Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells
Inflammatory monocyte-derived dendritic cells (Mo-DCs) have been described in several chronic inflammatory disorders, such as rheumatoid arthritis (RA), and are suspected to play a detrimental role by fueling inflammation and skewing adaptive immune responses. However, the characterization of their...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/7/1663 |
| _version_ | 1827687831994105856 |
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| author | Frédéric Coutant Jean-Jacques Pin Pierre Miossec |
| author_facet | Frédéric Coutant Jean-Jacques Pin Pierre Miossec |
| author_sort | Frédéric Coutant |
| collection | DOAJ |
| description | Inflammatory monocyte-derived dendritic cells (Mo-DCs) have been described in several chronic inflammatory disorders, such as rheumatoid arthritis (RA), and are suspected to play a detrimental role by fueling inflammation and skewing adaptive immune responses. However, the characterization of their phenotype is still limited, as well as the comprehension of the factors that govern their differentiation. Here, we show that inflammatory Mo-DCs generated in vitro expressed a large and atypical panel of C-type lectin receptors, including isoforms of CD209 and CD206, CD303 and CD207, as well as intracellular proteins at their surfaces such as the lysosomal protein CD208. Combination of these markers allowed us to identify cells in the synovial fluid of RA patients with a close phenotype of inflammatory Mo-DCs generated in vitro. Finally, we found in coculture experiments that RA synoviocytes critically affected the phenotypic differentiation of monocytes into Mo-DCs, suggesting that the crosstalk between infiltrating monocytes and local mesenchymal cells is decisive for Mo-DCs generation. |
| first_indexed | 2024-03-10T09:43:32Z |
| format | Article |
| id | doaj.art-ddc8e0f426fd482a8117cc19e3e0a470 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T09:43:32Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-ddc8e0f426fd482a8117cc19e3e0a4702023-11-22T03:28:01ZengMDPI AGCells2073-44092021-07-01107166310.3390/cells10071663Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic CellsFrédéric Coutant0Jean-Jacques Pin1Pierre Miossec2Immunogenomics and Inflammation Research Team, University of Lyon, Edouard Herriot Hospital, 69437 Lyon, FranceEurobio Scientific/Dendritics—Edouard Herriot Hospital, 69437 Lyon, FranceImmunogenomics and Inflammation Research Team, University of Lyon, Edouard Herriot Hospital, 69437 Lyon, FranceInflammatory monocyte-derived dendritic cells (Mo-DCs) have been described in several chronic inflammatory disorders, such as rheumatoid arthritis (RA), and are suspected to play a detrimental role by fueling inflammation and skewing adaptive immune responses. However, the characterization of their phenotype is still limited, as well as the comprehension of the factors that govern their differentiation. Here, we show that inflammatory Mo-DCs generated in vitro expressed a large and atypical panel of C-type lectin receptors, including isoforms of CD209 and CD206, CD303 and CD207, as well as intracellular proteins at their surfaces such as the lysosomal protein CD208. Combination of these markers allowed us to identify cells in the synovial fluid of RA patients with a close phenotype of inflammatory Mo-DCs generated in vitro. Finally, we found in coculture experiments that RA synoviocytes critically affected the phenotypic differentiation of monocytes into Mo-DCs, suggesting that the crosstalk between infiltrating monocytes and local mesenchymal cells is decisive for Mo-DCs generation.https://www.mdpi.com/2073-4409/10/7/1663dendritic cellsrheumatoid arthritisC-type lectin receptorsDC-LAMPToll-like receptors |
| spellingShingle | Frédéric Coutant Jean-Jacques Pin Pierre Miossec Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells Cells dendritic cells rheumatoid arthritis C-type lectin receptors DC-LAMP Toll-like receptors |
| title | Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells |
| title_full | Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells |
| title_fullStr | Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells |
| title_full_unstemmed | Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells |
| title_short | Extensive Phenotype of Human Inflammatory Monocyte-Derived Dendritic Cells |
| title_sort | extensive phenotype of human inflammatory monocyte derived dendritic cells |
| topic | dendritic cells rheumatoid arthritis C-type lectin receptors DC-LAMP Toll-like receptors |
| url | https://www.mdpi.com/2073-4409/10/7/1663 |
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