Etienne Delannoy Stéphanie Huguet Christine Paysant-Le Roux Ariane Gratias Valérie Geffroy |
| author_sort | Juan C. Alvarez-Diaz |
| collection | DOAJ |
| description | Bean anthracnose caused by the hemibiotrophic fungus <i>Colletotrichum lindemuthianum</i> is one of the most important diseases of common bean (<i>Phaseolus vulgaris</i>) in the world. In the present study, the whole transcriptome of common bean infected with <i>C. lindemuthianum</i> during compatible and incompatible interactions was characterized at 48 and 72 hpi, corresponding to the biotrophy phase of the infection cycle. Our results highlight the prominent role of pathogenesis-related (PR) genes from the PR10/Bet vI family as well as a complex interplay of different plant hormone pathways including Ethylene, Salicylic acid (SA) and Jasmonic acid pathways. Gene Ontology enrichment analysis reveals that infected common bean seedlings responded by down-regulation of photosynthesis, ubiquitination-mediated proteolysis and cell wall modifications. In infected common bean, SA biosynthesis seems to be based on the PAL pathway instead of the ICS pathway, contrarily to what is described in <i>Arabidopsis</i>. Interestingly, ~30 NLR were up-regulated in both contexts. Overall, our results suggest that the difference between the compatible and incompatible reaction is more a question of timing and strength, than a massive difference in differentially expressed genes between these two contexts. Finally, we used RT-qPCR to validate the expression patterns of several genes, and the results showed an excellent agreement with deep sequencing. |
| first_indexed | 2024-03-09T05:05:29Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T05:05:29Z |
| publishDate | 2022-07-01 |
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| series | Plants |
| spelling | doaj.art-ddc85d1942cc4280b8a7094637aec2cc2023-12-03T12:55:18ZengMDPI AGPlants2223-77472022-07-011115199510.3390/plants11151995Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common BeanJuan C. Alvarez-Diaz0Richard Laugé1Etienne Delannoy2Stéphanie Huguet3Christine Paysant-Le Roux4Ariane Gratias5Valérie Geffroy6Université Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91190 Gif sur Yvette, FranceUniversité Paris-Saclay, INRAE UR 1290 BIOGER, Av. Lucien Bretignières, BP 01, 78850 Thiverval Grignon, FranceUniversité Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91190 Gif sur Yvette, FranceUniversité Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91190 Gif sur Yvette, FranceUniversité Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91190 Gif sur Yvette, FranceUniversité Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91190 Gif sur Yvette, FranceUniversité Paris-Saclay, CNRS, INRAE, Université Evry, Institute of Plant Sciences Paris-Saclay (IPS2), 91190 Gif sur Yvette, FranceBean anthracnose caused by the hemibiotrophic fungus <i>Colletotrichum lindemuthianum</i> is one of the most important diseases of common bean (<i>Phaseolus vulgaris</i>) in the world. In the present study, the whole transcriptome of common bean infected with <i>C. lindemuthianum</i> during compatible and incompatible interactions was characterized at 48 and 72 hpi, corresponding to the biotrophy phase of the infection cycle. Our results highlight the prominent role of pathogenesis-related (PR) genes from the PR10/Bet vI family as well as a complex interplay of different plant hormone pathways including Ethylene, Salicylic acid (SA) and Jasmonic acid pathways. Gene Ontology enrichment analysis reveals that infected common bean seedlings responded by down-regulation of photosynthesis, ubiquitination-mediated proteolysis and cell wall modifications. In infected common bean, SA biosynthesis seems to be based on the PAL pathway instead of the ICS pathway, contrarily to what is described in <i>Arabidopsis</i>. Interestingly, ~30 NLR were up-regulated in both contexts. Overall, our results suggest that the difference between the compatible and incompatible reaction is more a question of timing and strength, than a massive difference in differentially expressed genes between these two contexts. Finally, we used RT-qPCR to validate the expression patterns of several genes, and the results showed an excellent agreement with deep sequencing.https://www.mdpi.com/2223-7747/11/15/1995<i>Phaseolus vulgaris</i><i>Colletotrichum lindemuthianum</i>RNA-seqtranscriptome<i>Co</i>-resistance genesresistance response |
| spellingShingle | Juan C. Alvarez-Diaz Richard Laugé Etienne Delannoy Stéphanie Huguet Christine Paysant-Le Roux Ariane Gratias Valérie Geffroy Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean Plants <i>Phaseolus vulgaris</i> <i>Colletotrichum lindemuthianum</i> RNA-seq transcriptome <i>Co</i>-resistance genes resistance response |
| title | Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean |
| title_full | Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean |
| title_fullStr | Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean |
| title_full_unstemmed | Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean |
| title_short | Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus <i>Colletotrichum lindemuthianum</i> on Common Bean |
| title_sort | genome wide transcriptomic analysis of the effects of infection with the hemibiotrophic fungus i colletotrichum lindemuthianum i on common bean |
| topic | <i>Phaseolus vulgaris</i> <i>Colletotrichum lindemuthianum</i> RNA-seq transcriptome <i>Co</i>-resistance genes resistance response |
| url | https://www.mdpi.com/2223-7747/11/15/1995 |
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