Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly
To artificially construct a three-dimensional cell assembly, we investigated the availability of long-duration microdroplets that emerged near a critical point in an aqueous two-phase system (ATPS) with the hydrophilic binary polymers, polyethylene glycol (PEG), and dextran (DEX), as host containers...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2019.00044/full |
| _version_ | 1811320152818450432 |
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| author | Hiroki Sakuta Tadashi Fujimoto Yusuke Yamana Yusuke Hoda Kanta Tsumoto Kenichi Yoshikawa |
| author_facet | Hiroki Sakuta Tadashi Fujimoto Yusuke Yamana Yusuke Hoda Kanta Tsumoto Kenichi Yoshikawa |
| author_sort | Hiroki Sakuta |
| collection | DOAJ |
| description | To artificially construct a three-dimensional cell assembly, we investigated the availability of long-duration microdroplets that emerged near a critical point in an aqueous two-phase system (ATPS) with the hydrophilic binary polymers, polyethylene glycol (PEG), and dextran (DEX), as host containers. We found that erythrocytes (horse red blood cells; RBCs) and NAMRU mouse mammary gland epithelial cells (NMuMG cells) were completely and spontaneously entrapped inside DEX-rich microdroplets. RBCs and NMuMG cells were located in the interior and at the periphery of the droplets at PEG/DEX = 5%:5%. In contrast, the cells exhibited opposite localizations at PEG/DEX = 10%:5%, where, interestingly, NMuMG cells apparently assembled to achieve cell adhesion. We simply interpreted such specific localizations by considering the alternative responses of these cells to the properties of the PEG/DEX interfaces with different gradients in polymer concentrations. |
| first_indexed | 2024-04-13T12:55:15Z |
| format | Article |
| id | doaj.art-ddc95096db9c4a1b87ed14cc6512c06a |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-13T12:55:15Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-ddc95096db9c4a1b87ed14cc6512c06a2022-12-22T02:46:05ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-02-01710.3389/fchem.2019.00044418806Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular AssemblyHiroki Sakuta0Tadashi Fujimoto1Yusuke Yamana2Yusuke Hoda3Kanta Tsumoto4Kenichi Yoshikawa5Graduate School of Life and Medical Sciences, Doshisha University, Kyoto, JapanGraduate School of Life and Medical Sciences, Doshisha University, Kyoto, JapanGraduate School of Life and Medical Sciences, Doshisha University, Kyoto, JapanGraduate School of Life and Medical Sciences, Doshisha University, Kyoto, JapanDivision of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, JapanGraduate School of Life and Medical Sciences, Doshisha University, Kyoto, JapanTo artificially construct a three-dimensional cell assembly, we investigated the availability of long-duration microdroplets that emerged near a critical point in an aqueous two-phase system (ATPS) with the hydrophilic binary polymers, polyethylene glycol (PEG), and dextran (DEX), as host containers. We found that erythrocytes (horse red blood cells; RBCs) and NAMRU mouse mammary gland epithelial cells (NMuMG cells) were completely and spontaneously entrapped inside DEX-rich microdroplets. RBCs and NMuMG cells were located in the interior and at the periphery of the droplets at PEG/DEX = 5%:5%. In contrast, the cells exhibited opposite localizations at PEG/DEX = 10%:5%, where, interestingly, NMuMG cells apparently assembled to achieve cell adhesion. We simply interpreted such specific localizations by considering the alternative responses of these cells to the properties of the PEG/DEX interfaces with different gradients in polymer concentrations.https://www.frontiersin.org/article/10.3389/fchem.2019.00044/fullaqueous two-phase systemmicrodropletphase separationcell assemblyred blood cellepithelial cell |
| spellingShingle | Hiroki Sakuta Tadashi Fujimoto Yusuke Yamana Yusuke Hoda Kanta Tsumoto Kenichi Yoshikawa Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly Frontiers in Chemistry aqueous two-phase system microdroplet phase separation cell assembly red blood cell epithelial cell |
| title | Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly |
| title_full | Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly |
| title_fullStr | Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly |
| title_full_unstemmed | Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly |
| title_short | Aqueous/Aqueous Micro Phase Separation: Construction of an Artificial Model of Cellular Assembly |
| title_sort | aqueous aqueous micro phase separation construction of an artificial model of cellular assembly |
| topic | aqueous two-phase system microdroplet phase separation cell assembly red blood cell epithelial cell |
| url | https://www.frontiersin.org/article/10.3389/fchem.2019.00044/full |
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