Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats
Objective To re-examine the function of the urinary bladder in vivoas well as to determine the functional and biochemical characteristics of bladder muscarinic receptors in long-term alloxan-induced diabetes rats.Methods Two-month-old male Wistar rats were injected with alloxan and the animals showi...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
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| Series: | Einstein (São Paulo) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082015000300404&lng=en&tlng=en |
| _version_ | 1819057529213157376 |
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| author | Jeová Nina Rocha |
| author_facet | Jeová Nina Rocha |
| author_sort | Jeová Nina Rocha |
| collection | DOAJ |
| description | Objective To re-examine the function of the urinary bladder in vivoas well as to determine the functional and biochemical characteristics of bladder muscarinic receptors in long-term alloxan-induced diabetes rats.Methods Two-month-old male Wistar rats were injected with alloxan and the animals showing blood glucose levels >300mg/dL together with age-paired untreated animals were kept for 11 months. Body weight, bladder weight, blood glucose, and urinary volume over a period of 24 hours were determined in both groups of animals. A voiding cystometry in conscious control and diabetic rats was performed to determine maximal micturition pressure, micturition contraction interval and duration as well as voided and post-voiding residual volume. In addition, concentration-response curves for bethanechol in isolated bladder strips, as well as [3H]-N methyl-scopolamine binding site characteristics in bladder homogenates were determined.Results Mean bladder weight was 162.5±21.2mg versus 290±37.9mg in control and treated animals, respectively (p<0.05). Micturition contraction amplitude (34.6±4.7mmHg versus 49.6±2.5mmHg), duration (14.5±1.7 seconds versus 23.33±4.6 seconds) and interval (87.5±17.02 seconds versus 281.11±20.24 seconds) were significantly greater in alloxan diabetic rats. Voided urine volume per micturition contraction was also significantly higher in diabetic animals. However the post-voiding residual volume was not statistically different. Bethanechol potency (EC50 3µM versus 5µM) and maximal effect (31.2±5.9g/g versus 36.1±6.8g/g) in isolated bladder strips as well as number (169±4fmol/mg versus 176±3fmol/mg protein) and affinity (0.69±0.1nM versus 0.57±0.1nM) of bladder muscarinic receptors were also not statistically different.Conclusion Bladder function in vivo is altered in chronic alloxan-induced diabetes rats without changes in functional and biochemical characteristics of bladder muscarinic receptors. |
| first_indexed | 2024-12-21T13:40:45Z |
| format | Article |
| id | doaj.art-ddcb37f0a4614a3d87c2642e557651a6 |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-21T13:40:45Z |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-ddcb37f0a4614a3d87c2642e557651a62022-12-21T19:02:01ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-638513340440910.1590/S1679-45082015AO3233S1679-45082015000300404Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic ratsJeová Nina RochaObjective To re-examine the function of the urinary bladder in vivoas well as to determine the functional and biochemical characteristics of bladder muscarinic receptors in long-term alloxan-induced diabetes rats.Methods Two-month-old male Wistar rats were injected with alloxan and the animals showing blood glucose levels >300mg/dL together with age-paired untreated animals were kept for 11 months. Body weight, bladder weight, blood glucose, and urinary volume over a period of 24 hours were determined in both groups of animals. A voiding cystometry in conscious control and diabetic rats was performed to determine maximal micturition pressure, micturition contraction interval and duration as well as voided and post-voiding residual volume. In addition, concentration-response curves for bethanechol in isolated bladder strips, as well as [3H]-N methyl-scopolamine binding site characteristics in bladder homogenates were determined.Results Mean bladder weight was 162.5±21.2mg versus 290±37.9mg in control and treated animals, respectively (p<0.05). Micturition contraction amplitude (34.6±4.7mmHg versus 49.6±2.5mmHg), duration (14.5±1.7 seconds versus 23.33±4.6 seconds) and interval (87.5±17.02 seconds versus 281.11±20.24 seconds) were significantly greater in alloxan diabetic rats. Voided urine volume per micturition contraction was also significantly higher in diabetic animals. However the post-voiding residual volume was not statistically different. Bethanechol potency (EC50 3µM versus 5µM) and maximal effect (31.2±5.9g/g versus 36.1±6.8g/g) in isolated bladder strips as well as number (169±4fmol/mg versus 176±3fmol/mg protein) and affinity (0.69±0.1nM versus 0.57±0.1nM) of bladder muscarinic receptors were also not statistically different.Conclusion Bladder function in vivo is altered in chronic alloxan-induced diabetes rats without changes in functional and biochemical characteristics of bladder muscarinic receptors.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082015000300404&lng=en&tlng=enBexiga urinária/metabolismoAloxano/efeitos adversosDiabetes mellitus experimentalReceptores muscarínicosMicção/efeitos de drogasRatos Wistar |
| spellingShingle | Jeová Nina Rocha Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats Einstein (São Paulo) Bexiga urinária/metabolismo Aloxano/efeitos adversos Diabetes mellitus experimental Receptores muscarínicos Micção/efeitos de drogas Ratos Wistar |
| title | Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats |
| title_full | Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats |
| title_fullStr | Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats |
| title_full_unstemmed | Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats |
| title_short | Functional and biochemical characteristics of urinary bladder muscarinic receptors in long-term alloxan diabetic rats |
| title_sort | functional and biochemical characteristics of urinary bladder muscarinic receptors in long term alloxan diabetic rats |
| topic | Bexiga urinária/metabolismo Aloxano/efeitos adversos Diabetes mellitus experimental Receptores muscarínicos Micção/efeitos de drogas Ratos Wistar |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082015000300404&lng=en&tlng=en |
| work_keys_str_mv | AT jeovaninarocha functionalandbiochemicalcharacteristicsofurinarybladdermuscarinicreceptorsinlongtermalloxandiabeticrats |